Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for producing antibiotic substitute by fermenting Luzhou-flavor distillers' grains with compound probiotics

A technology of compound probiotics and Luzhou-flavored liquor, applied in the direction of bacteria, application, lactobacillus, etc. used in food preparation, can solve the problems of easy deterioration, high moisture content, difficult transportation, etc., and achieve the number of viable bacteria and related enzymes Excellent activity, significant effect, protein and amino acid enhancement effect

Pending Publication Date: 2021-06-04
HENAN UNIV OF SCI & TECH
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Luzhou-flavor distiller's grains have a high water content, are easy to deteriorate, and are not easy to transport, which severely limits its application range.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The method for producing an antibiotic substitute by fermenting Luzhou-flavor distiller's grains with compound probiotics, the specific steps include the following steps:

[0030] 1. Raw material pretreatment:

[0031] The Luzhou-flavor white distiller's grains adjusted to pH 7 are dried and pretreated in a drum drying equipment, and the drying temperature is controlled at 400°C to obtain distiller's grains with a moisture content of 50%. After drying and pretreatment, Luzhou-flavor white distiller's grains The miscellaneous bacteria in distiller's grains are killed, and its moisture content is suitable for adding corn flour, urea and potassium dihydrogen phosphate mixed solution in the subsequent process to control the appropriate water content, which is helpful for subsequent fermentation.

[0032] 2. Mix one ton of distiller's grains with a moisture content of 50% obtained in step 1 with 5% cornmeal by weight, and spray 50L of treatment solution in the distiller's gr...

example 2

[0039] The steps in this example are basically the same as those in Example 1, except that in step 1, the pH is adjusted to 6.5, and the drying temperature is 350°C to obtain distiller's grains with a moisture content of 50%; in step 2, in the treatment liquid Urea is 0.2g / mL, potassium dihydrogen phosphate is 0.05g / mL, every ton adds 50L treatment liquid, supplements appropriate water and makes material water content be 58%; In step 4, Bacillus coagulans (CGMCC9551), Saccharomyces cerevisiae, plant The inoculum amounts of Lactobacillus (HZLp-005) and Bacillus subtilis (CGMCC17305) were 2% respectively, stirred evenly, and the temperature of the fermentation process was 31°C.

[0040] Detect the live bacteria content of the finished probiotics produced in this example, the true protein content, fiber content and amino acid content of the dry material, and the activity of glucoamylase, cellulase and protease in the finished product, and list the test data in Table 1.

Embodiment 3

[0042] The steps in this example are basically the same as those in Example 1, except that in step 1, the pH is adjusted to 7.5, and the drying temperature is 450°C to obtain distiller’s grains with a moisture content of 50%; in step 2, the Urea is 0.3g / mL, potassium dihydrogen phosphate is 0.15g / mL, every ton adds 50L treatment liquid, supplements appropriate water and makes material water content be 62%; In step 4, bacillus coagulans (CGMCC9551), brewer's yeast, plant The inoculum amounts of Lactobacillus (HZLp-005) and Bacillus subtilis (CGMCC17305) were 3% respectively, stirred evenly, and the temperature of the fermentation process was 35°C.

[0043] Detect the live bacteria content of the finished probiotics made in this embodiment, the true protein content, fiber content and amino acid content of the dry material, and list the test data in Table 1.

[0044] The detection of the true protein content, fiber content and amino acid content of the dry material of the initial...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for producing an antibiotic substitute by fermenting Luzhou-flavor distillers' grains with compound probiotics, and belongs to the field of solid fermention. The method comprises the following steps that S1, raw material pretreatment: adjusting the pH of Luzhou-flavor distillers' grains, and conducting drying to obtain distillers' grains with a certain water content; S2, improvement before raw material fermentation: mixing vinasse with corn flour, and spraying the vinasse with a mixed treatment solution of urea and monopotassium phosphate; S3, performing activation and propagation on lactobacillus plantarum, bacillus coagulans, saccharomyces cerevisiae and bacillus subtilis in respective liquid culture media; and S4, inoculated fermentation: inoculating bacillus coagulans and a saccharomyces cerevisiae solution into a fermentation material, carrying out uniform stirring, carrying out temperature-controlled fermentation for two days, inoculating lactobacillus plantarum and bacillus subtilis into the material, performing uniform stirring, and carrying out temperature-controlled fermentation to finally obtain the antibiotic substitute. The technology is simple, the antibiotic substitute with high viable organism content and rich nutrition is obtained through the method, and efficient and high-quality utilization of vinasse is facilitated.

Description

technical field [0001] The invention belongs to the technical field of solid fermentation, and in particular relates to a method for producing antibiotic substitutes by using compound probiotics to ferment Luzhou-flavor distiller's grains. Background technique [0002] Due to the pollution of antibiotics to the environment and the threat of drug-resistant bacteria, countries have introduced relevant laws and regulations to prohibit the addition of antibiotics to feed. The complete prohibition of adding antibiotics will bring continuous challenges to the breeding industry. Therefore, people continue to study the technology of replacing antibiotics in the feed formulation and breeding process and find effective alternative products. Among the many potential antibiotic alternative products, the most popular The best alternatives to antibiotics and the most effective feed additives are organic acids, probiotics and enzymes. [0003] Although the research and development of heal...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/38A23K10/30A23K20/105A23K20/26A23K10/12
CPCA23K10/38A23K10/30A23K20/105A23K20/26A23K10/12A23V2400/169Y02P60/87
Inventor 古绍彬崔玉明张献敏吴影何昭惠赵丽娜李璇马金亮
Owner HENAN UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com