An endophytic Bacillus flexus tolerant to salt-alkali hydrolysis of silicon, phosphorus and potassium and its application
A Campylobacter, salinity-tolerant technology, applied in the application, bacteria, biocide and other directions, can solve the problem of little screening and research on the endophytic Bacillus salina, and improve the salt-tolerance ability and advantages of crops. Significantly improve the efficiency of fertilizer utilization
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Embodiment 1
0.02g, FeSO
4
0.02g, agar 20.0g, distilled water 1000mL), cultivated at 30°C for 3-7 days to obtain the present invention
At the same time, it has the strains that dissolve silicon circle, potassium circle and inorganic phosphorus circle.
4) Purification
Pick the bacterial strain that obtains in above-mentioned steps and has solution silicon circle, solution potassium circle, solution inorganic phosphorus circle simultaneously, receive by 5g / L
On a solid medium consisting of beef extract, 10g / L peptone, 50g / L sodium chloride, and 20g / L agar, wait for a single colony to grow to 2mm
, pick a single colony for use.
5) bacterial strain identification
Adopt Gram staining, observe thalline form under optical microscope to identify isolated bacterial strain, and its result is as follows:
[0063] Morphology and physiological and biochemical characteristics of the bacterial strain LXBF.1: the bacterial strain is a gram-positive, spore-producing irregular rod-shaped.
T...
Embodiment 2
Embodiment 3
[0073]
[0075]
[0077]
PUM
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