Antifibrin-2 antibody sequence, tetrapeptide chain molecule and immunoglobulin molecule

A fibrin and sequence technology, applied in the direction of antiviral immunoglobulin, immunoglobulin, peptide, etc.

Active Publication Date: 2021-07-27
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The difficulty in solving the above problems and defects is: in order to obtain an antibody library of a certain scale, the first thing to do is to obtain a complete set of antibody genes from the body with the help of PCR technology. , heavy chain variable region sequence for multiple pairs of primers verification

Method used

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  • Antifibrin-2 antibody sequence, tetrapeptide chain molecule and immunoglobulin molecule
  • Antifibrin-2 antibody sequence, tetrapeptide chain molecule and immunoglobulin molecule
  • Antifibrin-2 antibody sequence, tetrapeptide chain molecule and immunoglobulin molecule

Examples

Experimental program
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Effect test

Embodiment 1

[0063] Example 1: Construction of chicken-derived antiserum type 4 avian adenovirus fibrin-2 single-chain antibody library

[0064] 1. Isolation of Chicken Peripheral Blood Lymphocytes

[0065] According to the International Animal Immunization Manual, 5 experimental chickens were immunized with serotype 4 avian adenovirus inactivated vaccine. Each immunization interval was 2 weeks, and a total of 3 immunizations were performed. Two weeks after the third immunization, the peripheral blood of the chickens was collected. Take 5-10mL of fresh blood and mix evenly with whole blood and tissue diluent at a ratio of 1:1-1:2. Add cell separation solution to a 15mL centrifuge tube, and gently add an equal volume of diluted anticoagulant blood along the tube wall. The horizontal centrifuge is centrifuged at a speed of 400g-800g and a time of 15min-25min. After centrifugation, the liquid in the centrifuge tube should be divided into four layers, from top to bottom: the first layer: pl...

Embodiment 2

[0072] Example 2: Screening of chicken-derived antiserum type 4 avian adenovirus fibrin-2 single-chain antibody library

[0073] 1. Preparation of Phage Antibody

[0074] Add 1 mL of phage primary antibody library to 3 mL of XLI-Blue bacterial solution with OD600=0.5, place at 37°C for 45 min, add 6 mL of LB liquid medium containing Amp+ (100 μg / mL), and add glucose (1 mol / mL) at a ratio of 1:1000 L) Continue to cultivate, when the OD600 of the bacterial solution is 0.5, add the helper phage M13K07, place it in a 37°C incubator for 30min, and then incubate at 220rpm / min at 37°C for 1h, centrifuge the bacterial solution at 3500rpm for 10min, discard the supernatant, and use an equal volume LB liquid medium containing Amp+ (100 μg / mL) and Kana (50 μg / mL) was added with IPTG (1 mol / L) at a ratio of 1:1000, 220 rpm / min, 37°C, and shaken for 6 hours. Centrifuge the culture medium at 12000rpm for 10-20min, and collect the supernatant. Add PEG8000 at a ratio of 1:4-1:5, invert and ...

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Abstract

The invention belongs to the technical field of virus protein antibodies, and discloses an antifibrin-2 antibody sequence, a tetrapeptide chain molecule and an immunoglobulin molecule, the virus protein antibody sequence is characterized in that the amino acid sequence of a heavy chain variable region is SEQ ID NO: 1, and the nucleotide sequence is SEQ ID NO: 3; the amino acid sequence of a light chain variable region is SEQ ID NO: 2, and the nucleotide sequence is SEQ ID NO: 4. The amino acid sequences and the nucleotide sequences of the variable regions of the heavy chain and the light chain of the chicken-derived antiserum type 4 fowl adenovirus fibrin-2 antibody provided by the invention provide support for constructing a gene engineering antibody of the chicken-derived antiserum type 4 fowl adenovirus fibrin-2 with high affinity and low immunogenicity, and are of great significance in promoting the diagnosis of serum type 4 avian adenovirus infection and the development of chicken-derived antibody drugs.

Description

technical field [0001] The invention belongs to the technical field of virus antibodies, and in particular relates to an antiserum type 4 avian adenovirus fibrin-2 antibody sequence, tetrapeptide chain molecules, immunoglobulin molecules and applications. Background technique [0002] Serum type 4 avian adenovirus belongs to the avian adenoviridae family and is a non-enveloped double-stranded linear DNA virus. According to different antigenicity, avian adenovirus can be divided into avian adenovirus-I group (Fowl adenovirus, FAdVs), II group and III group. According to molecular characteristics and restriction endonuclease analysis, FAdVs can be divided into 5 species, A-E, and 12 serotypes (namely 1-8a and 8b-11) according to the results of serum cross-neutralization test. FAdVs infected chickens mainly caused gizzard erosion, inclusion body hepatitis and pericardial effusion-hepatitis syndrome, etc., and also caused slow growth and prolonged immunosuppression leading to i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/08C12N15/13C12N15/70G01N33/569A61P31/20
CPCC07K16/081C07K16/005C12N15/70G01N33/56983A61P31/20C07K2317/56C07K2317/23C07K2317/622G01N2333/075Y02A50/30
Inventor 王建琳尹燕博徐守振曲雪婷高倩文
Owner QINGDAO AGRI UNIV
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