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Monascus purpureus W-4 with high yield of lovastatin and application thereof

A purple monascus and monascus technology, which is applied to violet monascus W-4 and its application field to achieve the effects of improving color and luster, strengthening lipid-lowering health care functions, and increasing safety

Active Publication Date: 2021-07-23
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on mutation breeding for the purpose of simultaneously increasing the production of lovastatin and monascus pigment. ARTP mutagenesis technology has been used to select and breed functional Monascus strains with stable and high lovastatin production and high monascus pigment content. It is of great significance to discover the resources of Monascus strains and their production and application

Method used

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  • Monascus purpureus W-4 with high yield of lovastatin and application thereof
  • Monascus purpureus W-4 with high yield of lovastatin and application thereof
  • Monascus purpureus W-4 with high yield of lovastatin and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Alternative plasma plasma mutagenesis and screening

[0027] (1) Preparation of spore fluid from strains:

[0028] The original red scholastin was seeded on the PDA medium. After 7 days of 28 ° C, the fungi and spores were scraped with a vaccination shovel, transferred to a triangular bottle containing 30 ml of sterile water, and the triangular bottle is covered with small glass beads. 180R / min oscillated 20 min, fully taking a spore after filtration, and a spore suspension was prepared. The final dilution adjustment makes the spore concentration of 10 6 A / ml suspension.

[0029] (2) Determination of plasma mutagenicity:

[0030] Take 10 μL of a spore suspension in the ultra-clean workbench onto the surface of the slide, and the sterile wind is dried to form a bacterial film, and the pliers are placed in the mutager. In the case of 10 L / min, the plasma emission source and the sample distance are 2 mm, the operating temperature is 23.0 ~ 35.0 ° C, and the mu...

Embodiment 2

[0051] Example 2: Preparation of a red yeast starter

[0052] The preparation method of the red yeast starter was specifically: wash the purple red fixation spore on the inclination with 0.1% Sterile Tempe Water water, and transferred 100 g of suede to the tapered bottle after 2 h, and the seal was sealed at 121 ° C, 0.08 MPa After 20 min, the rice is scattered, inoculation 10 6 A spore / g (rice dry weight) is inoculated to cool to room temperature solid fermentation medium, and the amount of water in the sterile water regulation is 40% to 50%; after mixing, the medium is piled up to the bottle of the bottle, 28-30 After 24-48 hours of retained culture, it was scattered, and the flask was shaken for 1 to 2 times per 18-24H, until 14-18d fermentation, resulting in a red-curl fermentation containing red cumi W-4.

[0053] The above solid fermentation medium is a commercially available nip preparation.

[0054] The above potato glucose water medium formulation: potato (peeled) 200g,...

Embodiment 3

[0055] Example 3: Application of Crack Fermectors prepared by Purple Red Societium W-4 in Red Quarter

[0056] The red-curmes prepared by purple red bacteria were used for red-curmes, and the steamed rice (140%), activated yeast (10%), and wheat (8%), Red (8%), water (123%) and other mixed materials. Pre-fermentation of 28 ° C for 4d, opening the 耙 daily; after fermentation of 15 ° C for 20d, the opening is 2d. After the end of the fermentation, the original wine, 80 ° C water bath was sterilized for 30 min, and the sealed was sealed. Sampling detects alcoholic precision, pH, total acid, amino acid state nitrogen, lovastatin, color price, orange maternal indicators.

[0057] Eco-precision, pH, total acid, amino acid state measurement: Test by GB / T 13662-2008 "Yellow Wine".

[0058] Lovastatin, color price, orange matrixtocin are detected in the middle of Example 1.

[0059] After testing, alcoholic alcohol was 14.0% Vol, pH 4.29, total acid content 4.63 g / l, amino acid state n...

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Abstract

The invention discloses monascus purpureus W-4 with high yield of lovastatin and application thereof, and belongs to the technical field of fermentation engineering and biology. According to the monascus purpureus W-4, a strain of monascus purpureus W-4, which can produce the lovastatin at high yield and is free of citrinin, is bred by virtue of an atmospheric and room temperature plasma (atmospheric and room temperature plasma, ARTP) technology. The yield of the lovastatin in a monascus leavening agent prepared through solid state fermentation of monascus purpureus can reach 30-35mg / g, and the color value can reach 5000-6000mu / g. When the strain is applied to preparation of fermented foods such as yellow wine, vinegar, soy sauce, thick broad-bean sauce and bread, the content of the lovastatin is at a relatively high level, and no citrinin is detected.

Description

Technical field [0001] The present invention relates to a purple red fixation W-4 and its application thereof, which belongs to fermentation engineering and biotechnology. Background technique [0002] Red song is a great invention created by my country, which is a unique traditional brewed food that is fermented by rice as a major raw material. Not only rich natural red pigments, but also have medicinal health care, mainly used in the wine industry, the pharmaceutical industry and the food industry. Monascus, belonging to red curburous fungi, a variety of strains, and the ability to produce polyketone compounds in different strains is also large. The lovastatin and red pigment is its main metabolites, of which lovastatin is widely used in medical and functional foods due to significant fat-lowering efficacy; red laminin is a natural functional pigment, widely used in the food industry In particular, meat products are often used as food additives. But as a traditional fermented f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12G3/022C12G3/026C12J1/04A23L7/104A23L11/50A23L27/50A23L29/00A23L33/00A21D8/04C12R1/645
CPCC12N1/14C12G3/022C12G3/026C12J1/04A23L7/104A23L27/50A23L29/065A23L33/00A21D8/045A23V2002/00A23V2200/30
Inventor 毛健刘双平吴玉峰
Owner JIANGNAN UNIV
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