Anti-cd5 protein monoclonal antibody and its cell line, preparation method and application

A monoclonal antibody and protein technology, applied in the field of biomedical engineering, can solve problems such as unclear mechanism and achieve high specificity
CN113105547BActive Publication Date: 2022-04-08FUZHOU MAIXIN BIOTECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
FUZHOU MAIXIN BIOTECH CO LTD
Publication Date
2022-04-08

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Abstract

The invention relates to a monoclonal antibody capable of recognizing human CD5 antigen, a secreting cell line, its preparation method and its application in immunoassay. The above technical scheme selects the 398th to 495th amino acid at the C-terminus of the CD5 protein as the antigenic peptide, and performs codon optimization to become a gene fragment suitable for expression in Escherichia coli BL21. The resulting recombinant protein contains the CD5 protein fragment and histidine protein tag. The recombinant protein is used to immunize mice, and through cell fusion, screening and subcloning, the mouse hybridoma cell line 11H3 that efficiently secretes anti-CD5 protein monoclonal antibody, and the anti-CD5 protein monoclonal antibody secreted by the cell line are obtained Antibody. The antibody obtained by this protocol has high specificity and sensitivity, can specifically recognize cells expressing CD5 protein, and is suitable for immunological detection, especially immunohistochemical detection.
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Description

technical field

[0001] The invention relates to the field of biomedical engineering, in particular to an anti-CD5 protein monoclonal antibody and its cell line, preparation method and application. Background technique

[0002] The human CD5 gene is located on chromosome 11q12.2. The CD5 molecule is a 67KD transmembrane glycoprotein expressed in a subclass of T cells and virgin B cells. CD5 is a macrophage scavenger receptor rich in cysteine (SRCR) Domain homology domain receptor family members, these CD5-positive B cells can produce autoantibodies, and can also spread in autoimmune diseases and lymphoid malignancies, such as leukemia, MCL and CD5-positive The DLBCL. Unlike mouse B1 cells that express CD5, human B1 cells do not have CD5 expression. Experiments in chicken egg lysozyme transgenic models in mice and chicken CD5-knockout cells showed that CD5 expression promotes tolerance to self-reactive antigens in vitro, while the absence of CD5 molecules leads to enhanced c...

Claims

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