Pretreatment method for detecting geosmin substances in aquatic products

A technology for aquatic products and odor, which is applied in the field of pretreatment for the detection of earthy odor substances in aquatic products. It can solve the problems of long process time, lost target objects, and the recovery rate of earthy odor substances is less than 50%. The effect of improving the efficiency, enhancing the applicability, saving the pre-processing time and the cost of consumables

Active Publication Date: 2021-07-27
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The limitations of the above methods are in the pre-processing. First, the steps are cumbersome, time-consuming and expensive.
The distillation method of microwave extraction first, and then the adsorption extraction method of solid-phase microextraction are extremely time-consuming and laborious. The process takes a long time, and it cannot be carried out in batches, especially the service life of the solid-phase fibe

Method used

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  • Pretreatment method for detecting geosmin substances in aquatic products
  • Pretreatment method for detecting geosmin substances in aquatic products
  • Pretreatment method for detecting geosmin substances in aquatic products

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Embodiment 1

[0026] (1) Take about 5 g of edible tissues of aquatic products and cut them into pieces. Place in a 50 mL stoppered centrifuge tube. Then add 0.5 g papain, 2 mL double distilled water. Vortex for 1 min to mix. Place the centrifuge tube in a 37°C water bath and let it stand for 30 minutes. This step utilizes the effect of enzymatic hydrolysis to change the massive structure of the aquatic product into an emulsion, which facilitates the separation of the target analyte from the tissue and the next step of extraction.

[0027] (2) Add 15 mL of n-hexane, vortex for 1 minute, and mix well. As for 15 minutes in the ultrasonic bath. Centrifuge at 10619 g for 8 min. This step is repeated once. Combine the two supernatants and transfer to a new 50 mL stoppered centrifuge tube.

[0028] (3) Add the purification extraction package. The material and dosage formula of the purification extraction package are as follows: 1.5 g of propylethylenediamine (40-70 μm in particle size), 1....

Embodiment 2

[0045] A pretreatment method for detecting earthy odor substances in aquatic products, the steps are as follows:

[0046] Step 1. Take the edible tissue of the aquatic product, cut it into pieces, put it into a centrifuge tube with a stopper, then add papain, double distilled water, vortex, mix well, put the centrifuge tube in a water bath, let it stand, and the aquatic product is edible The ratio of tissue, papain, and double distilled water is 10 g: 1 g: 4 mL, vortex for 2 minutes, mix well, place the centrifuge tube in a 37°C water bath, and let it stand for 50 minutes;

[0047] Step 2. Then add n-hexane to the stoppered centrifuge tube treated in step 1, vortex for 1 minute, and mix well. The ratio of n-hexane to edible tissue of aquatic products is 20 mL: 7.5 g, and place in an ultrasonic bath for 20 Minutes, then centrifuge at 10619 g for 8 minutes, take the supernatant, repeat this step once, and transfer the supernatant twice to a new stoppered centrifuge tube;

[004...

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Abstract

A pretreatment method for detecting geosmin substances in aquatic products comprises the following steps: taking edible tissues of a water product, cutting the edible tissues into pieces, putting the pieces into a centrifuge tube with a plug, then adding papain and double distilled water, carrying out vortex treatment, uniformly mixing, and standing in a water bath; then adding n-hexane, whirling, uniformly mixing, placing in an ultrasonic bath, centrifuging, taking the supernatant, repeating the steps in sequence, merging the two supernatants, and transferring into a new centrifuge tube with a plug; adding a purification extraction bag into the centrifugal tube with the plug; carrying out vortex and centrifugation on the centrifugal tube, taking supernate, transferring the supernate to a new centrifugal tube, and sealing a cover for later use; using a solid-phase extraction column, using normal hexane for leaching, usingethyl acetate for elution, and after eluent passes through an organic phase filter membrane, performing GC-MS automatic sample injection and detection. According to the method, the pretreatment time and the use cost of consumables are greatly saved, the detection recovery rate of the geosmin substances in the aquatic products can be increased to about 80%, the treatment method is simple and can be operated in batches, and the applicability of the method is also enhanced in the aspect of quality control.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular to a pretreatment method for detecting earthy odor substances in aquatic products. Background technique [0002] The aquatic products provided by aquaculture have greatly met the demand for protein of the Chinese people and are the unshakable foundation for maintaining national food security. However, some odorous substances remaining in aquatic products greatly affect the taste of eating. These odor substances are mainly earthy odor substances, mainly including geosmin, 2-methylisoborneol, 2-isobutyl-methoxypyrazine, 2-isopropyl-methoxypyrazine and 2 , 3,6-trichloroanisole, among them, geosmin and 2-methylisoborneol contribute the most to the earthy odor of aquatic products, and are currently the most relevant researches. [0003] In the existing research reports in my country, geosmin and 2-methylisoborneol have been detected in many freshwater cultured species such as big...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/24
CPCG01N30/02G01N30/06G01N30/14G01N30/24G01N2030/062G01N2030/146Y02A40/81
Inventor 宋超邹剑敏陈家长桂源孟顺龙陈曦
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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