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Preparation method of a delivery system for crossing the blood-brain barrier and specifically targeting glioma therapeutic drugs

A technology for the treatment of glioma and drugs, applied in the field of medicine, can solve the problems of low survival rate, median survival time of only 12-15 months, poor targeting, etc., and achieve the effect of improving the treatment effect

Active Publication Date: 2022-07-29
SHENZHEN INST OF ADVANCED TECH
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  • Claims
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AI Technical Summary

Problems solved by technology

At present, the standard clinical treatments mainly include surgical resection and chemotherapy. The 2-year survival rate after treatment is less than 30%, the 5-year survival rate is less than 10%, and the median survival time is only 12-15 months.
The main reason is that the BBB prevents most of the therapeutic drugs from entering the glioma tissue and poor drug targeting

Method used

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  • Preparation method of a delivery system for crossing the blood-brain barrier and specifically targeting glioma therapeutic drugs
  • Preparation method of a delivery system for crossing the blood-brain barrier and specifically targeting glioma therapeutic drugs
  • Preparation method of a delivery system for crossing the blood-brain barrier and specifically targeting glioma therapeutic drugs

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Embodiment 1

[0032]1) Preparation of NK cell membranes: Add the collected NK cells to a cell lysis buffer containing protease inhibitors, and sonicate in an ice bath for 20-30 min. The maximum centrifugal force of the broken cell centrifuge is 3500 g, and the temperature is 4 ℃, collect the supernatant after centrifugation for 10 minutes, and then continue to centrifuge the supernatant for 25 minutes under the conditions of a maximum centrifugal force of 20,000 g and a temperature of 4 ℃ to collect the supernatant, and then centrifuge the supernatant at a maximum centrifugal force of 100,000 g and a temperature of 4 ℃. Centrifuge at 4°C for 50 min, and the resulting precipitate is NK cell membranes (NKCMs);

[0033] 2) Weigh 10mg 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol) 2000] (ammonium salt) and 2mg AIEgens photothermal agent molecules into 5mL pure Mixed in water, then ultrasonicated for 5 min to obtain liposome nanoparticles (D-NPs) loaded with photot...

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Abstract

The invention discloses a preparation method of a delivery system for crossing the blood-brain barrier and specifically targeting glioma therapeutic drugs, comprising the following steps: step 1) preparation of NK cell membrane: adding NK cells to cells containing protease inhibitors In the lysis buffer, through ultrasonic centrifugation, NK cell membrane is obtained; Step 2) 1,2-distearoyl-sn-glyceryl-3-phosphoethanolamine-N-[amino (polyethylene glycol) 2000] ( Ammonium salt) and drugs are added into pure water, ultrasonicated to obtain liposome nanoparticles; step 3) NK cell membrane and liposome nanoparticles are co-extruded through a polycarbonate membrane with a pore size of 220 nm to form NK cell biomimetic nanocarriers. The NK cell biomimetic nanocarrier prepared by the invention is a delivery system for crossing the blood-brain barrier and specifically targeting glioma therapeutic drugs.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a preparation method of a delivery system for crossing the blood-brain barrier and specifically targeting glioma therapeutic drugs. Background technique [0002] The blood-brain barrier (BBB) ​​refers to the barrier between the plasma and brain cells formed by the wall of brain capillaries and glial cells, and the barrier between the plasma and cerebrospinal fluid formed by the choroid plexus. These substances enter the brain tissue from the blood. A variety of solutes in the blood enter the brain tissue from the brain capillaries, which can be difficult or easy; some pass quickly, some slowly, and some cannot pass through at all. [0003] The blood-brain barrier is a general term for the three barriers of blood-brain, blood-cerebrospinal fluid and cerebrospinal fluid-brain. Brain capillaries lack the pores that normal capillaries have, or these pores are few and small. Endothelial cel...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/69A61K45/00A61K41/00A61K49/00A61P35/00B82Y5/00B82Y30/00B82Y40/00A61K9/127
CPCA61K47/6901A61K45/00B82Y5/00B82Y30/00B82Y40/00A61P35/00A61K41/0052A61K49/0013A61K49/0097A61K9/127A61K41/00A61K47/69A61K49/00A61K47/42
Inventor 蔡林涛邓冠军孙枝红龚萍
Owner SHENZHEN INST OF ADVANCED TECH
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