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Absorbable hydrogel skin repair scaffold constructed on the basis of recombinant human collagen, preparation method and use method thereof

A technology for recombining collagen and human collagen, which is applied in the field of medical devices and can solve problems such as heterogeneous immune rejection and allergic reactions

Active Publication Date: 2021-04-30
常州市中辉医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although skin energy fast healing dressing (PELNAC) and double-layer artificial dermal repair material (Lando) have good therapeutic effects on deep burn wounds, traumatic skin loss, and chronic refractory wounds, but due to their main clinical The source of raw materials for the therapeutic effect is collagen extracted from animals, and there is a risk of transmission of animal xenogeneic animal viruses. Animal-derived protein materials may have shortcomings and deficiencies such as heterogeneous immune rejection and allergic reactions to the human body. ), double-layer artificial dermis repair material (Lando) requires suture and secondary surgery during treatment, and it also has its limitations for complex wounds (such as deep and shallow wounds, sinus wounds)

Method used

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  • Absorbable hydrogel skin repair scaffold constructed on the basis of recombinant human collagen, preparation method and use method thereof
  • Absorbable hydrogel skin repair scaffold constructed on the basis of recombinant human collagen, preparation method and use method thereof
  • Absorbable hydrogel skin repair scaffold constructed on the basis of recombinant human collagen, preparation method and use method thereof

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preparation example Construction

[0053] A preparation method of recombined human collagen absorbable hydrogel skin support, which comprises the following steps:

[0054] 1) dissolving the recombinant collagen raw material in water for injection to form a collagen solution;

[0055] 2) adjusting the collagen solution in step 1) to a pH of 3.0-8.0;

[0056] 3) Add a crosslinking agent to the collagen solution in step 2), and stir evenly;

[0057] 4) react to obtain the recombined human collagen absorbable hydrogel;

[0058] 5) Cutting the bulk recombined human collagen absorbable hydrogel into a size suitable for dialysis;

[0059] 6) placing the recombinant human collagen absorbable hydrogel block in phosphate buffered saline (Phosphate Buffered Saline, PBS) for dialysis to exchange the cross-linking agent;

[0060] 7) Using phosphate buffer solution containing or not containing antibacterial components, the blocky recombinant human collagen absorbable hydrogel after dialysis is crushed and granulated to ob...

Embodiment 1

[0093] Preparation of Recombinant Human Collagen Absorbable Hydrogel Skin Scaffold 1

[0094] 1) dissolving the recombinant collagen raw material in water for injection to prepare a 13% (w / w) recombinant collagen aqueous solution;

[0095] 2) adjusting the pH of the collagen solution in step 1) to 5.0;

[0096] 3) Add N-hydroxysuccinimide (NHS) to the collagen solution in step 2), add 0.075g of 10% N-hydroxysuccinimide aqueous solution according to the mass of each 1g of collagen raw material, and stir evenly;

[0097] 4) Add 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) to step 3), add 0.1g 50% 1-( 3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) aqueous solution;

[0098] 5) react for 2 hours to obtain a recombinant human collagen absorbable hydrogel;

[0099] 6) Cut the recombinant human collagen absorbable hydrogel into 1-2cm 3 small gel pieces;

[0100] 7) Dialyzing blocky recombinant human collagen absorbable hydrogel in PBS;

[01...

Embodiment 2

[0104] Preparation of Recombinant Human Collagen Absorbable Hydrogel Skin Scaffold 2

[0105] 1) dissolving the recombinant collagen raw material in water for injection to prepare a 15% (w / w) recombinant collagen aqueous solution;

[0106] 2) adjusting the pH of the collagen solution in step 1) to 5.5;

[0107] 3) Add N-hydroxysuccinimide (NHS) to the collagen solution in step 2), add 0.1 g of 10% N-hydroxysuccinimide aqueous solution according to the mass of 1 g of collagen raw material, and stir evenly;

[0108] 4) Add 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) to step 3), add 0.2g 50% 1-( 3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) aqueous solution;

[0109] 5) reacting for 4 hours to obtain blocky recombinant human collagen absorbable hydrogel;

[0110] 6) Cut the recombinant human collagen absorbable hydrogel into 1-2cm 3 small gel pieces;

[0111] 7) Dialyzing blocky recombinant human collagen absorbable hydrogel in PBS;

...

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PUM

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Abstract

The invention relates to an absorbable hydrogel skin repair scaffold constructed on the basis of recombinant human collagen, a preparation method and a use method thereof. The main structural component of the scaffold is human collagen, and the scaffold has excellent water absorption, water retention and air permeability and can provide a wet repair environment for a wound surface. When the skin is wounded, the hydrogel is smeared on the wound surface, the outer layer is covered with the medical wound dressing, and the wound surface hydrogel is physically isolated from the outside. The hydrogel not only maintains extracellular moisture, but also directly provides skin with a material which is homologous with human collagen finally generated by cell repair, and directly participates in a skin dermis extracellular matrix remodeling process, so that scars are reduced.

Description

technical field [0001] The invention belongs to the technical field of medical devices, in particular, the invention relates to a recombined human collagen absorbable hydrogel skin support and a preparation method and a use method thereof. Background technique [0002] With the development of life, production, transportation, as well as radiotherapy and chemotherapy, chronic diseases, and population aging, the skin, as the outermost physical defense barrier of the human body, is the first to suffer damage, and skin wounds often eventually heal in the form of scars. When the skin is physically damaged, the homeostasis of the skin is temporarily stopped, and it turns to wound healing and repair reactions. Full-thickness skin wounds often lead to scar formation, and the hyperplasia, sclerosis, and contracture of the scar will lead to the loss of function and deformity of the appearance of the patient. Affects the labor force and creates a burden on the family. [0003] Commerc...

Claims

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Application Information

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IPC IPC(8): A61L27/24A61L27/52A61L27/58A61L27/60
CPCA61L27/24A61L27/52A61L27/58A61L27/60C08L89/00
Inventor 朱世辉李海航吕黄红钱松
Owner 常州市中辉医疗器械有限公司
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