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A kind of oily sludge degrading bacterial strain proteus mirabilis SB and its application

A technology of oily sludge and bacterial strains, applied in water/sludge/sewage treatment, bacteria, water pollutants, etc., can solve problems such as research limitations, achieve the effects of enhancing biological activity, optimizing soil microbial system, and promoting degradation

Active Publication Date: 2022-06-17
陕西大胜石油工程技术服务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Proteus mirabilis is a kind of Proteus genus, which often exists in human feces and is an opportunistic pathogen. There are many studies on this strain, but most of its sources are in animals or their feces. also mostly limited to biomedical aspects

Method used

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  • A kind of oily sludge degrading bacterial strain proteus mirabilis SB and its application
  • A kind of oily sludge degrading bacterial strain proteus mirabilis SB and its application
  • A kind of oily sludge degrading bacterial strain proteus mirabilis SB and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Degradation and remediation test of Proteus mirabilis SB on actual oily sludge in Yanchang Oilfield

[0037] Take oily sludge from Yanchang Oilfield, air-dry, grind, pass through a 2mm sieve, and set aside. The strain Proteus mirabilis SB was inoculated into the broth medium, and the Erlenmeyer flask was placed in a shaker for activation at 120rpm and 30°C for 24h. Weigh 20kg of the oily sludge after the above-mentioned air-drying, grinding and sieving, and inoculate the bacterial liquid in the oily sludge with an inoculation amount of 10wt%. In order to increase the air permeability of the oily sludge in the fermentation process, add 1kg to the oily sludge. At the same time, add 200g of ammonium chloride and 45g of potassium dihydrogen phosphate to make C:N:P=90:5:1, and control the moisture content to 20% and the temperature to 25℃. -80cm, stirring and supplementing water every 3 days, the treatment period is 60 days.

[0038] During the microbial treatment, samples...

Embodiment 2

[0044] Degradation and remediation test of Proteus mirabilis SB on oily sludge in Yanchang Oilfield

[0045] Take oily sludge from Yanchang Oilfield, air-dry, grind, pass through a 2mm sieve, and set aside. The strain Proteus mirabilis SB was inoculated into the broth medium, and the Erlenmeyer flask was placed in a shaker for activation at 120rpm and 30°C for 24h. Weigh 20kg of the oily sludge after the above-mentioned air-drying, grinding and sieving, and inoculate the bacterial liquid in the oily sludge with an inoculation amount of 5wt%. In order to increase the air permeability in the oily sludge fermentation process, add 1kg of Sawdust, add 200g of ammonium chloride and 45g of potassium dihydrogen phosphate at the same time to make C:N:P=90:5:1, and control the moisture content to 20% and the temperature to 25℃. After 60 days of biodegradation, the oily sludge was tested, and it was found that the degradation rate of total petroleum hydrocarbons (TPH) in the oily sludge...

Embodiment 3

[0047] Degradation and remediation test of Proteus mirabilis SB on oily sludge in Yanchang Oilfield

[0048] Take oily sludge from Yanchang Oilfield, air-dry, grind, pass through a 2mm sieve, and set aside. The strain Proteus mirabilis SB was inoculated into the broth medium, and the Erlenmeyer flask was placed in a shaker for activation at 120rpm and 30°C for 24h. Weigh 20kg of the above-mentioned oily sludge after air-drying, grinding and sieving, and inoculate the bacterial liquid in the oily sludge with an inoculation amount of 10wt%. In order to increase the air permeability in the oily sludge fermentation process, add 1kg of Sawdust, at the same time, add 200g of ammonium chloride and 45g of potassium dihydrogen phosphate to make C:N:P=90:5:1, and control the moisture content to 30% and the temperature to 25℃. After 60 days of biodegradation, the oily sludge was tested, and it was found that the degradation rate of total petroleum hydrocarbons (TPH) in the oily sludge w...

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Abstract

The invention provides an oily sludge degrading bacterial strain Proteus mirabilis SB and its application; the bacterial strain is Proteus mirabilis, which was preserved in the China General Microorganism Culture Collection and Management Center on January 25, 2021, and the preservation number is CGMCC NO.21729. The present invention separates and obtains Proteus mirabilis SB from oily sludge, so the bacterial strain quickly becomes the dominant bacterial group after entering the oily sludge, effectively promotes the degradation of petroleum hydrocarbons, improves the physical properties of the soil, and enhances the biological activity of the soil; The strain can metabolize biosurfactant during growth and reproduction, and increase the degradation rate of microorganisms to petroleum hydrocarbons in oil sludge. The bacterial strain Proteus mirabilis SB involved in the present invention degrades petroleum pollutants in oily sludge in a short period of time, and the degradation rate of total petroleum hydrocarbons (TPH) in oily sludge reaches more than 70.5% within 2 weeks; The aromatics in the oil have a significant degradation effect, and the degradation rate of the aromatics in the total petroleum hydrocarbons in the oily sludge reaches 69.2% within 2 weeks.

Description

technical field [0001] The invention relates to the technical field of biochemical treatment of oily sludge; in particular, to an oily sludge degrading strain Proteus mirabilis SB and its application. Background technique [0002] The biological treatment of oily sludge mainly uses the metabolism of microorganisms to degrade petroleum hydrocarbons and finally convert them into CO 2 and H 2 O. The more representative ones are the composting method and the bio-enhancing method. The former is to fully stir the oily sludge and the consumable substances required for biological treatment, and then stack them in rows, and use the aeration truck to regularly stir them to make them fully react. The latter is a microbial inoculum that can efficiently decompose petroleum hydrocarbons into oily sludge. These microorganisms use petroleum hydrocarbons as a carbon source for metabolism, and use the metabolic activities of microorganisms to assimilate and degrade petroleum hydrocarbons an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/02C12R1/01C02F101/32
CPCC02F3/34C02F3/02C02F2101/32Y02W10/10
Inventor 柯从玉秦芳玲张群正孙妩娟张洵立王嗣昌
Owner 陕西大胜石油工程技术服务有限公司
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