Nucleic acid aptamer-quantum dot biosensor for detecting and killing salmonella and preparation method and application thereof

A Salmonella nucleic acid and nucleic acid aptamer technology, which is applied in measurement devices, biological testing, biological material analysis, etc., can solve the problems of high requirements for instruments and equipment, cumbersome detection methods, incomplete sterilization, etc., and achieves high sensitivity and significant sterilization effect. , use a wide range of effects

Active Publication Date: 2021-06-01
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned biosensor and sterilization method of the present invention can solve the problems of low sensitivity, cumbersome detection met

Method used

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  • Nucleic acid aptamer-quantum dot biosensor for detecting and killing salmonella and preparation method and application thereof
  • Nucleic acid aptamer-quantum dot biosensor for detecting and killing salmonella and preparation method and application thereof
  • Nucleic acid aptamer-quantum dot biosensor for detecting and killing salmonella and preparation method and application thereof

Examples

Experimental program
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Example Embodiment

[0044]Example 1

[0045]Nucleic acid fittings for Salmonella detection and killing - quantum dot biosensor, is in depositing a PDA coating3O4The surface of the nanoparticle is attached to the carbon mendomype labeled Salmoneli nucleic acid fitting Aptamer C (SEQ IDNO.1); and the complementary chain Strand A (SEQ ID NO. 2) of the modified fluorescein FAM is paired with the above Aptamer C;

[0046]The Salmoneli nucleic acid fitting Aptamer C 5 'modified carboxyl group, 3' modified with -SH, as follows:

[0047]5'-cooh-agcagcacag-gcgatccaagcttcttca-sh-3 '

[0048]The complementary chain STRAND A of the modified fluorescein FAM is as follows:

[0049]3'-CGCTAGGTTCGAAGAAGT-FAM-5 '

[0050]The carbon quantum dot is carbon quantum dots having a particle size of 2-3 nm in a hydrothermal method, and a good fluorescent performance in a hydrothermal process.

[0051]The preparation method of the nucleic acid adapter-quantum dot biosensor for Salmonella detection and murder, the steps are as follows:

[0052](1) In t...

Example Embodiment

[0058]Example 2

[0059]A Salmonella detection and murder method, steps below:

[0060]Example 1 The nucleic acid body ligand-quantum dot biosensor is dispersed in phosphate buffered saline, and the solution is obtained into a solution having a concentration of 0.32 μm, and the dispersion is added to the food surface for detecting Salmonella, Salmonella will be due to high affinity The role is specific to the Aptamer C strand, and the A chain will compete by bacteria, and the carbon quantum dots on the Aptamer C chain will show blue green fluorescence due to the loss of fluorescein FAM on the A chain. By detecting blue-green fluorescence of carbon quantum dots for determining the content of Salmonella;

[0061]Using 1.5W / cm in the area where the fluorescence is displayed2 808 nm near infrared light continuously; after the finish, the food is placed in a magnetic field or separated from Feature under the action of the magnet.3O4Nanoparticles, and modify the surface of the food with FAM STRA...

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Abstract

The invention discloses a nucleic acid aptamer-quantum dot biosensor for detecting and killing salmonella and a preparation method and application thereof, and belongs to the technical field of detection and inactivation of food pathogenic bacteria. The biosensor disclosed by the invention is prepared by connecting a salmonella aptamer Aptamer C marked by quantum dots to the surfaces of Fe3O4 nanoparticles deposited with a polydopamine (PDA) coating; pairing a complementary chain Strand A modified with fluorescein with the Aptamer C, and pairing a complementary chain Strand A modified with fluorescein with the Aptamer C; using the Fe3O4 nano particles connected with the Aptamer C and the Srand A for detecting the salmonella. The detection sensitivity of the biosensor is 10 CFU/100 g, the detection range is 10-10< 4 > CFU/100 g, and the biosensor has good sensitivity and specificity; the sterilization degree reaches 95% or above, and the effect is remarkable.

Description

technical field [0001] The invention belongs to the technical field of detection and deactivation of food pathogenic bacteria, and in particular relates to a nucleic acid aptamer-quantum dot biosensor for detection and deactivation of Salmonella, its preparation method and application. Background technique [0002] Food pathogens are pathogenic bacteria that can cause food poisoning or use food as a medium of transmission. Pathogenic bacteria directly or indirectly contaminate food and water sources, and human oral infection can lead to the prevalence of intestinal infectious diseases, food poisoning, and livestock and poultry infectious diseases. Therefore, foodborne pathogens are an important source of food safety problems. [0003] The impact of food-borne pathogens on human health: (1) Acute poisoning: In general, food-borne pathogens often cause acute poisoning, and the milder ones often appear as symptoms of acute gastroenteritis, such as vomiting, nausea, Abdominal ...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N33/569G01N33/58G01N33/543A23L5/20A23B4/01
CPCG01N21/6428G01N33/56916G01N33/588G01N33/54346A23L5/21A23B4/01G01N2021/6432G01N2021/6439G01N2333/255A23V2002/00A23V2300/24A23V2200/10Y02A50/30
Inventor 吴薇孙霏霏杨庆利唐娟
Owner QINGDAO AGRI UNIV
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