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Tunneling magnetoresistive biosensor and preparation method and application thereof

A biosensor and tunneling magneto-resistance technology, applied in magnetic sensor arrays, instruments, scientific instruments, etc., can solve the problems of low sensitivity of colloidal gold method and easy occurrence of missed detection, so as to improve detection sensitivity and detection rate , Improve the effect of signal-to-noise ratio

Pending Publication Date: 2021-07-30
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The existing novel coronavirus-specific antibody detection methods are colloidal gold method and magnetic particle chemiluminescence method. The sensitivity of colloidal gold method is not high and it is prone to missed detection.
The magnetic particle chemiluminescence method requires special chemiluminescence detection equipment, which is not suitable for on-site detection in communities, airport courts, etc.

Method used

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  • Tunneling magnetoresistive biosensor and preparation method and application thereof
  • Tunneling magnetoresistive biosensor and preparation method and application thereof

Examples

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Embodiment 1

[0049] (1) The tunneling magnetoresistive sensor chip is composed of a CoFe / CoFeB / MgO / CoFeB multilayer film on a silicon wafer, which is in a zigzag shape. The multilayer film structure is deposited by magnetron sputtering, and the CoFe and MgO layers are passed through radio frequency pattern deposition.

[0050] (2) Plating a protective layer of Al on the surface of the tunneling magnetoresistive sensor by atomic layer deposition 2 o 3 , Al 2 o 3 The thickness is 20nm.

[0051] (3) In the protective layer Al 2 o 3 A protective layer and a biological binding layer SiO are plated on the surface by plasma chemical vapor deposition 2 , SiO 2 The thickness is 20nm.

[0052] (4) Wash the tunneling magnetoresistive sensor three times with acetone, methanol, and isopropanol for 10 minutes, dry with nitrogen, and treat with oxygen plasma for 30 minutes. The treated sensor was treated in 2% (3-aminopropyl) triethoxysilane (absolute ethanol) solution for 30 minutes, dried in a...

Embodiment 2

[0056] (1) The tunneling magnetoresistive sensor chip is composed of a CoFe / CoFeB / MgO / CoFeB multilayer film on a silicon wafer, which is in a zigzag shape. The multilayer film structure is deposited by magnetron sputtering, and the CoFe and MgO layers are passed through radio frequency pattern deposition.

[0057] (2) Plating a protective layer of Al on the surface of the tunneling magnetoresistive sensor by atomic layer deposition 2 o 3 , Al 2 o 3 The thickness is 18nm.

[0058] (3) In the protective layer Al 2 o 3 A protective layer and a biological binding layer SiO are plated on the surface by plasma chemical vapor deposition 2 , SiO 2 The thickness is 20nm.

[0059] (4) Wash the tunneling magnetoresistive sensor three times with acetone, methanol, and isopropanol for 10 minutes, dry with nitrogen, and treat with oxygen plasma for 30 minutes. The processed sensor was treated in 5% (3-aminopropyl) triethoxysilane (absolute ethanol) solution for 1 hour, dried in an ...

Embodiment 3

[0063] (1) The tunneling magnetoresistive sensor chip is composed of a CoFe / CoFeB / MgO / CoFeB multilayer film on a silicon wafer, which is in a zigzag shape. The multilayer film structure is deposited by magnetron sputtering, and the CoFe and MgO layers are passed through radio frequency pattern deposition.

[0064] (2) Plating a protective layer of Al on the surface of the tunneling magnetoresistive sensor by atomic layer deposition 2 o 3 , Al 2 o 3 The thickness is 20nm.

[0065] (3) In the protective layer Al 2 o 3 A protective layer and a biological binding layer SiO are plated on the surface by plasma chemical vapor deposition 2 , SiO 2 The thickness is 50nm.

[0066] (4) Wash the tunneling magnetoresistive sensor three times with acetone, methanol, and isopropanol for 10 minutes, dry with nitrogen, and treat with oxygen plasma for 30 minutes. The treated sensor was treated in 2% (3-aminopropyl) triethoxysilane (absolute ethanol) solution for 1 hour, dried in an ov...

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Abstract

The invention discloses a tunneling magnetoresistive biosensor and a preparation method and application thereof. The biosensor comprises a tunneling magnetoresistance multilayer film structure, a reaction area of the tunneling magnetoresistance biosensor, a biological sensitive film in the reaction area and magnetic beads for generating signals. The reaction area of the tunneling magnetoresistive biosensor is divided into a novel coronavirus IgM antibody detection area, a negative control area and a novel coronavirus IgG antibody detection area, the novel coronavirus IgM antibody detection area fixes an anti-human IgM antibody, the negative control area modifies bovine serum albumin, and the novel coronavirus IgG antibody detection area fixes an anti-human IgG antibody. The tunneling magnetoresistive biosensor is more accurate in result, high in sensitivity, simple and convenient to operate, free of operation by professionals, low in detection cost and suitable for field detection of communities, airports, families and the like, and an effective means is provided for rapid screening of novel coronaviruses.

Description

technical field [0001] The invention belongs to the technical field of biological detection sensors, in particular to a tunneling magnetoresistive biosensor, a preparation method and application thereof. Background technique [0002] At present, the main detection method for the new coronavirus is fluorescent PCR. However, the positive rate of nucleic acid detection of nasopharyngeal swabs is only 30%-50%, and there are a large number of false negative results. The detection principle of fluorescent PCR is to amplify the target sequence in the sample. After amplification, the amount of nucleic acid is sufficient for detection by fluorescence. Therefore, the positive rate of detection is closely related to the amount of nucleic acid. Due to the different virus content in patients with different disease courses and different conditions; the new coronavirus with single-stranded RNA is easily degraded by RNase; the virus genome may mutate, and the sensitivity and specificity of ...

Claims

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Application Information

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IPC IPC(8): G01N27/74G01R33/12G01R33/00G01R33/09G01N33/543G01N33/569
CPCG01N27/745G01R33/1269G01R33/1253G01R33/0094G01R33/098G01R33/091G01N33/56983G01N33/54346G01N33/54326
Inventor 张晓慧刘明张翠玲
Owner XI AN JIAOTONG UNIV
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