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Method for cultivating non-heading Chinese cabbage microspore plants

A technology of heading Chinese cabbage and microspores, which is applied in the field of cultivating non-heading Chinese cabbage microspores, can solve the problems of pollution, cumbersome operation, and the rate of embryo emergence being affected by genotype, so as to improve the embryo emergence rate, simplify operation steps, The effect of shortening the test time

Inactive Publication Date: 2021-07-09
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention is mainly aimed at improving the problems of cumbersome operation of the current technology, the influence of genotype on the germination rate and pollution, etc., thereby providing an efficient and simple method for cultivating microspore plants of non-heading Chinese cabbage

Method used

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  • Method for cultivating non-heading Chinese cabbage microspore plants
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  • Method for cultivating non-heading Chinese cabbage microspore plants

Examples

Experimental program
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Effect test

Embodiment 1

[0044] The cultivation of the microspores of non-heading cabbage of embodiment 1 different varieties

[0045] Select 8 different varieties (ideal big tree Suzhou green, white-stalked chicken feathers, I112, hot fast vegetables, I159, X-2, fast vegetables, Qinggengcai) to carry out microspore culture with the following method, and count the embryonic rate:

[0046] 1. Flower bud selection: During the blooming period of non-heading cabbage, choose a sunny morning, and select plants with good growth and no pests and diseases around 10 o'clock. Use tweezers to select the inflorescences of the plants and put them in a plastic bag. Add a small amount of sterile Keep the flower buds moist with water, and put them in a 4°C refrigerator for low-temperature pretreatment for 24 hours.

[0047]Use tweezers to select flower buds of different non-heading cabbage varieties from the pretreated inflorescences. The flower buds should be full, free from diseases and insect pests, and the length ...

Embodiment 2

[0058] Example 2: Effects of Different Concentrations of MT Treatment on Microspore Germination Rate of Non-heading Chinese Cabbage

[0059] Select the difficult-to-embryo variety 'I159', and the easy-to-embryo varieties 'Quaicai' and 'Qinggencai' are cultivated according to the method in Example 1, wherein in the step of "microspore dilution and subpackaging", the samples of each variety are divided into Group 5, in addition to adding activated carbon solution, also added melatonin (Melatonin, MT) for treatment, so that the concentration of melatonin in the mixed solution was 0 mg / L (CK), 0.025 mg / L, 0.05 mg / L, 0.1 mg / L, 0.2mg / L, such as Figure 8 As shown, the embryonic rate of microspores of the three varieties showed a trend of first increasing and then decreasing with the increase of MT concentration. When 'I159' was not treated with MT, no embryoid bodies were produced, but after treatment with different concentrations of MT, a small amount of embryoid bodies could be p...

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Abstract

The invention discloses a method for cultivating non-heading Chinese cabbage microspore plants. The method for cultivating the non-heading Chinese cabbage microspore plants comprises the following steps of (1) sampling and selecting flower buds; (2) carrying out microspore cultivation, specifically, disinfecting the flower buds with sodium hypochlorite, cleaning the flower buds twice with sterilized water, and rinsing the flower buds with a 1 / 2NLN-13 culture medium; grinding, filtering and centrifuging the rinsed flower buds, diluting yellow-green precipitates with an NLN-13 culture medium, adding activated carbon, adding melatonin, sub-packaging the yellow-green precipitates into 35mm culture dishes, carrying out heat shock at 33 DEG C for 24 hours, and carrying out dark culture for 10 days to form embryoids; and (3) carrying out plant regeneration, specifically, carrying out light-supplementing shaking culture on the embryoids, then transferring the embryoids into an improved solid 1 / 2MS culture medium to be cultured for 50 days, and carrying out acclimatization and transplantation. According to the method for cultivating the non-heading Chinese cabbage microspore plants a part of varieties difficult to germinate can be induced to germinate, the germination rate of varieties easy to germinate is improved, the operation steps are simplified, the test time is shortened, and the survival rate of regenerated plants is improved.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to a method for cultivating non-heading Chinese cabbage microspore plants. Background technique [0002] Non-heading Chinese cabbage (Brassica campestris ssp.chinensis Makino), often called Chinese cabbage, belongs to the genus Brassica of the family Cruciferae (Cruciferae). It has good taste, high edible value, short growth cycle and strong adaptability. It is planted everywhere and is one of the important vegetable crops in my country. Non-heading Chinese cabbage is a frequently cross-pollinated crop. It is one of the common breeding methods to obtain heterosis lines by crossing superior parents. Therefore, how to obtain stable homozygous parents is of great significance for the germplasm innovation of non-heading Chinese cabbage. [0003] Isolated Microspore Culture (IMC) refers to the process of isolating microspores by mechanically squeezing flower buds, and cultiv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 李英蒋林钫侯喜林王建军
Owner NANJING AGRICULTURAL UNIVERSITY
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