Method for repairing cyanide-polluted soil and underground water through in-situ leaching-ventilation enhanced microorganisms
A technology for microbial remediation and contaminated soil, applied in the field of cyanide-contaminated soil and groundwater remediation. The effect of saving the cost of medicine
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Embodiment 1
[0037] Preparation of microbial inoculum: Inoculate purchased Bacillus bacteria and Trichoderma fungus on a slant, and incubate at 30°C for 18 hours. Shake flask culture medium was added with 140g / L glucose, 15g / L yeast extract, 16g / L corn steep liquor, 13g / L urea, 22g / L ammonium sulfate, 2g / L magnesium sulfate heptahydrate, 5g / L hydrogen phosphate in deionized water Dipotassium, 20g / L calcium carbonate, pH=7, shake the container until the solute dissolves, and sterilize at high temperature. Rinse the slant with sterile water and inoculate the microorganisms into the shake flask culture solution, place them in a constant temperature shaking incubator, and cultivate them for 3 days. After expanded culture, the number of Bacillus bacteria and Trichoderma fungi in the mixed bacterial solution reached 10 8 cfu / ml.
[0038] Application experiment:
[0039] The cyanide-contaminated site of an electroplating factory is mainly cyanide pollution. The average content of cyanide in th...
Embodiment 2
[0047] Preparation of microbial inoculum: Inoculate purchased bacteria of the genus Bacillus and fungi of the genus Trichoderma on a slant, and incubate at 35°C for 24 hours. Shake flask culture medium was added with 140g / L glucose, 15g / L yeast extract, 16g / L corn steep liquor, 13g / L urea, 22g / L ammonium sulfate, 2g / L magnesium sulfate heptahydrate, 5g / L hydrogen phosphate in deionized water Dipotassium, 20g / L calcium carbonate, pH=7, shake the container until the solute dissolves, and sterilize at high temperature. Rinse the slant with sterile water and inoculate the microorganisms into the shake flask culture solution, place them in a constant temperature shaking incubator, and cultivate them for 4 days. After expanded culture, the number of Bacillus bacteria and Trichoderma fungi in the mixed bacterial solution reached 10 9 cfu / ml.
[0048] Application experiment:
[0049] The cyanide-contaminated site of a coking plant is mainly cyanide pollution. The average content of...
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