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Lipid carbon-carbon double bond isomer mass spectrum imaging method based on visible light catalysis, and application thereof

A mass spectrometry imaging and double bond isomerization technology, applied in the field of mass spectrometry detection, can solve the problems of very demanding reaction conditions, easy to be affected by the external environment, and damage to the health of the experimenter, and achieve simple and good practical application of derivatization reagents. The value of the reaction, the effect of mild reaction conditions

Active Publication Date: 2021-07-02
SHANDONG ANALYSIS & TEST CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the inventors found that directly performing the Paternò-Büchi (PB) derivatization reaction on the tissue section is easily affected by the external environment, and the requirements for the reaction conditions are very harsh.
In addition, the Paternò-Büchi (PB) derivatization reaction needs to be carried out under ultraviolet light irradiation, and ultraviolet light irradiation is not only likely to cause side reactions, but may also cause health damage to the experimenter

Method used

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  • Lipid carbon-carbon double bond isomer mass spectrum imaging method based on visible light catalysis, and application thereof
  • Lipid carbon-carbon double bond isomer mass spectrum imaging method based on visible light catalysis, and application thereof
  • Lipid carbon-carbon double bond isomer mass spectrum imaging method based on visible light catalysis, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1: Visible light catalytic derivatization mass spectrometry imaging analysis of fatty acid C=C double bond isomers on a glass slide

[0053] (1) Accurately weigh 10 mg of 6Z-fatty acid (FA)-C18:1, 9Z-FA-C18:1, and 11Z-FA-C18:1 reference substances respectively in a 10 mL volumetric flask, add 10 mL of acetonitrile:water ( 50:50, v / v) solution, vortexed, ultrasonicated for 3 minutes to obtain 6Z-FA-C18:1, 9Z-FA-C18:1, 11Z-FA-C18:1 solutions;

[0054] (2) Pass the above-mentioned different fatty acid C=C double bond isomer solutions through HTX TM-Sprayer respectively TM The spraying instrument is sprayed on the ITO-indium tin oxide conductive glass slide, and the density of the fatty acid reference substance on the ITO-indium tin oxide conductive glass slide is 4.7nmol / cm 2 ;

[0055] (3) Accurately weigh 18.3mg of 3-benzoylpyridine and place it in a 10mL volumetric flask, add 10mL of acetonitrile:water (50:50, v / v) solution, vortex and mix, and ultrasonicate f...

Embodiment 2

[0063] Example 2: Derivatized mass spectrometry imaging analysis of two double bond position isomers of phosphatidylcholine (PC)-C34:1 in rat brain

[0064] (1) Take fresh rat brain tissue, and use the CryoStar microtome of Thermo Electric Company to make frozen slices of brain tissue with a thickness of 12 μm in the coronal and horizontal planes;

[0065] (2) The coronal and horizontal frozen sections of the brain tissue were transferred to an ITO-indium tin oxide conductive glass slide, and vacuum-dried for 15 minutes.

[0066] (3) Accurately weigh 18.3mg of 3-benzoylpyridine and place it in a 10mL volumetric flask, add 10mL of acetonitrile:water (50:50, v / v) solution, vortex and mix, and ultrasonicate for 3 minutes to obtain 10mmol / L of 3-benzoylpyridine solution;

[0067] (4) Pass the 3-benzoylpyridine solution through the HTX TM-Sprayer TM The spraying instrument is sprayed onto the coronal and horizontal slices of brain tissue, and the spraying density of 3-benzoylpyr...

Embodiment 3

[0075] Example 3: Derivatization mass spectrometry imaging analysis of two double bond position isomers of phosphatidylcholine (PC)-C36:1 in rat brain

[0076] (1) Take fresh rat brain tissue, and use the CryoStar microtome of Thermo Electric Company to make frozen slices of brain tissue with a thickness of 12 μm in the coronal and horizontal planes;

[0077] (2) The coronal and horizontal frozen sections of the brain tissue were transferred to an ITO-indium tin oxide conductive glass slide, and vacuum-dried for 15 minutes.

[0078] (3) Accurately weigh 18.3mg of 3-benzoylpyridine and place it in a 10mL volumetric flask, add 10mL of acetonitrile:water (50:50, v / v) solution, vortex and mix, and ultrasonicate for 3 minutes to obtain 10mmol / L of 3-benzoylpyridine solution;

[0079] (4) Pass the 3-benzoylpyridine solution through the HTX TM-Sprayer TM The spraying instrument is sprayed onto the coronal and horizontal slices of brain tissue, and the spraying density of 3-benzoyl...

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Abstract

The invention provides a lipid carbon-carbon double bond isomer mass spectrum imaging method based on visible light catalysis, and application thereof, and belongs to the technical field of mass spectrum detection. A novel derivatization reagent is sprayed on the surface of a tissue slice, then a tissue in-situ derivatization reaction is carried out under the catalysis of visible light, and mass spectrum imaging analysis is further carried out on the derivatized tissue slice by adopting an MALDI mass spectrum imaging technology, so that in-situ visual characterization of a lipid C=C double-bond isomer in a biological tissue is realized, therefore, the method has good practical popularization and application values.

Description

technical field [0001] The invention belongs to the technical field of mass spectrometry detection, and in particular relates to a mass spectrometry imaging method of lipid carbon-carbon double bond isomers based on visible light catalysis and an application thereof. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Lipid components such as fatty acids and phospholipids play an indispensable role in building cell biomembranes, transmitting biological signals, and regulating cellular energy metabolism. More and more evidence shows that the change of lipid composition in the body is closely related to the occurrence and development of various diseases such as tumors, Alzheime...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/64G01N1/28
CPCG01N27/64G01N1/28
Inventor 孙成龙王晓马春霞李丽丽王岱杰
Owner SHANDONG ANALYSIS & TEST CENT
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