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Extraction method of centella asiatica extract with high biological safety

A centella asiatica extract, biosafety technology, applied in the direction of pharmaceutical formulations, steroids, plant raw materials, etc., to achieve the effect of simple and environmentally friendly process, high purity, and wide application range

Active Publication Date: 2021-06-15
上海珈凯生物股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no prior art for selectively extracting madecassoside using cyclodextrins

Method used

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  • Extraction method of centella asiatica extract with high biological safety
  • Extraction method of centella asiatica extract with high biological safety

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1, extracting high-purity madecassoside.

[0031] (1) Take the whole plant of Centella asiatica (produced in Yibin City, Sichuan Province, the same below), powder it and pass it through a 10-mesh sieve. Discard the powder whose particle size is too large to pass through the sieve, weigh 100 grams of the sieved centella asiatica powder and place it in a 2-liter round-bottomed flask, add 1 kilogram of ethyl acetate, soak at 40 °C for 1.5 hours, and filter to remove acetic acid ethyl ester.

[0032] (2) The cyclodextrin used in this embodiment is 2-hydroxypropyl-β-cyclodextrin, with a molecular weight of 1541.54; the molecular formula is, C 42 h 63 o 35 (C 3 h 7 O) 7 . Accurately weigh an appropriate amount of cyclodextrin and hydrogen peroxide, and dissolve them in water to obtain an aqueous solution with 10% by weight of hydrogen peroxide and 2% by weight of cyclodextrin. Fully mix the Centella asiatica powder treated with ethyl acetate in step (1) with 1...

Embodiment 2

[0036] Example 2, extracting high-purity madecassoside.

[0037](1) Weigh 120 grams of Centella asiatica whole herb, grind it and pass it through a 12-mesh sieve. Weigh 100 g of the sieved centella asiatica powder and place it in a 2L round bottom flask, add 1 kg of ethyl acetate, soak at 45°C for 1 hour, and filter to remove the ethyl acetate.

[0038] (2) The centella asiatica powder treated with ethyl acetate was thoroughly mixed with 1500 ml of an aqueous solution containing hydrogen peroxide and 2-hydroxypropyl-β-cyclodextrin. The weight percentage of hydrogen peroxide in the aqueous solution is 15%, and the weight percentage of 2-hydroxypropyl-β-cyclodextrin is 5%. Heating in a water bath, the temperature of the water bath does not exceed 80°C, the stirring speed is 120 rpm, and the extraction time is 1 hour; Step Extract again, and filter the extract. Combine the two extracts, add 60 g of 2-hydroxypropyl-β-cyclodextrin, stir at 50° C. for 3 hours, and stir at a rate ...

experiment example 1

[0063] Experimental example 1, purity analysis.

[0064] The purity of the madecassoside product obtained in the above examples and comparative examples was determined by HPLC analysis. The chromatographic conditions of the HPLC analysis are the same as the content determination method under the item of Centella asiatica in the 2020 edition of the Chinese Pharmacopoeia (page 296). The analysis results are shown in Table 1.

[0065] Table 1 embodiment 1,2 and comparative example 1,2,3 analysis result

[0066]

[0067] According to the results of the above examples and comparative examples, it can be seen that the content of madecassoside in the madecassoside sample prepared by the technical scheme of the present invention is about 90%, and the content of madecassoside and asiaticoside B is relatively low , and does not contain asiatic acid and madecassic acid. The technical solution of the present invention has remarkable progress.

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Abstract

The invention belongs to the technical field of natural active substance extraction, and particularly relates to an extraction method of a centella asiatica extract with high biological safety. The extraction method comprises the steps of degreasing, decoloring, cyclodextrin inclusion, removing asiaticoside and asiaticoside B, hydroxylating of asiaticoside and the like. The extraction method provided by the invention is simple and environment-friendly in process, high in product yield, light in color, high in biological safety and wide in application range.

Description

technical field [0001] The invention belongs to the technical field of bioactive substance extraction, and specifically refers to a method for extracting centella asiatica extract with higher biological safety. Background technique [0002] Centella asiatica (L.) Urban] is the whole plant of the genus Centella asiatica in the Umbelliferae family, a perennial creeping herb, mainly distributed in the south of the Yangtze River Basin, also known as Bengdawan, Luodeda, Callagrass, Lianxiancao, Banbianqian, etc. are commonly used traditional Chinese medicines recorded in the Chinese Pharmacopoeia. "Shen Nong's Materia Medica" records that it is cold in nature, bitter in taste, and pungent; it has the effects of clearing heat and dampness, detoxifying and reducing swelling, promoting blood circulation and removing blood stasis, and diuresis. Clinically, it is mostly used in surgery, dermatology, infectious hepatitis, and epidemic cerebrospinal meningitis. [0003] Centella asiat...

Claims

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Application Information

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IPC IPC(8): A61K36/23C07J63/00
CPCA61K36/23C07J63/008A61K2236/15A61K2236/333A61K2236/53A61K2236/51
Inventor 田军刘青高雅卢伊娜
Owner 上海珈凯生物股份有限公司
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