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A strain of Pseudoalteromonas piscicida and its application in aquaculture

A technology of pseudoalteromonas and aquaculture, applied in the field of microorganisms, can solve problems such as difficult to achieve antagonistic pathogenic bacteria and immune function, promote intestinal function, difficult and efficient application of bacterial disease technology, and few application technology reports, etc., to achieve Improve intestinal function, improve length, increase the length of the effect

Active Publication Date: 2022-05-03
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fermenting fish and shrimp feed with Lactobacillus can increase the utilization rate of feed, improve the intestinal flora structure of farmed animals, and reduce the colonization of pathogenic bacteria; There are many, and the existing strains generally have a single function, and it is difficult to achieve the synergistic effect of antagonizing pathogenic bacteria, immune function, and promoting intestinal function, making it difficult to efficiently apply the existing microbial prevention and control of bacterial diseases in aquaculture

Method used

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  • A strain of Pseudoalteromonas piscicida and its application in aquaculture
  • A strain of Pseudoalteromonas piscicida and its application in aquaculture
  • A strain of Pseudoalteromonas piscicida and its application in aquaculture

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Study on the Effect of Pseudoalteromonas Fischeri 2013082515 in Shrimp Culture

[0050] 1. Material method

[0051] 1.1 Preparation of Pseudoalteromonas Fischeri 2013082515 Preparation

[0052] A strain of Pseudoalteromonas fischerii 2013082515 stored at -80℃ is crossed on a solid medium of 2216E, and a single colony is obtained by culturing at 28℃ for 24h. A single colony is inoculated into the seed culture solution of Pseudoalteromonas fischerii, and then the seed solution is inoculated into the fermentation medium of Pseudoalteromonas fischerii for 24h, and the temperature is controlled at 28℃ When the OD600 value of the fermentation broth reaches 1.5 ~ 1.7, the fermentation is stopped to obtain the fermentation broth, and the strain concentration of the fermentation broth is determined by plate counting.

[0053] The 16S rDNA base sequence of Alternaria piscicida 2013082515 strain is shown in seq id no: 1.

[0054] SEQ ID NO：1：

[0055]AGCAACCCACTCCCATGGTGTGACGGGCGGTGTG...

Embodiment 2

[0090] Study on the Effect of Alternaria Fischeri 2013082515 on Paralichthys olivaceus Culture

[0091] 1. Material method

[0092] 1.1 Preparation of Pseudoalteromonas Fischeri 2013082515 Preparation

[0093] A strain of Pseudoalteromonas fischerii 2013082515 stored at -80℃ is crossed on a solid medium of 2216E, and a single colony is obtained by culturing at 28℃ for 24h. A single colony is inoculated into the seed culture solution of Pseudoalteromonas fischerii, and then the seed solution is inoculated into the fermentation medium of Pseudoalteromonas fischerii for 24h, and the temperature is controlled at 28℃ When the OD600 value of the fermentation broth reaches 1.5 ~ 1.7, the fermentation is stopped to obtain the fermentation broth, and the strain concentration of the fermentation broth is determined by plate counting.

[0094] 1.2 experimental flounder

[0095] The experimental PARALICHTHYS olivaceus, 12 ~ 14 cm long, was purchased from a PARALICHTHYS olivaceus breeding ente...

Embodiment 3

[0131] Study on the Effect of Pseudoalteromonas Fischeri 2013082515 on the Breeding of Bay Scallop

[0132] 1. Material method

[0133] 1.1 Preparation of Pseudoalteromonas Fischeri 2013082515 Preparation

[0134] A strain of Pseudoalteromonas fischerii 2013082515 stored at -80℃ is crossed on a solid medium of 2216E, and a single colony is obtained by culturing at 28℃ for 24h. A single colony is inoculated into the seed culture solution of Pseudoalteromonas fischerii, and then the seed solution is inoculated into the fermentation medium of Pseudoalteromonas fischerii for 24h, and the temperature is controlled at 28℃ When the OD600 value of the fermentation broth reaches 1.5 ~ 1.7, the fermentation is stopped to obtain the fermentation broth, and the strain concentration of the fermentation broth is determined by plate counting.

[0135] 1.2 experimental bay scallop larvae

[0136] The larvae of bay scallop were used as D-type larvae.

[0137] 1.3 Dosage of Pseudoalteromonas Fische...

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Abstract

The invention belongs to the technical field of microbes, and in particular relates to the application of a strain of Pseudoalteromonas piscicida in fish and shrimp cultivation. The strain was deposited at the China Center for Type Culture Collection in Wuhan on November 12, 2020, with the deposit number: CCTCC NO:M 2020731. The strain has broad-spectrum anti-vibrio activity, and has strong antagonistic activity against Aeromonas salmonicida. It can also enhance the expression of fish lysozyme, tumor necrosis factor and cell interleukin gene after oral administration, and can significantly Improve the immune level of the body fluid of aquaculture animals. In addition, the strain can also significantly increase the length of the intestinal microvilli of aquaculture animals, which tends to increase the muscle thickness and the length of the villi, which is conducive to improving the intestinal function of aquaculture animals. , realize the synergistic effect of antagonizing pathogenic bacteria, immunity, and promoting intestinal function, and more effectively prevent and control bacterial diseases in aquaculture.

Description

Technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to the application of a pseudoalteromonas sp. killing fish in fish and shrimp culture. technical background [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgement or any form of implication that this information constitutes the prior art known to those of ordinary skill in the art.. [0003] There are many kinds of microorganisms in the natural environment, some of which can interact with each other, including symbiosis, symbiosis, parasitism, antagonism and predation. In order to control the proliferation of pathogenic microorganisms in the environment and organisms, the antagonistic characteristics of microorganisms can be used to control diseases. At present, the research and development of probiotic products to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/741A61P31/04A61P37/04A23K50/80A23K10/18A01K61/13C12R1/01
CPCA61K35/741A61P31/04A61P37/04A23K50/80A23K10/18A01K61/13Y02A40/81
Inventor 王秀华王枫林杨冰张庆利
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI