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Qualitative and quantitative marker for bombyx batryticatus counterfeit products and detection method thereof

A quantitative detection method and marker technology, applied in the field of authenticity identification of Chinese medicinal materials, can solve the problems of incompatibility with on-site monitoring and large-scale promotion, complex sample processing, and high requirements, and achieve good social value, good repeatability, and accuracy. good effect

Active Publication Date: 2021-04-30
JIANGSU PROVINCIAL HOSPITAL OF TCM +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Using inductively coupled plasma atomic emission spectrometry (ICPAES) and inductively coupled plasma mass spectrometry (ICP-MS) to detect borax, the sample processing is more complicated, and the requirements for the instrument are relatively high, which takes a long time and costs a lot. High, not suitable for large-scale rapid detection of counterfeit silkworm products at the grassroots level; the borax-turmeric colorimetric method in the national food inspection standard for borax (GB 5009.275-2016) uses the highly toxic chloroform as an extraction solvent, which is used in Under the action of light, it can be oxidized by oxygen in the air into hydrogen chloride and highly toxic phosgene, and it belongs to the controlled items that are prone to poisoning, so it is not suitable for on-site monitoring and large-scale promotion

Method used

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  • Qualitative and quantitative marker for bombyx batryticatus counterfeit products and detection method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0049] A qualitative and quantitative detection method for fake silkworm products, characterized in that it comprises the following steps:

[0050] Step (1) Configure the extraction and reaction solutions according to the following weights:

[0051] 2-Ethyl-1,3-hexanediol-n-butanol solution: take 10 mL of 2-ethyl-1,3-hexanediol, add n-butanol to dilute to 100 mL, and store the diluted solution in a plastic container ;

[0052] Dilute sulfuric acid solution: take equal volumes of sulfuric acid and water, slowly pour sulfuric acid into the water along the wall of the beaker, and keep stirring with a glass rod to dissipate the heat in time.

[0053] Potassium ferrocyanide solution: Weigh 10.3 g of potassium ferrocyanide, dissolve in water, and dilute to 100 mL.

[0054] Zinc acetate solution: Weigh 2.5 g of zinc acetate, add 5 mL of glacial acetic acid to dissolve it, and dilute to 100 mL with water.

[0055] Step (2) Configure the reaction solution by the following weight:

...

Embodiment 2

[0062] A qualitative and quantitative detection method for silkworm authentic products, characterized in that it comprises the following steps:

[0063] Step (1) Configure the extraction and reaction solutions according to the following weights:

[0064] 2-Ethyl-1,3-hexanediol-n-butanol solution: take 10 mL of 2-ethyl-1,3-hexanediol, add n-butanol to dilute to 100 mL, and store the diluted solution in a plastic container ;

[0065] Dilute sulfuric acid solution: take equal volumes of sulfuric acid and water, slowly pour sulfuric acid into the water along the wall of the beaker, and keep stirring with a glass rod to dissipate the heat in time.

[0066] Potassium ferrocyanide solution: Weigh 10.3 g of potassium ferrocyanide, dissolve in water, and dilute to 100 mL.

[0067] Zinc acetate solution: Weigh 2.5 g of zinc acetate, add 5 mL of glacial acetic acid to dissolve it, and dilute to 100 mL with water.

[0068] Step (2) Configure the reaction solution by the following weigh...

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Abstract

The invention discloses a qualitative and quantitative marker for bombyx batryticatus counterfeit products and a detection method thereof. The marker is borax or boric acid. According to the invention, borax in the bombyx batryticatus counterfeit product is rapidly enriched and subjected to microwave extraction through an ethyl hexanediol n-butyl alcohol solution, borax is converted into boric acid under the acidic condition, boric acid and curcumin protonated by sulfuric acid can be subjected to a chromogenic reaction, and the content of borax in the bombyx batryticatus counterfeit product can be qualitatively and quantitatively determined through a colorimetric method. According to the method, the authenticity of bombyx batryticatus can be identified through qualitative and quantitative detection of borax in counterfeit bombyx batryticatus. The method has great application prospects and social value in stiff silkworm variety authenticity identification application. The method provided by the invention selects n-butyl alcohol with low toxicity as an extraction solvent, is safe to operate, is suitable for large-scale popularization and application, has the characteristics of rapid, low-toxicity, stable, qualitative and quantitative detection of borax in bombyx batryticatus counterfeit products, and provides technical support for quality standardization and normalization of traditional Chinese medicinal materials bombyx batryticatus and clinical medication safety.

Description

technical field [0001] The invention belongs to the technical field of authenticity identification of traditional Chinese medicinal materials, and relates to a qualitative and quantitative marker of a counterfeit silkworm and a detection method thereof. Background technique [0002] The 2020 edition of the Chinese Pharmacopoeia records that Bombyx mori is a deadly dried larvae infected (or artificially inoculated) with Beauveria bassiana (Bals.) Vuillant by Bombyxmori Linnaeus 4-5 instar larvae of the silk moth family insects. About 2,000 tons of Bombyx mori are used for It is clinically used to treat diseases and is a commonly used animal traditional Chinese medicine. In recent years, the market has been flooded with a large number of dead silkworms wrapped with Beauveria bassiana spore powder instead of Bombyx mori. The existing pharmacopoeia standards are difficult to distinguish counterfeit products from the characteristics. . The silkworm of the invention can identify...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33G01N21/31
CPCG01N21/33G01N21/31Y02A50/30
Inventor 倪亮姚毅王文渊张先林刘继平旷祥张建军
Owner JIANGSU PROVINCIAL HOSPITAL OF TCM
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