Biologically active composition and its preparation method and application
A technology of biological activity and composition, which is applied in the field of biological active composition and preparation, can solve the problems of poor removal effect of metal ions, and achieve the effects of improving damage repair effect, removing heavy metals by adsorption, promoting migration and proliferation
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[0057] The preparation method of the stem cell secretion provided by the present invention is induced by combining drugs and X-rays in the preparation process, so that the SDF-1 chemokine in the stem cell secretion can be expressed at a high level, and can better induce endogenous stem cells in wounds. Regional enrichment improves the effect of bone damage repair.
[0058] In a preferred embodiment of the present invention, when the mass concentration of iohexol is 2-100 μg / mL, it is easy to co-culture with umbilical cord stem cells, especially when the mass concentration of iohexol is 10-20 μg / mL, Easier to co-culture with umbilical cord stem cells.
[0059] Typical but not limiting, the mass concentration of iohexol used for co-culture with umbilical cord stem cells is 2, 5, 8, 10, 20, 50, 80 or 100 μg / mL.
[0060] In a preferred version of the present invention, when the X-ray irradiation intensity applied simultaneously for drug induction is 0.5-4Gy, the expression level ...
Embodiment 1
[0105] This embodiment provides a stem cell secretion, which is obtained by enrichment according to the following steps:
[0106] (1) Obtain umbilical cord stem cells: take the umbilical cord out of the sterile container and place it in a disposable plate, use surgical scissors to cut the umbilical cord into about 2cm / section; wash the blood with normal saline containing heparin sodium, cut the umbilical cord longitudinally, Remove 3 blood vessels and skin, wash the blood inside; cut the umbilical cord tissue into 2.0-4.0mm 3 For small pieces, wash them with normal saline without heparin, and drain the water. Add a drop of DMEM / F12 + 10% fetal bovine serum medium containing penicillin and streptomycin to moisten the above tissue pieces, and store at 37°C, 5% CO 2 Incubate in a static incubator for 0.5-1h. Then, add the above-mentioned double-antibody-containing medium until the skin tissue block is covered, and place in 5% CO 2 , 37°C, and cultured in an incubator with satu...
Embodiment 2
[0111] This embodiment provides a stem cell secretion, the difference between its preparation method and embodiment 1 is that in step (3), 2Gy of X-ray irradiation is applied, and the remaining steps and process parameters are the same as in embodiment 1. This will not be repeated here.
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