A lysinibacillus owin-1
A technology of lysine bacillus and bacillus, applied in the direction of bacteria, transportation and packaging, biochemical equipment and methods, etc., can solve the problems of poor stress resistance, high salt content, low efficiency, etc., achieve improved effect and good application effect , The effect of solving the difficulty of long-distance transportation
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Embodiment 1
[0015] Add corn flour and soybean flour to the sludge from the food waste treatment plant and stir evenly, add sterile water and then carry out bacterial value-added in a sterile environment.
[0016] After 72 hours, the mixed material was weighed, diluted with sterile water, and streaked and spread on the selection medium. The composition of the medium included yeast extract 10g, peptone 10g, agar 10g, glucose 10g, magnesium sulfate 1g, chloride Sodium 1g, calcium chloride 1g, potassium phosphate 30g.
[0017] Cultivate at 37°C for 24 hours; raise the temperature to 45°C and then cultivate at 24°C; complete the preliminary screening of colonies.
[0018] Select well-growing colonies and culture them on a shaker in a liquid medium containing 3g of beef extract, 10g of peptone, 5g of NaCl and 1000ml of water, and cultivate them at 37°C for 24 hours;
[0019] Add 5g of sodium chloride to the liquid medium, and continue to culture at 37°C, 200r / h shaker for 36 hours;
[0020] T...
Embodiment 2
[0029] 1. Take the amplified Bacillus lysinica Owin-1 obtained by separation and screening in Example 1 and the bacterial suspension of two commercially available bacteria for the treatment of kitchen waste, centrifuge, and resuspend the bacteria with 5% glucose solution respectively , to ensure that there are no other sources of nitrogen in the system. After adding a certain concentration of ammonia water and indole solution, place it at 37°C and shake at 220rpm on a shaker, and use the same volume of sterile system as a negative control. Samples are taken at certain time intervals, and the content of nitrogen-containing compounds in the supernatant obtained by centrifugation is determined by colorimetry. 5% glucose solution was used as blank control. Concentration-time curves (aging curves) of nitrogen-containing compounds were drawn, and the results were analyzed and compared.
[0030] ammonia
[0031] Reaction mechanism: Ammonium nitrogen reacts with sodium hypochlorite...
Embodiment 3
[0050] Use the Bacillus lysinica Owin-1 obtained by the isolation and screening of Example 1 and two kinds of commercially available food waste treatment bacteria to prepare liquid bacterial agents, which contain 50g of Na 2 HPO 4 ·7H 2 O, 30 g KH 2 PO 4 , 1g CaCl 2 ·6H 2 O, 5g of NaCl, 60g of glucose, 3g of sodium acetate, 1g of ZnSO 4 ·7H 2 O, 10 g NH 4 CI, 60g of Casamino Aci, 10L of distilled water H 2 O, and 1x10 10 cfu / ml live bacteria.
[0051] Weigh 30kg of kitchen waste and divide it into 3 parts, stir and mix them thoroughly, put them in the treatment tank, and add 150g of bacterial agent to each part. And turn it every 1 hour, and test the organic matter degradation rate of the kitchen waste every 6 hours. The test results are shown in Table 1.
[0052] Table 1: Organic matter degradation rate (%) of two groups of bacterial agents at different times
[0053] group 12 18 24 30 36 40 48 Owin-1 13.2 28.6 50.8 61.4 77.3 80.2 83.2...
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