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Method for inhibiting infectious microbe reproduction in spirulina cultivation process by using diprimary/secondary alkyl thiosulfinate

The technology of secondary alkyl thiosulfinic acid ester and secondary alkyl thiosulfinic acid is applied in the field of aquaculture and production, and can solve the problems of weak penetration, low feasibility, destruction of nutrient components and the like, To achieve the effect of increasing economic benefits and obvious economic benefits

Active Publication Date: 2021-05-04
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reported patented technology about inhibiting or killing miscellaneous bacteria in the production process of spirulina, for example: CN201621426240.9 uses high-concentration ozone water to immerse and sterilize the harvested algae mud, and the strong oxidation will inevitably damage the spirulina. It is not feasible to cause damage to various nutrient components of various nutrients; in CN201910884771.4, ultraviolet rays are used to sterilize the culture medium, the penetration of ultraviolet rays is weak, and the effect is poor, and it has almost no effect on the rapid reproduction of miscellaneous bacteria during the breeding period, so it can only be used Suitable for laboratory scale spirulina culture, not suitable for large-scale spirulina production

Method used

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  • Method for inhibiting infectious microbe reproduction in spirulina cultivation process by using diprimary/secondary alkyl thiosulfinate
  • Method for inhibiting infectious microbe reproduction in spirulina cultivation process by using diprimary/secondary alkyl thiosulfinate
  • Method for inhibiting infectious microbe reproduction in spirulina cultivation process by using diprimary/secondary alkyl thiosulfinate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Preparation of di-n-pentyl thiosulfinate:

[0028] Add di-n-pentyl disulfide in dichloromethane-methanol (dichloromethane to methanol volume ratio is 90:10) according to the molar concentration of 50mol / L, and add dimethyl selenate according to the molar concentration of 5mol / L ester; then add hydrogen peroxide according to 20mol / L, and stir the reaction at 25°C for 5 hours. After the reaction was finished, the reaction mixture was washed three times with three volumes of saturated sodium chloride solution. The product was obtained after the reaction mixture was dried under vacuum at 35 °C. The content of di-n-pentylthiosulfinate in the product was 90%.

[0029] Material Characterization: m / z 222.11; 1 HNMR (deuterated DMSO): δ=0.8ppm (triplet, 6H, -CH 3 ), 1.28~1.50ppm (multiple peaks, 12H, -CH 2 ), 2.4ppm (triplet, 2H, -CH 2 -S-), 2.5ppm (triplet, 2H, -CH 2 -S=O).

[0030] (2) Use di-n-pentyl thiosulfinate to inhibit the growth of miscellaneous bacteria in...

Embodiment 2

[0034] (1) Preparation of di-n-octyl thiosulfinate:

[0035] Add di-n-octyl disulfide (purchased from Shanghai Hansi Chemical Co., Ltd.) in dichloromethane-methanol (the volume ratio of dichloromethane and methanol is 80:20) according to the molar concentration of 35mol / L, according to the molar concentration Add dimethyl selenate at 3 mol / L; then add hydrogen peroxide at 40 mol / L, then stir and react at 50°C for 10 hours. After the reaction was finished, the reaction mixture was washed three times with three volumes of saturated sodium chloride solution. The product was obtained after the reaction mixture was dried under vacuum at 55 °C. The content of di-n-octylthiosulfinate in the product was 75%.

[0036] Material Characterization: m / z 306.21; 1 HNMR (deuterated DMSO): δ=0.88ppm (triplet, 6H, -CH 3 ), 1.21~1.50ppm (multiple peaks, 24H, -CH 2 ), 2.4ppm (triplet, 2H, -CH 2 -S-), 2.5ppm (triplet, 2H, -CH 2 -S=O).

[0037] (2) Use di-n-octyl thiosulfinate to inhibit th...

Embodiment 3

[0041] (1) Preparation of diisoamyl thiosulfinate:

[0042] In dichloromethane-methanol (the volume ratio of dichloromethane and methanol is 100:0) solution, add diisoamyl disulfide according to the molar concentration of 45mol / L, add dimethyl selenate according to 4mol / L; then After adding hydrogen peroxide at a rate of 15 mol / L, the mixture was stirred and reacted at 45° C. for 3 hours. After the reaction, the reaction mixture was washed three times with three volumes of saturated sodium chloride (purchased from Sinopharm Chemical Reagent Co., Ltd.) solution. The product was obtained after the reaction mixture was dried under vacuum at 35 °C. The content of diisoamylthiosulfinate in the product was 80%.

[0043] Material Characterization: m / z 222.11; 1 HNMR (deuterated DMSO): δ=0.93~0.99ppm (multiple peaks, 12H, -CH 3 ), 1.55ppm (multiplet, 4H, -CH 2 ), 1.77ppm (multiplet, 2H, -CH), 2.36ppm (2H, -CH 2 -S-), 2.62ppm (2H, -CH 2 -S=O).

[0044] (2) Use diisoamyl thiosul...

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Abstract

The invention discloses a method for inhibiting infectious microbe breeding in the spirulina cultivation process by utilizing diprimary / secondary alkyl thiosulfinate, and belongs to the technical field of aquatic food cultivation and production. According to the present invention, the specific substituted primary and secondary alkyl groups in the diprimary / secondary alkyl thiosulfinate are matched with the disulfide bond, such that the effects of microbial growth inhibition and infectious microbe killing are achieved, and the significant inhibition effect on the growth and the reproduction of the infectious microbe during the spirulina production is provided; meanwhile, the diprimary / secondary alkyl thiosulfinate used in the method is easy to naturally degrade, can be decomposed into harmless degradation products after staying in the water body for 1-2 days, has no shadow on the ecological system of the water body, and provides a new means for solving the problem of infectious microbe pollution in the spirulina culture production process.

Description

technical field [0001] The invention belongs to the technical field of aquatic food cultivation and production, and in particular relates to a method for inhibiting the reproduction of miscellaneous bacteria in the process of culturing spirulina by using di-primary / secondary alkyl thiosulfinate. Background technique [0002] Spirulina is an ancient photoautotrophic microalgae, named for its spiral shape under the microscope. Spirulina is an alkaline nutritious food, it contains various nutrients necessary for the human body, rich in various vitamins, trace elements, algae polysaccharides, phycocyanin, linolenic acid, insulin-like substances and other biologically active substances, these Under certain conditions, the active ingredients can lower cholesterol, detoxify the kidneys, improve the immune function of the human body, promote the synthesis of prostaglandins, suppress and prevent cancer, and accelerate wound healing and other medicinal and health functions. The Minis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N41/02A01P1/00A01P3/00C07C313/04C12N1/20C12R1/01
CPCA01N41/02C07C313/04C12N1/20
Inventor 王峰郑志永俞建峰夏晓峰陈美玲
Owner JIANGNAN UNIV
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