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Bifidobacterium bifidum for relieving constipation and regulating intestinal flora disorder and application thereof

A technology for bifidobacteria bifidum and constipation, applied in the field of microorganisms, can solve problems such as insignificant curative effect, and achieve the effects of improving diversity, restoring health, and improving the advancement rate of the small intestine.

Active Publication Date: 2021-06-18
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, Wang Junwen et al. pointed out in "Research Progress in the Treatment of Functional Constipation and Constipation-Predominant Irritable Bowel Syndrome" that bifidobacteria have no obvious curative effect on chronic constipation.

Method used

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  • Bifidobacterium bifidum for relieving constipation and regulating intestinal flora disorder and application thereof
  • Bifidobacterium bifidum for relieving constipation and regulating intestinal flora disorder and application thereof
  • Bifidobacterium bifidum for relieving constipation and regulating intestinal flora disorder and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0113] Embodiment 1: the acquisition of Bifidobacterium bifidum (Bifidobacterium bifidum) CCFM1166

[0114] Specific steps are as follows:

[0115] 1. Isolation and screening of bifidobacteria strains:

[0116] (1) Use a disposable aseptic toilet to collect the feces samples of male infants who were naturally delivered and breastfed in Suzhou City, Jiangsu Province, and the feces samples were mixed in a liquid containing MRS+mass percentage (0.05%-0.1%) cysteine ​​of fructooligosaccharides In the culture medium, enrich in the anaerobic incubator (N2:CO2:H2=80:10:10) for 12h;

[0117] (2) The feces samples were serially diluted with sterile saline, and then spread on the MRS+ mass percentage (0.05%-0.1%) L- On the solid plate of cysteine ​​hydrochloride, cultivate for 24-48h;

[0118] (3) select a single colony conforming to the basic form of bifidobacteria to carry out plate streaking and purification, and screen and isolate the selected strain of bifidobacteria;

[0119] ...

Embodiment 2

[0138] Example 2: Bifidobacterium bifidum (Bifidobacterium bifidum) CCFM1166 relieves constipation-related symptoms induced by loperamide

[0139] Specific steps are as follows:

[0140] (1) Preparation of Bifidobacterium bifidum CCFM1166 bacterial suspension

[0141] Bifidobacterium bifidum CCFM1166 was taken out from the -80°C refrigerator, streaked in the MRS solid medium, cultured at 37°C for 48 hours, picked a single colony in the MRS liquid medium, and cultured at 37°C for 24 hours to prepare the seeds liquid;

[0142] The prepared seed solution was inoculated in a new MRS liquid medium with an inoculum size of 2% (v / v), cultivated at 37°C for 24h, and cultured for another generation in the same way to prepare Bifidobacterium bifidum CCFM1166 fermentation liquid;

[0143] Then the prepared Bifidobacterium bifidum CCFM1166 fermentation broth was centrifuged at 6000r / min and 4°C for 5min, and then resuspended with 10% (mass volume ratio w / v) skim milk to obtain a bacter...

Embodiment 3

[0156] Embodiment 3: Bifidobacterium bifidum (Bifidobacterium bifidum) CCFM1166 can significantly reduce the content of somatostatin (SS) in the serum of constipation mice

[0157] Specific steps are as follows:

[0158] (1) C57BL / 6J mice grouping, modeling and treatment methods are the same as in Example 2.

[0159] (2) After the mice were killed on the 40th day, the collected mouse blood was left to stand for 2 hours, and the serum was obtained after centrifugation at 3000×g for 15 minutes. The somatostatin (SS) detection kit was used to perform experiments according to the instructions, and the standard curve Calculate the concentration of SS in serum.

[0160] Studies have shown that somatostatin, as a neurohormone with a wide range of effects, can inhibit the release of various gastrointestinal hormones, such as gastrin, motilin, glucagon and calcitonin. Therefore, it has a certain inhibitory effect on gastrointestinal motility.

[0161] Experimental results such as ...

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Abstract

The invention discloses a bifidobacterium bifidum for relieving constipation and regulating intestinal flora disorder and application thereof, and belongs to the field of microorganisms. The bifidobacterium bifidum provided by the invention can obviously improve the water content of excrement and the intestinal propulsive rate and reduce the first black excrement time of constipation mice, and has the effect of obviously improving apparent pathological indexes of constipation; in the detection of physiological indexes, the bifidobacterium bifidum can also improve the content of motilin and reduce the content of somatostatin, so that the intestinal tract movement is fundamentally influenced; the bifidobacterium bifidum can regulate the abundance of important microorganisms in the intestinal tract and maintain the steady state of the intestinal tract. Compared with GDMCC NO: 60940, the bifidobacterium bifidum CCFM1166 has the advantages that the content of MTL in serum is better increased, the content of SS in the serum is reduced, and the promotion effect on intestinal peristalsis is more remarkable, so that a more personalized treatment scheme can be adopted for constipation patients in the future, and the application prospect is very considerable.

Description

technical field [0001] The invention relates to a strain of bifidobacterium bifidum capable of alleviating constipation and regulating intestinal flora disorder and application thereof, belonging to the field of microorganisms. Background technique [0002] As we all know, constipation is a kind of global disease with a very high prevalence rate and a wide range of influence. At present, constipation has appeared in all age groups around the world, causing many people to suffer. Therefore, for Constipation-related treatments need more attention and attention. [0003] There are many causes of constipation, such as disturbance of daily work and rest, unbalanced diet, side effects of some drugs, and even complications of some diseases. These different causes may also bring about different symptoms of constipation. Therefore, constipation is distinguished according to different classification methods. According to the mode of onset, it can be divided into acute constipation a...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23C9/123A23C13/16A23C19/032A23C19/06A23L11/50A23L11/65A23L33/135A61K35/741A61P1/10A61P1/00C12R1/01
CPCA23C9/1234A23C13/16A23C19/0323A23C19/062A23C11/106A23L33/135A61K35/741A61P1/10A61P1/00A23V2002/00A23V2400/517A23V2200/3204
Inventor 王琳琳王刚柴茂赵建新陈卫张灏
Owner JIANGNAN UNIV
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