Method for detecting effective components of traditional Chinese medicine composition for treating kidney diseases

A detection method and composition technology, applied in the direction of measuring devices, material separation, and analysis of materials, can solve the problems of expensive reagents, long detection cycle, and complicated pretreatment procedures, so as to improve accuracy and reproducibility, save Pretreatment time, good separation effect

Active Publication Date: 2021-07-16
云南雷允上理想药业有限公司
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  • Claims
  • Application Information

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Problems solved by technology

In order to eliminate interference, the pretreatment procedures of samples are complex and cumbersome, and a large amount of organic reagents are required to be purified repeatedly, which is laborious, time-consuming, wastes reagents, pollutes the environment, endangers health, and has a long detecti

Method used

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  • Method for detecting effective components of traditional Chinese medicine composition for treating kidney diseases
  • Method for detecting effective components of traditional Chinese medicine composition for treating kidney diseases
  • Method for detecting effective components of traditional Chinese medicine composition for treating kidney diseases

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0108] 1.1 Instruments and reagents

[0109] Balance: OHAUS AR224-N electronic balance (1 / 10,000), EX125ZH electronic analytical balance (1 / 100,000), Ohaus Instruments (Changzhou) Co., Ltd.;

[0110] Instrument: DK-98ⅡA Electric Heating Constant Temperature Water Bath, Tianjin Test Instrument Co., Ltd.;

[0111] Ultrasonic instrument: SB25-12DTD ultrasonic instrument, Ningbo Xinzhi Biotechnology Co., Ltd.;

[0112] Ultraviolet observer: TU-II, Shanghai Kezhe Biochemical Technology Co., Ltd.;

[0113] Thin layer board: Qingdao ocean silica gel board, Jiyida silica gel board, Bangkai silica gel board, Huanghai silica gel board.

[0114] Coptis rhizome reference medicinal material, China National Institutes for Food and Drug Control, batch number 120913-201611.

[0115] Achyranthes bidentata reference medicinal material, China Institute for Food and Drug Control, batch number 121066-201809.

[0116] Chenpi reference medicinal material, China National Institutes for Food and D...

Embodiment 2

[0200] 2.1 Achyranthes bidentata TLC identification

[0201] A number of extraction methods and development systems were discussed, and after identification with ecdysterone and oleanolic acid as reference substances, it was finally determined that the silica gel G plate was used as the development plate, and dichloromethane-methanol-formic acid (10:2: 0.05) is the expansion system, ecdysterone is the reference substance, supplemented with Achyranthes bidentata reference medicinal material, 10% ethanol sulfate, a set of Achyranthes bidentata TLC identification method (see Figures 34 to 47 ).

[0202] 2.1.1

[0203] The test solution of Achyranthes bidentata prepared by the method in 1.2.1.

[0204] The reference substance solution takes the reference substance solution prepared by the method in 1.3.1.

[0205] According to the experiment of thin-layer chromatography ("Chinese Pharmacopoeia" 2015 edition four general rules 0502), draw 10 μl of the test solution, 10 μl of th...

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Abstract

The invention relates to a method for detecting effective components of a traditional Chinese medicine composition for treating kidney diseases, which comprises the step of detecting the effective components of coptis chinensis, salvia miltiorrhiza, pericarpium citri reticulatae and/or achyranthes bidentata, such as berberine hydrochloride, ecdysterone, hesperidin and/or protocatechualdehyde by adopting thin-layer chromatography. The thin-layer chromatography provided by the invention can qualitatively detect the effective components of the traditional Chinese medicine composition for treating kidney diseases, is simple and convenient to operate, good in repeatability and stability and accurate in result, and enhances the rationality and reliability of the whole effective component detection method as a qualitative detection method for the effective components of the traditional Chinese medicine compound preparation.

Description

technical field [0001] The invention relates to the field of drug detection, in particular to a method for detecting active ingredients of a traditional Chinese medicine composition for treating nephropathy. Background technique [0002] Kidney diseases such as renal failure are common serious diseases in clinical practice, and are usually treated with dialysis and kidney transplantation. However, the medical expenses are too high for general families to afford, and complications are likely to occur during dialysis treatment. Rejection is common after kidney transplantation, which is generally difficult for patients and their families to bear. The application of traditional Chinese medicine such as Shenshuaining Capsules can effectively avoid the occurrence of the above situation, which can effectively relieve symptoms and reduce the pain of patients, but in order to ensure a more significant clinical effect, it needs to be improved. [0003] In order to ensure that high-qu...

Claims

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Application Information

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IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 罗红艳朱雄覃思光李垚刘宗耀黄丽桃胡德美
Owner 云南雷允上理想药业有限公司
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