Method for simultaneously determining content of iridoid glycoside components in paederia scandens based on HPLC

Abstract

The invention provides a method for simultaneously determining content of iridoid glycoside components in paederia scandens based on HPLC. The method comprises the following operation steps of: a, preparing a reference substance solution: taking an iridoid glycoside reference substance, and adding an ethanol solution for dissolving the reference substance to prepare a reference substance solution; b, preparing a test solution: taking a to-be-detected sample, crushing and sieving the to-be-detected sample, adding ethanol for extraction, performing filtering, and taking a subsequent filtrate to obtain the test solution; c, separately sucking the reference solution and the test solution and injecting the reference solution and the test solution into a chromatographic instrument; and d, calculating the content of iridoid glycoside components in the to-be-detected sample by adopting an external standard method. According to the method, the contents of three effective components such as paederosidic glycosides, paederin nucleotide and asperuloside in the paederia scandens medicinal material are simultaneously determined on the basis of HPLC for the first time; and the method is stable and reliable.

Description

technical field [0001] The present invention specifically relates to a method for simultaneously determining the content of iridoid glycosides in Tachibana japonicus based on HPLC. Background technique [0002] Jiyateng is the dry aerial part of Paederia scandens (Lour.) Merr., a plant of Rubiaceae Paederia scandens (Lour.) Merr. Pain relief, dehumidification, detoxification, can be used for food accumulation, abdominal pain, eczema, sores, swelling and pain, etc. It is a commonly used folk medicine in my country. Chicken Yateng contains iridoid glycosides, flavonoids, triterpenes, volatile oils and other compounds, which have various pharmacological effects such as anti-inflammatory, analgesic, antibacterial, anti-tumor, and promotion of gastrointestinal motility. The quality standards in Sichuan and other local standards mostly focus on the inspection of properties, microscopic, thin-layer identification, moisture, ash and other routine items. Through literature search, it...

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Problems solved by technology

The quality standards in Sichuan and other local standards mostly focus on the inspection of properties, microscopic, thin-layer identification, moisture, ash and other routine items. Through literature search, it is found that the current research on Chitabaga is mostly focused on the extraction and separation of chemical components and pharmacological effects. There are relatively few reports on the content determination of the active ingredients in the medicinal material of Chiya vine

Xie Yanxiang. Content Determination and Pharmacokinetic Study of Active Components in Chickweed [D]. Guangzhou: South China University of Technology. 2018, A method for the simultaneous determination of four iridoid glycosides in Chickweed was disclosed by LC-MS / MS content method, the method is complicated, the equipment used is expensive, the detection cost is high, and it is difficult to popularize and apply

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