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An endophytic Bacillus flexus tolerant to salt-alkali hydrolysis of silicon, phosphorus and potassium and its application

A Campylobacter, salinity-tolerant technology, applied in the application, bacteria, biocide and other directions, can solve the problem of little screening and research on the endophytic Bacillus salina, and improve the salt-tolerance ability and advantages of crops. Significantly improve the efficiency of fertilizer utilization

Active Publication Date: 2022-06-03
领先生物农业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] It has been reported in the prior art that the peanut rhizosphere growth-promoting bacterium Bacillus flexus has the ability to decompose potassium and organophosphate; the endophytic bacterium Bacillus flexus PE4941 of Limonium has salt tolerance, but it has the characteristics of salt and alkali tolerance and the ability to decompose silicon, phosphorus and potassium. The ability of endophytic Bacillus flexus in Suaeda salsa to be screened and rarely studied

Method used

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  • An endophytic Bacillus flexus tolerant to salt-alkali hydrolysis of silicon, phosphorus and potassium and its application
  • An endophytic Bacillus flexus tolerant to salt-alkali hydrolysis of silicon, phosphorus and potassium and its application
  • An endophytic Bacillus flexus tolerant to salt-alkali hydrolysis of silicon, phosphorus and potassium and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

0.02g, FeSO

4

0.02g, agar 20.0g, distilled water 1000mL), cultivated at 30°C for 3-7 days to obtain the present invention

At the same time, it has the strains that dissolve silicon circle, potassium circle and inorganic phosphorus circle.

4) Purification

Pick the bacterial strain that obtains in above-mentioned steps and has solution silicon circle, solution potassium circle, solution inorganic phosphorus circle simultaneously, receive by 5g / L

On a solid medium consisting of beef extract, 10g / L peptone, 50g / L sodium chloride, and 20g / L agar, wait for a single colony to grow to 2mm

, pick a single colony for use.

5) bacterial strain identification

Adopt Gram staining, observe thalline form under optical microscope to identify isolated bacterial strain, and its result is as follows:

[0063] Morphology and physiological and biochemical characteristics of the bacterial strain LXBF.1: the bacterial strain is a gram-positive, spore-producing irregular rod-shaped.

T...

Embodiment 2

Embodiment 3

[0073]

[0075]

[0077]

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PUM

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Abstract

The present invention provides a strain of endogenous Bacillus flexus LXBF.1 resistant to salt-alkali hydrolysis of silicon, phosphorus and potassium and its application. The strain is named as Bacillus flexus LXBF.1 and was deposited in The General Microbiology Center of China Committee for Culture Collection of Microorganisms, the preservation number is CGMCC No.21705, and the 16S rDNA gene sequence of the strain is shown in SEQ ID NO:1. The bacterial strain of the present invention can convert insoluble inorganic phosphorus and silicon potassium into high-quality phosphorus, silicon and potassium element compounds that can be directly absorbed and utilized by plants, improve the bioavailability of insoluble phosphorus and silicon potassium in soil, and improve the utilization rate of fertilizers; Strong ability to produce IAA and siderophore can significantly improve the salt tolerance of crops, and has a very significant effect in promoting root growth and development, increasing crop yield, and improving quality; it has significant advantages in saline-alkali soil tolerance and strong colonization ability , is a major innovation in saline-alkali soil improvement and crop planting, and has broad application prospects.

Description

An endophytic Bacillus campylobacter tolerant to salt-alkali hydrolysis of silicophosphate and potassium and its application technical field The present invention relates to microbial fertilizer and saline-alkali soil crop adversity protection research technical field, especially relate to a strain Campylobacter endophytes resistant to salt-alkali hydrolysis of silicophosphorus potassium and its application. Background technique Saline-alkali soil refers to a series of soils subjected to saline-alkali stress, including various saline soils, alkaline soils and different degrees of salinization and Alkaline soil, saline-alkali soil has poor physical and chemical properties, high salt content, sticky soil, poor permeability, large volume, easy to harden, and small voids. The growth of most crops is inhibited to varying degrees, which seriously affects their yield and quality. Soil salinization is a serious type of land degradation, which is an important main reason fo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02A01N63/22A01P21/00C05F11/08C05G3/00C05G3/80C12R1/07
CPCC12N1/20C12N1/02A01N63/22C05F11/08C05G3/00C05G3/80Y02A40/10
Inventor 李丽艳杨娜葛振宇揣峻峰杜迎辉刘洪钧徐志文
Owner 领先生物农业股份有限公司
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