Method for repairing cyanide-polluted soil and underground water through in-situ leaching-ventilation enhanced microorganisms

A technology for microbial remediation and contaminated soil, applied in the field of cyanide-contaminated soil and groundwater remediation. The effect of saving the cost of medicine

Pending Publication Date: 2021-05-11
长沙工研院环保有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the chemical oxidation reaction process is too fast and it is difficult to control the actual construction, and the soil medium is easy to digest the oxidant, resulting in low oxidation efficiency
Low-temperature thermal desorption technology is more thorough in remediating cyanide-contaminated soil, but it consumes a lot of energy, and it will destroy the original soil microbial environment and affect the secondary use of soil
Chemical leaching repair technology can effectively remove free cyanide and weakly complexed cyanide in contaminated soil, but strong alkaline conditions are required for strong complexed cyanide, and it is difficult to achieve the restoration target in the case of in-situ repair, and it is easy to cause secondary pollution
Microbial remediation is suitable for remediation of low-concentration cyanide-contaminated soil. High-concentration cyanide will inhibit the ability of microorganisms to adsorb and degrade cyanide and even cause the death of microorganisms. It is difficult to guarantee the remediation effect in the actual construction process.

Method used

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  • Method for repairing cyanide-polluted soil and underground water through in-situ leaching-ventilation enhanced microorganisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Preparation of microbial inoculum: Inoculate purchased Bacillus bacteria and Trichoderma fungus on a slant, and incubate at 30°C for 18 hours. Shake flask culture medium was added with 140g / L glucose, 15g / L yeast extract, 16g / L corn steep liquor, 13g / L urea, 22g / L ammonium sulfate, 2g / L magnesium sulfate heptahydrate, 5g / L hydrogen phosphate in deionized water Dipotassium, 20g / L calcium carbonate, pH=7, shake the container until the solute dissolves, and sterilize at high temperature. Rinse the slant with sterile water and inoculate the microorganisms into the shake flask culture solution, place them in a constant temperature shaking incubator, and cultivate them for 3 days. After expanded culture, the number of Bacillus bacteria and Trichoderma fungi in the mixed bacterial solution reached 10 8 cfu / ml.

[0038] Application experiment:

[0039] The cyanide-contaminated site of an electroplating factory is mainly cyanide pollution. The average content of cyanide in th...

Embodiment 2

[0047] Preparation of microbial inoculum: Inoculate purchased bacteria of the genus Bacillus and fungi of the genus Trichoderma on a slant, and incubate at 35°C for 24 hours. Shake flask culture medium was added with 140g / L glucose, 15g / L yeast extract, 16g / L corn steep liquor, 13g / L urea, 22g / L ammonium sulfate, 2g / L magnesium sulfate heptahydrate, 5g / L hydrogen phosphate in deionized water Dipotassium, 20g / L calcium carbonate, pH=7, shake the container until the solute dissolves, and sterilize at high temperature. Rinse the slant with sterile water and inoculate the microorganisms into the shake flask culture solution, place them in a constant temperature shaking incubator, and cultivate them for 4 days. After expanded culture, the number of Bacillus bacteria and Trichoderma fungi in the mixed bacterial solution reached 10 9 cfu / ml.

[0048] Application experiment:

[0049] The cyanide-contaminated site of a coking plant is mainly cyanide pollution. The average content of...

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Abstract

The invention discloses a method for repairing cyanide-polluted soil and underground water through in-situ leaching-ventilation enhanced microorganisms. According to the method, for medium-high-concentration in-situ cyanide polluted soil and underground water, soluble cyanide in the soil is transferred to a liquid phase in an injection-extraction well alkali liquor leaching mode, and the soluble cyanide is treated and treated by ground wastewater integrated equipment and reaches the standard for recycling; pH values in the soil and the underground water are adjusted back, and ventilation is performed to maintain the oxygen content; and cyanide in the soil and the underground water is degraded by adopting cyanide degrading bacteria, so that the content of cyanide in the soil reaches the first-class land use screening value standard in Soil Pollution Risk Screening Standard for Soil Environmental Quality Construction (Trial) (GB36600-2018), and the content of cyanide in the underground water reaches the III-class standard of Quality Standard for Underground Water (GB / T14848-2017).

Description

technical field [0001] The invention relates to a method for remediating cyanide-contaminated soil and groundwater, in particular to a method for in-situ leaching-ventilation enhanced microbial remediation of cyanide-contaminated soil and groundwater, and belongs to the field of remediation of cyanide-contaminated soil and groundwater. Background technique [0002] Cyanide specifically refers to compounds with cyano groups (CN), which are extremely toxic substances that can enter the human body through the respiratory tract or digestive tract and combine with ferric iron in cytochrome oxidase in the body, so that cells cannot use oxygen and lose The function of transmitting oxygen, eventually leading to the death of the body due to hypoxia. However, because cyanide has good complexation, surface activity, and activation properties, in recent years, with the development of industries such as gold mining, smelting, electroplating, organic chemicals, and mineral processing, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/08B09C1/10B09C1/00
CPCB09C1/08B09C1/10B09C1/002B09C1/005B09C2101/00
Inventor 韩凤刘卫国邵乐史学峰李昌武王季斐
Owner 长沙工研院环保有限公司
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