Qualitative and quantitative marker for bombyx batryticatus counterfeit products and detection method thereof
A quantitative detection method and marker technology, applied in the field of authenticity identification of Chinese medicinal materials, can solve the problems of incompatibility with on-site monitoring and large-scale promotion, complex sample processing, and high requirements, and achieve good social value, good repeatability, and accuracy. good effect
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Embodiment 1
[0049] A qualitative and quantitative detection method for fake silkworm products, characterized in that it comprises the following steps:
[0050] Step (1) Configure the extraction and reaction solutions according to the following weights:
[0051] 2-Ethyl-1,3-hexanediol-n-butanol solution: take 10 mL of 2-ethyl-1,3-hexanediol, add n-butanol to dilute to 100 mL, and store the diluted solution in a plastic container ;
[0052] Dilute sulfuric acid solution: take equal volumes of sulfuric acid and water, slowly pour sulfuric acid into the water along the wall of the beaker, and keep stirring with a glass rod to dissipate the heat in time.
[0053] Potassium ferrocyanide solution: Weigh 10.3 g of potassium ferrocyanide, dissolve in water, and dilute to 100 mL.
[0054] Zinc acetate solution: Weigh 2.5 g of zinc acetate, add 5 mL of glacial acetic acid to dissolve it, and dilute to 100 mL with water.
[0055] Step (2) Configure the reaction solution by the following weight:
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Embodiment 2
[0062] A qualitative and quantitative detection method for silkworm authentic products, characterized in that it comprises the following steps:
[0063] Step (1) Configure the extraction and reaction solutions according to the following weights:
[0064] 2-Ethyl-1,3-hexanediol-n-butanol solution: take 10 mL of 2-ethyl-1,3-hexanediol, add n-butanol to dilute to 100 mL, and store the diluted solution in a plastic container ;
[0065] Dilute sulfuric acid solution: take equal volumes of sulfuric acid and water, slowly pour sulfuric acid into the water along the wall of the beaker, and keep stirring with a glass rod to dissipate the heat in time.
[0066] Potassium ferrocyanide solution: Weigh 10.3 g of potassium ferrocyanide, dissolve in water, and dilute to 100 mL.
[0067] Zinc acetate solution: Weigh 2.5 g of zinc acetate, add 5 mL of glacial acetic acid to dissolve it, and dilute to 100 mL with water.
[0068] Step (2) Configure the reaction solution by the following weigh...
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