Absorbable hydrogel skin repair scaffold constructed on the basis of recombinant human collagen, preparation method and use method thereof
A technology for recombining collagen and human collagen, which is applied in the field of medical devices and can solve problems such as heterogeneous immune rejection and allergic reactions
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[0053] A preparation method of recombined human collagen absorbable hydrogel skin support, which comprises the following steps:
[0054] 1) dissolving the recombinant collagen raw material in water for injection to form a collagen solution;
[0055] 2) adjusting the collagen solution in step 1) to a pH of 3.0-8.0;
[0056] 3) Add a crosslinking agent to the collagen solution in step 2), and stir evenly;
[0057] 4) react to obtain the recombined human collagen absorbable hydrogel;
[0058] 5) Cutting the bulk recombined human collagen absorbable hydrogel into a size suitable for dialysis;
[0059] 6) placing the recombinant human collagen absorbable hydrogel block in phosphate buffered saline (Phosphate Buffered Saline, PBS) for dialysis to exchange the cross-linking agent;
[0060] 7) Using phosphate buffer solution containing or not containing antibacterial components, the blocky recombinant human collagen absorbable hydrogel after dialysis is crushed and granulated to ob...
Embodiment 1
[0093] Preparation of Recombinant Human Collagen Absorbable Hydrogel Skin Scaffold 1
[0094] 1) dissolving the recombinant collagen raw material in water for injection to prepare a 13% (w / w) recombinant collagen aqueous solution;
[0095] 2) adjusting the pH of the collagen solution in step 1) to 5.0;
[0096] 3) Add N-hydroxysuccinimide (NHS) to the collagen solution in step 2), add 0.075g of 10% N-hydroxysuccinimide aqueous solution according to the mass of each 1g of collagen raw material, and stir evenly;
[0097] 4) Add 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) to step 3), add 0.1g 50% 1-( 3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) aqueous solution;
[0098] 5) react for 2 hours to obtain a recombinant human collagen absorbable hydrogel;
[0099] 6) Cut the recombinant human collagen absorbable hydrogel into 1-2cm 3 small gel pieces;
[0100] 7) Dialyzing blocky recombinant human collagen absorbable hydrogel in PBS;
[01...
Embodiment 2
[0104] Preparation of Recombinant Human Collagen Absorbable Hydrogel Skin Scaffold 2
[0105] 1) dissolving the recombinant collagen raw material in water for injection to prepare a 15% (w / w) recombinant collagen aqueous solution;
[0106] 2) adjusting the pH of the collagen solution in step 1) to 5.5;
[0107] 3) Add N-hydroxysuccinimide (NHS) to the collagen solution in step 2), add 0.1 g of 10% N-hydroxysuccinimide aqueous solution according to the mass of 1 g of collagen raw material, and stir evenly;
[0108] 4) Add 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) to step 3), add 0.2g 50% 1-( 3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) aqueous solution;
[0109] 5) reacting for 4 hours to obtain blocky recombinant human collagen absorbable hydrogel;
[0110] 6) Cut the recombinant human collagen absorbable hydrogel into 1-2cm 3 small gel pieces;
[0111] 7) Dialyzing blocky recombinant human collagen absorbable hydrogel in PBS;
...
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