Primer, probe and kit for HRAS G13R mutation detection

Abstract

The invention relates to the technical field of biology, particularly to a primer, a probe and a kit for HRAS G13R mutation detection. The kit comprises allele specific primers, probes, allele non-specific primers, allele non-specific probes and PCR technology common reagents, wherein the PCR common reagents, the primers and the probes form a PCR reaction solution. The kit can effectively and accurately detect the mutation of HRAS G13R in a sample, and has high specificity and sensitivity.

Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a primer, a probe and a kit for HRAS G13R mutation detection. Background technique

[0002] Mutations in the RAS gene were first reported in cancer more than 30 years ago, and numerous studies since then have demonstrated that mutated RAS is a driver of tumor initiation and maintenance (Cox and Der, 2010). Three human RAS genes, the Kirsten murine sarcoma viral oncogene homolog (KRAS), the neuroblastoma RAS viral oncogene homolog (NRAS), and the Harvey rat sarcoma viral oncogene homolog (HRAS), encode four RAS protein. RAS proteins (KRAS4A, KRAS4B, NRAS, and HRAS) act as GDP-GTP-regulated binary switches that regulate cytoplasmic signaling networks that control various normal cellular processes. The HRAS gene is responsible for encoding a protein called H-ras, which is part of the RAS / MAPK pathway and is responsible for controlling transcriptional activity and cell cycle, and is as...

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