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Electrochemical sensor for detecting ciprofloxacin and detection method thereof

A ciprofloxacin and sensor technology, which is applied in the direction of electrochemical variables of materials and resistance to vector-borne diseases, etc., can solve the problems of electrode modification materials and complex synthesis process, the influence of interfering substances cannot be excluded, and the accuracy of detection results is reduced, etc. , to achieve the effect of low cost, increased current value, and improved accuracy

Active Publication Date: 2021-05-28
SHANDONG AGRICULTURAL UNIVERSITY
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  • Claims
  • Application Information

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Problems solved by technology

If the structural analogs of ciprofloxacin appear in the tested sample matrix, the method reported above cannot exclude the influence of interfering substances, which will reduce the accuracy of the test results
In addition, the electrode modification materials and synthesis process used in the above reported method are relatively complicated, and high temperature heating is also required.

Method used

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  • Electrochemical sensor for detecting ciprofloxacin and detection method thereof
  • Electrochemical sensor for detecting ciprofloxacin and detection method thereof
  • Electrochemical sensor for detecting ciprofloxacin and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] 1. Modification of electrochemical sensor electrodes:

[0071] 10 μL of COF@CB@MPDA dispersion at a concentration of 1.5 mg / mL was drop-coated on the surface of the bare glassy carbon electrode and dried at room temperature to obtain a modified electrode (COF@CB@MPDA / GCE).

[0072] 2. The saturated calomel electrode and the platinum electrode were used as the reference electrode and the auxiliary electrode respectively, and the modified electrode COF@CB@MPDA / GCE obtained in this experiment was used as the working electrode to form a three-electrode system. Within the range of 0.7-1.3V, immerse the working electrode in ciprofloxacin standard solutions with concentrations of 0.5, 1, 5, 10, 20, 50 and 100μmol / L for incubation, and record the corresponding current values ​​I P .

[0073]3. Take the concentration of ciprofloxacin standard solution as the abscissa, and the current value as the ordinate, and draw the working curve. Calculate the content of ciprofloxacin in t...

Embodiment 2

[0077] Embodiment 2: the specificity of detection method of the present invention:

[0078] In order to explore the specific recognition ability of COF@CB@MPDA to ciprofloxacin, this example selects Enoxacin (Enoxacin), Moxifloxacin (Moxifloxacin) and Enrofloxacin ( Enrofloxacin) three structural analogues were tested for selectivity.

[0079] Figure 5 is the peak current value of COF@CB@MPDA / GCE and COF@CB@NPDA / GCE in 50mM ciprofloxacin and three structural analogues at equal concentrations; the synthesis method of COF@CB@NPDA is the same as that of COF@CB@ Compared with the synthesis of MPDA, except that ciprofloxacin was not added, the rest of the synthesis steps were the same. It was calculated that the imprinting factors of COF@CB@MPDA / GCE to ciprofloxacin, enoxacin, moxifloxacin and enrofloxacin were 1.98, 1.19, 0.76 and 1.10, respectively. It can be seen that COF@CB@MPDA / GCE only has good specific recognition ability for ciprofloxacin.

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Abstract

The invention discloses an electrochemical sensor for detecting ciprofloxacin and a detection method thereof, and belongs to the technical field of food safety detection. According to the electrochemical sensor, an electrode modified by a COF@CB@MPDA composite material is used as a working electrode; the COF@CB@MPDA composite material is prepared by the following method: dispersing COF and CB into double distilled water to obtain a mixed solution; adding ciprofloxacin and dopamine into the mixed solution according to a molar ratio of 1: (9 to 12), uniformly dispersing, adding a Tris-HCl (pH 8.5) solution, stirring and reacting at room temperature for 10 to 14 hours, and washing and drying the obtained product to obtain the COF@CB@MPDA composite material. The lowest detection limit of the electrochemical sensor to ciprofloxacin is 9.46 [mu] g / kg, and detection requirements can be met; the pretreatment time and the analysis time are respectively shortened by 48 minutes and 10 minutes compared with those of a traditional detection method, and the method is suitable for rapid detection of ciprofloxacin.

Description

technical field [0001] The invention relates to the technical field of food safety detection, in particular to an electrochemical sensor for detecting ciprofloxacin and a detection method thereof. Background technique [0002] Ciprofloxacin (CIP) is a synthetic third-generation fluoroquinolone antibacterial drug, and its structural formula is as follows: [0003] [0004] Ciprofloxacin has broad-spectrum antibacterial activity, and has a good effect on both Gram-positive bacteria and Gram-negative bacteria. Therefore, ciprofloxacin is often used to treat digestive and respiratory infections, as well as urinary tract infections. Ciprofloxacin can inhibit bacterial DNA helicase, prevent bacterial replication, and rapidly reduce bacterial reproduction. [0005] However, ciprofloxacin sometimes causes allergic reactions, phlebitis, gastrointestinal tract reactions and central nervous system reactions in humans. The Ministry of Agriculture of the People's Republic of China ...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30
CPCG01N27/26G01N27/30Y02A50/30
Inventor 徐志祥孙玉奉陈永峰张鸿雁孔非凡
Owner SHANDONG AGRICULTURAL UNIVERSITY
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