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Small peptide for promoting osteoblast proliferation

A technology for osteoblasts and osteoblast activity, applied in the field of small peptides that promote osteoblast proliferation, can solve problems such as side effects of calcitonin

Active Publication Date: 2021-05-18
NANJING UNIV OF FINANCE & ECONOMICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the drugs for the treatment of osteoporosis mainly act on the bone resorption of osteoclasts, while there are few drugs targeting the proliferation of osteoblasts to promote bone formation. And currently on the market anti-osteoporosis drugs such as bisphosphonates, selective estrogen receptor modulators and calcitonin generally have serious side effects

Method used

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  • Small peptide for promoting osteoblast proliferation
  • Small peptide for promoting osteoblast proliferation
  • Small peptide for promoting osteoblast proliferation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1 prepares the method for the active peptide that promotes osteoblast proliferation

[0026] A method for preparing an active peptide that promotes osteoblast proliferation, comprising the steps of:

[0027] (1) Enzymatic hydrolysis of papain

[0028] Soybean protein isolate (Shanghai Yuanye Biotechnology Co., Ltd., product number S30914) was weighed and dissolved in ultrapure water to prepare a protein solution with a concentration of 4.5% by mass, and NaOH solution was added to adjust the pH to 7.0. According to the ratio of adding 3000U papain per gram of protein, papain was added to the protein solution, and then placed on a rotary mixer, and the enzymolysis reaction was carried out at 55°C for 5 hours. After testing, the hydrolysis degree of soybean protein isolate was 11.08± 0.31%. After the reaction, raise the temperature to 85°C and keep it warm for 20 minutes to inactivate papain, cool to room temperature, centrifuge at 3000×g for 15 minutes, take ...

Embodiment 7

[0044] The effect of embodiment 7 small peptide A on osteoblast ALP (alkaline phosphatase) activity

[0045] Alkaline phosphatase kit was used to detect the effect of small peptide A on ALP activity of osteoblasts. The specific method is as follows: after digesting osteoblast MC3T3-E1, use α-MEM culture solution to prepare the concentration of 1×10 5 cells / mL of cell suspension, inoculated in a 6-well plate, 2 mL per well. After the MC3T3-E1 cells grew to confluence, the culture medium was removed, and they were cultured in mineralization-inducing medium (ODM purchased from Sigma, USA, with a final concentration of 50 μg / mL ascorbic acid and 4 mM β-glycerophosphate) 7 and 10 days to induce differentiation, then replace the serum-free medium, and add small peptide A with a final concentration of 20 μM for 48 hours, and set 3 parallels. At the same time, a control group was set up, without small peptide A intervention, and the others remained unchanged. The ALP activity was d...

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Abstract

The invention provides a small peptide for promoting osteoblast proliferation, and belongs to the technical field of food processing. The small peptide has proliferation promoting activity on osteoblasts, and has an amino acid sequence shown as SEQ ID NO: 1. The small peptide is derived from soybean protein, has good safety, has a significant effect of promoting osteoblast proliferation, and can significantly promote the differentiation of the osteoblasts, so that the small peptide is expected to be used for preparing drugs or functional food for promoting osteoblast proliferation and resisting osteoporosis. The small peptide is convenient to synthesize, can be industrially produced, and has good application prospects in the fields of food, medicine, cosmetics and the like.

Description

technical field [0001] The invention relates to a small peptide for promoting proliferation of osteoblasts, which belongs to the technical field of food processing. Background technique [0002] Osteoporosis is a metabolic disease characterized by decreased bone mass, bone tissue degeneration, and microstructural destruction leading to decreased bone strength and toughness. Osteoporosis and its complications such as fractures have increasingly become a social problem that endangers public health. [0003] At present, the drugs for the treatment of osteoporosis mainly act on the bone resorption of osteoclasts, while there are few drugs targeting the proliferation of osteoblasts to promote bone formation, and the anti-osteoporosis drugs currently on the market, such as bisphosphonates, Serious side effects are common with selective estrogen receptor modulators and calcitonin. Therefore, the development of highly effective, safe, and less side effect natural product ingredien...

Claims

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Application Information

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IPC IPC(8): C07K7/06A61K38/08A61P19/10A61P19/08A23L33/18
CPCC07K7/06A61P19/10A61P19/08A23L33/18A61K38/00A23V2002/00A23V2200/306A23V2250/55
Inventor 沈新春汪芳宋海昭李宇
Owner NANJING UNIV OF FINANCE & ECONOMICS