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Application of chitosan oligosaccharide selenium in relieving pig intestinal barrier injury caused by F-2 toxin

A chitosan oligosaccharide and barrier damage technology, applied in the chitosan oligosaccharide, cell engineering, and the field of alleviating F-2 toxin-induced intestinal barrier damage in pigs, can solve problems such as chitosan oligosaccharide and selenium that have not been seen

Inactive Publication Date: 2021-07-27
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no patent publications or reports on the application of chitosan oligosaccharide selenium in resisting F-2 toxin damage to the intestinal barrier of pigs at home and abroad

Method used

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  • Application of chitosan oligosaccharide selenium in relieving pig intestinal barrier injury caused by F-2 toxin
  • Application of chitosan oligosaccharide selenium in relieving pig intestinal barrier injury caused by F-2 toxin
  • Application of chitosan oligosaccharide selenium in relieving pig intestinal barrier injury caused by F-2 toxin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] Chitosan oligosaccharide selenium alleviates the method that F-2 toxin causes pig jejunum intestinal barrier damage, and the steps are as follows:

[0091] For porcine jejunum epithelial cells, chitosan-oligosaccharide selenium (final concentration in the culture medium) with a selenium concentration of 0.8 and 2.0 μmol / L was added respectively. After culturing for 6 hours, F-2 toxin was added to them respectively to make them The concentration was 20 µg / mL, and the culture ended after 24 hours of continuous culture. The effect of inhibiting the decrease of the tight junction protein ZO-1 protein level of porcine jejunum epithelial cells caused by F-2 toxin and weakening the effect of F-2 toxin on the barrier damage of porcine jejunum is achieved.

[0092] The verification method of chitosan oligosaccharide selenium alleviating F-2 toxin-induced intestinal barrier damage in porcine jejunum, the steps are as follows:

[0093] 1.1 Porcine jejunum epithelial cell culture ...

Embodiment 2

[0123] Chitosan oligosaccharide selenium alleviates the method that F-2 toxin causes pig ileum intestinal barrier damage, and the steps are as follows:

[0124] For porcine ileal epithelial cells, chitosan-oligosaccharide selenium (final concentration in the culture medium) with a selenium concentration of 1.5 and 3.0 μmol / L was added respectively, and after 12 hours of culture, F-2 toxin was added to them respectively, so that Its concentration was 40 μg / mL, and the culture ended after 12 hours of continuous culture. The effect of inhibiting the decrease of the tight junction protein ZO-1 protein level of porcine ileal epithelial cells caused by F-2 toxin and weakening the effect of F-2 toxin on the intestinal barrier damage of porcine ileum is achieved.

[0125] The verification method for chitosan oligosaccharide selenium to alleviate the intestinal barrier damage of pig ileum caused by F-2 toxin, the steps are as follows:

[0126] 2.1 Porcine ileal epithelial cell culture...

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Abstract

The invention discloses an application of chitosan oligosaccharide selenium in relieving pig intestinal barrier injury caused by F-2 toxin. The method for relieving the F-2 toxin-caused pig intestinal barrier injury means that the oligochitosan selenium inhibits the F-2 toxin-caused pig intestinal epithelial cell tight junction protein ZO-1 protein level reduction. Experimental results show that 0.8 to 3.0 [mu] mol / L of chitosan oligosaccharide selenium is used for porcine small intestine epithelial cells, and when the concentration of F-2 toxin is 20 to 40 [mu] g / mL, the decrease of the protein level of tight junction protein ZO-1 of the porcine small intestine epithelial cells caused by the F-2 toxin can be inhibited, so that the effect of weakening the damage of the F-2 toxin to the intestinal barrier of the porcine small intestine is achieved, and a technical support is provided for the application of the chitosan oligosaccharide selenium in the aspects of preventing and treating F-2 toxin poisoning of pigs, especially relieving intestinal barrier injury and maintaining normal functions of intestinal tracts.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to cell engineering technology, in particular to an application and method of chitosan oligosaccharide selenium for alleviating F-2 toxin-induced intestinal barrier damage in pigs. Background technique [0002] Zearalenone (ZEN), also known as F-2 toxin, its toxic effects mainly include: reproductive and developmental toxicity, liver toxicity, immunotoxicity, and genotoxicity. Corresponding toxic effects are also produced; the intestinal tract is the initial barrier of mycotoxins, and the barrier protection of the intestinal mucosa is essential for intestinal health. [0003] As an essential trace element for the body, selenium has various biological functions such as anti-oxidation, improving the body's immunity and disease resistance, regulating the body's metabolism, affecting the reproductive function of animals and humans, anti-tumor, delaying aging, and antagonizing toxic elements. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/04A61K31/722A61P1/00A61P39/02G01N33/68
CPCA61K31/722A61K33/04A61P1/00A61P39/02G01N33/6893G01N2800/065A61K2300/00
Inventor 秦顺义李留安傅春妮于巧玲赵瑞利罗安智张建斌李元辉金天明刘田生
Owner TIANJIN AGRICULTURE COLLEGE
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