Method for extracting vaccinium dunalianum extract by eutectic solvent method, and preparation and application of granules

A technology of low eutectic solvent and quezui tea, which is applied in the directions of non-active ingredients medical preparations, medical preparations containing active ingredients, and drug combinations, etc., to achieve the effects of convenient operation, shortened extraction period, and simple extraction process.

Inactive Publication Date: 2021-05-04
HUAQIAO UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In addition, there are currently no reports on the method of preparing granules from the extract of sparrow mouth tea and its application in the treatment of hyperuricemia

Method used

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  • Method for extracting vaccinium dunalianum extract by eutectic solvent method, and preparation and application of granules
  • Method for extracting vaccinium dunalianum extract by eutectic solvent method, and preparation and application of granules
  • Method for extracting vaccinium dunalianum extract by eutectic solvent method, and preparation and application of granules

Examples

Experimental program
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Effect test

Embodiment 1

[0031] 1) Weigh choline chloride and anhydrous glucose according to a molar ratio of 1:4 to prepare a natural deep eutectic solvent (with a water content of 10%).

[0032] 2) Utilize the obtained natural deep eutectic solvent to extract the bird's mouth tea powder, and extract the uric acid-lowering component from the bird's mouth tea powder. The extraction method includes the following steps:

[0033] 3) Mix 20ml of deep eutectic solvent with 1g of Quezui tea powder, and perform ultrasonic extraction at 40°C. During the ultrasonic extraction process, use an ultrasonic extractor with a power of 500W and an extraction time of 10 minutes, and filter the resulting mixture.

[0034] 4) After eluting the filtrate with a deep eutectic solvent by column chromatography, separate it with 95% ethanol to obtain the sparrow mouth tea component.

Embodiment 2

[0036] 1) Weighing choline chloride and glycerol according to a molar ratio of 1:4 to prepare a natural deep eutectic solvent (with a water content of 10%);

[0037] 2) Utilize the obtained natural deep eutectic solvent to extract the bird's mouth tea powder, and extract the uric acid-lowering component from the bird's mouth tea powder. The extraction method includes the following steps:

[0038] 3) Mix 20ml of deep eutectic solvent with 1g of Quezui tea powder, and perform ultrasonic extraction at 50°C. During the ultrasonic extraction process, use an ultrasonic extractor with a power of 500W and an extraction time of 20 minutes, and filter the resulting mixture.

[0039] 4) After the eutectic solvent in the filtrate is eluted by column chromatography, the component of the sparrow's mouth tea extract is obtained by separating with 95% ethanol.

Embodiment 3

[0041] 1) Weigh choline chloride and 1,4-butanediol according to the molar ratio of 1:4 to prepare a natural deep eutectic solvent (with a water content of 10%).

[0042] 2) Utilize the obtained natural deep eutectic solvent to extract the bird's mouth tea powder, and extract the uric acid-lowering component from the bird's mouth tea powder. The extraction method includes the following steps:

[0043] 3) Mix 20ml of deep eutectic solvent with 1g of Quezui tea powder, and perform ultrasonic extraction at 60°C. During the ultrasonic extraction process, use an ultrasonic extractor with a power of 500W and an extraction time of 25 minutes, and filter the resulting mixture.

[0044] 4) After the eutectic solvent in the filtrate is eluted by column chromatography, the component of the sparrow's mouth tea extract is obtained by separating with 95% ethanol.

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Abstract

The invention provides a method for extracting a vaccinium dunalianum extract through an eutectic solvent method and preparation and application of granules of the vaccinium dunalianum extract. The method comprises the following steps: S1, crushing dried vaccinium dunalianum by using a crusher, transferring the crushed vaccinium dunalianum into a container, and storing the crushed vaccinium dunalianum in a dryer; S2, placing one of 1, 4-butanediol, anhydrous dextrose or glycerol and choline chloride in a molar ratio of 1: (4-8) in a flask, performing oil bath, and continuously performing heating and stirring to prepare an eutectic solvent; S3, mixing the vaccinium dunalianumpowder with the eutectic solvent according to the dosage that every 1g of the vaccinium dunalianum powder cooperates with 20ml of the eutectic solvent, and performing ultrasonic extraction for 30-60 minutes at the temperature of 40-80 DEG C; and S4, performing column chromatography separation on an extracting solution by using NKA-9 resin, eluting the eutectic solvent in the components, and performing separating by using 95% ethanol to obtain the vaccinium dunalianum extract. The extraction process is simple and convenient to operate, the extraction period is shortened, and the extraction efficiency is improved. The obtained vaccinium dunalianum extract can be applied to preparation of drugs for treating hyperuricemia and related diseases thereof.

Description

【Technical field】 [0001] The invention relates to a method for extracting sparrow beak tea extract by a deep eutectic solvent method and the preparation and application of granules. 【Background technique】 [0002] Hyperuricemia is a metabolic disease caused by increased uric acid synthesis and / or decreased uric acid excretion. It refers to fasting blood uric acid levels higher than 420 μmol / L twice on different days under a normal purine diet. Meta-analysis of the prevalence rate of hyperuricemia in Chinese population showed that the prevalence rate of hyperuricemia in Chinese population was 21.6% and 8.6% in males and females respectively, and the total prevalence rate was 13.3%. 130 million. [0003] As the final product of purine metabolism, uric acid is mainly synthesized in other tissues such as liver, intestine and muscle, kidney and vascular endothelium through the catalysis of xanthine oxidase, and two-thirds of it is excreted through renal uric acid transporter, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/45A61K9/16A61K47/36A61K47/26A61P19/06A61P13/12
CPCA61K9/1623A61K9/1652A61K36/45A61K2236/333A61K2236/55A61P13/12A61P19/06
Inventor 刘接卿许婧雯夏雪倩苏雨蒋宇程郝佳宝
Owner HUAQIAO UNIVERSITY
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