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An organic-inorganic hybrid material for single-molecule detection and its preparation method

A single-molecule detection and hybrid material technology, applied in the field of nanomaterials, can solve the problems of weak fluorescence intensity and limitation of gold nanoclusters, and achieve the effect of fast detection speed and high sensitivity

Active Publication Date: 2022-05-13
深圳市光与生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, the weak fluorescence intensity of gold nanoclusters is limited in their use as fluorescent markers for single-molecule immunoassays.

Method used

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  • An organic-inorganic hybrid material for single-molecule detection and its preparation method

Examples

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Embodiment 1

[0031] Example 1: Preparation of modified polyphenylene sulfide.

[0032] Take a piece of dried polyphenylene sulfide with a number-average molecular weight of 20,000 g / mol, weigh 1 g, and add it to a 50-mL branch-mouthed flask. 3 The oleum was mixed well, heated in a water bath at 100 °C for 1 h, the crude product was ultrafiltered with collodion, ethanol and ether were added, and after standing for half an hour, centrifuged, the supernatant was removed, ethanol and ether were added, and the centrifugation was repeated. 3 times, and after vacuum drying, water-soluble sulfonated polyphenylene sulfide is obtained.

[0033] Weigh 30ml of concentrated nitric acid into 70ml of deionized water to form a concentrated nitric acid solution with a volume fraction of 30%, then add 0.5g of the water-soluble sulfonated polyphenylene sulfide prepared above, and treat in a water bath at 80°C for 2 hours. The modified polyphenylene sulfide product was washed with deionized water and ethanol...

Embodiment 2

[0034] Example 2: Preparation of modified polyphenylene sulfide-modified gold nanoparticles.

[0035]The toluene solution of cetyltrimethylammonium bromide (6mL, 44.4mM), the aqueous solution of chloroauric acid (20mL, 0.6mol / L) and the cosurfactant n-butanol (0.5mL) were added to 50mL of In the conical flask, after all the chloroauric acid was transferred to the organic layer, add modified polyphenylene sulfide (500mg, 7mM); after the modified polyphenylene sulfide was dissolved, slowly add the newly prepared sodium citrate solution ( 2mL, 0.05mol / L), stirred at room temperature for 6h, stopped stirring after the reaction, poured the upper layer solution into a single-necked flask for rotary evaporation, concentrated the toluene solvent to 2mL, added 400mL of anhydrous ethanol, stored at -4°C for 8h and centrifuged , separate the ethanol solution, repeat the centrifugation twice, put the obtained modified polyphenylene sulfide-modified gold nanoparticles in a vacuum drying ov...

Embodiment 3

[0036] Example 3: Preparation of organic-inorganic hybrid materials for single molecule detection.

[0037] Under a constant temperature water bath of 40°C, 220 mL of absolute ethanol, 5 mL of deionized water, and 14 mL of ammonia water were added to a 500 mL one-neck flask; under constant stirring, 11.2 mL of tetraethyl orthosilicate was added, and after the reaction for 12 h, the reaction solution was centrifuged and anhydrous Ethanol washing, deionized water dispersion, freeze-drying to obtain nano-silica.

[0038] Weigh 3.5 g of the above-obtained nano-silicon dioxide into a 250-mL two-necked flask, add 175 mL of anhydrous ethanol, and uniformly disperse by ultrasonic to obtain a nano-silica suspension. Add 157mL of absolute ethanol and 17mL of deionized water to another 250mL two-necked flask, add 105μL of glacial acetic acid to adjust the pH to 5, add 7g of γ-aminopropyltrimethoxysilane dropwise, stir to dissolve it, and dissolve the nano-dioxide. The silicon suspension...

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Abstract

The invention discloses an organic-inorganic hybrid material for single-molecule detection and a preparation method thereof. The organic-inorganic hybrid material for single-molecule detection is coated with silicon dioxide and modified polyphenylene sulfide. Assembled fluorescent gold nanoclusters. The present invention uses modified polyphenylene sulfide as a template to synthesize gold nanoclusters, because the modified polyphenylene sulfide contains a large number of hydroxyl groups, carboxyl groups and sulfonate groups, and at the same time, it also contains complexes that can coordinate with transition metal gold. By forming a thioether group, the gold nanoclusters can be stably and uniformly distributed in water. In addition, when silica is grafted on the surface of modified polyphenylene sulfide-modified gold nanoparticles, the transition metal gold and the benzene ring and silica on the polymer molecular chain of modified polyphenylene sulfide resonate, and the fluorescence can be expressed as It is emitted in the form of stronger light intensity and faster decay speed, and an organic-inorganic hybrid material with high sensitivity and fast detection speed is obtained.

Description

technical field [0001] The invention belongs to the field of nanomaterials and biology, and in particular relates to an organic-inorganic hybrid material for single molecule detection and a preparation method thereof. Background technique [0002] Single-molecule detection technology has the advantages of high detection sensitivity, simple quantitative method, and low sample demand, and is widely used in biological detection, environmental safety, drug tracer, biotechnology, single-particle nanocatalysis, sensors and other fields; in complex systems , Interfering substances are prone to non-specific interactions with molecular probes. When the concentration of the detection substances in the detection system is low, it is difficult for conventional detection methods to achieve higher sensitivity and lower detection limits, so as to accurately detect and detect substance. Single-molecule detection technology is different from conventional detection technology. It can detect ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G83/00G01N21/64
CPCC08G83/001G01N21/64G01N21/6428G01N2021/6417G01N2021/6439
Inventor 何皓
Owner 深圳市光与生物科技有限公司
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