Check patentability & draft patents in minutes with Patsnap Eureka AI!

Silicon dioxide nano particles with bent spikes and preparation method thereof

A nanoparticle and silicon dioxide technology, applied in the field of biomedical chemistry, can solve the problems of slow biodegradation, single function, and uncontrollable number and shape of spikes, and achieve high cell survival rate, high transfection efficiency, and improved reliability. Applied Effects

Active Publication Date: 2021-08-17
上海千溯生物科技有限公司
View PDF7 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although the existing research can prepare some anti-virus structure silica nanoparticle materials, the size of the prepared nanomaterials is generally too large, it is difficult to control within 100nm or even 80nm, and the smaller size materials are easier to penetrate biofilm / crossover biological barrier
At the same time, in the existing research, there are often only two options: growing spikes in situ or not growing spikes, and the number and shape of spikes cannot be controlled, thus limiting the application scenarios of nanomaterials
In addition, the known silica nanomaterials biodegrade slowly and have a single function, which is difficult to meet the increasingly complex biological application scenarios.
[0004] In addition, the spikes of silica nanoparticles prepared in the prior art are all long and straight, and cannot be prepared into other shapes

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Silicon dioxide nano particles with bent spikes and preparation method thereof
  • Silicon dioxide nano particles with bent spikes and preparation method thereof
  • Silicon dioxide nano particles with bent spikes and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1: Preparation of silica nanoparticles with different numbers of nanospikes

[0051] In 20 mL of water, CTAB was added, the stirring speed was 500 rpm, and the temperature was 60 °C, stirring until CTAB was fully dissolved, then 0.01 g of NaOH was added, and stirring was continued for 30 minutes, and then a mixed solution of cyclohexane and TEOS (4.68 g of cyclohexane was added) was added. alkane and 1.4 g TEOS), and continued to react at 60° C. with stirring for 48 hours to obtain a silica nanomaterial with a virus-like structure. In this specific reaction process, when the amount of CTAB is 0.15g, 0.2g, and 0.3g respectively (when the CTAB concentration is 0.75wt%, 1.0wt%, and 1.5wt%, respectively), the obtained virus-like structure silica nanoparticles The number of surface nanopillars is: 10 to 15 (such as figure 1 shown), 30 to 35 (such as figure 2 shown), 50 to 60 (such as image 3 shown).

Embodiment 2

[0052] Example 2: Preparation of silica nanoparticles with different core diameters

[0053] 1. Obtain small size (30-80nm) silica nanoparticles: by mass ratio, CTAB:NaOH:H 2 O=(0.1~2): 0.05:100, CTAB, NaOH mixed aqueous solution was prepared at 60 degreeC. Then add TEOS and cyclohexane, according to the mass ratio, CTAB:NaOH:TEOS:cyclohexane:H 2 O=(0.1~2): 0.05:7:23:100, at 60°C, with rapid stirring (400~1500rpm), a stable microemulsion system is formed. The stirring reaction was continued for 48 hours to obtain a silica nanomaterial with a virus-like structure.

[0054] The size of the virus-like silica nanoparticles can be precisely tuned by adjusting the stirring speed, while simultaneously adjusting the initial concentration of CTAB. The control parameters, corresponding kernel size and corresponding diagram are shown in Table 1.

[0055] Table 1 Silica nanoparticles size control comparison table

[0056]

[0057]

[0058] We found that if only the stirring spe...

Embodiment 3

[0066] Example 3: Preparation of silica nanoparticles with special shaped spikes

[0067] 1. Control the shape of the spike by changing the reaction temperature.

[0068] In 20mL of water, add 0.4g CTAB, stir at a speed of 600rpm and a temperature of 70°C, stir until CTAB is fully dissolved, then add 0.01g of NaOH, continue stirring for 30 minutes, and then add a mixed solution of cyclohexane and TEOS (4.68g cyclohexane and 1.4g TEOS), continue to react at 70° C. with stirring for 48 hours, and obtain a silica nanomaterial with a virus-like structure with obvious curved spikes. Electron microscope scan as Figure 14 shown.

[0069] 2. Control the shape of the spike by changing the catalyst.

[0070] In 20mL of water, add 0.4g CTAB, stir at a speed of 600rpm and a temperature of 60°C, stir until CTAB is fully dissolved, then add 0.056g of tetrapropylammonium hydroxide, continue stirring for 30 minutes, and then add a mixture of cyclohexane and TEOS. The mixed solution (4.68...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of biomedical chemistry, and particularly relates to virus-like silicon dioxide nano particles with bent spikes and a preparation method thereof. According to the specific technical scheme, the silicon dioxide nano particle comprises an inner core and spikes, the diameter of the inner core is 5-100 nm, and a plurality of nano spikes controllable in number and shape grow on the surface of the inner core in situ. The invention provides the small-size virus-like silicon dioxide nano particle capable of accurately controlling the kernel diameter, the spike number and the spike shape for the first time; further synthesizing into functional virus-shaped silicon dioxide nano particles capable of being quickly degraded is realized; and a transfection kit is further prepared from the nano particles, so that the transfection efficiency is high, and the cell survival rate is high.

Description

technical field [0001] The invention belongs to the field of biomedical chemistry, and particularly relates to silica nanoparticles with curved spines and a preparation method thereof. Background technique [0002] Compared with traditional drugs, nanotechnology-based drug delivery, targeting of diagnosis and treatment systems, and bioavailability have obvious advantages. In a variety of complex applications, we often hope that nanocarriers can quickly adhere to biofilms like viruses, and are abundantly enriched at the site of lesions. At present, the research on virus-like nanoparticles is relatively limited, and if the surface chemistry is modified, it may lead to increased cytotoxicity; if a rough surface structure is constructed on the surface of such materials, the known construction process is very complicated, and The construction process is poorly controllable. Therefore, in-situ growth of some nanostructures on the surface of materials to form antivirus structures...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C01B33/18B82Y30/00B82Y40/00
CPCC01B33/18B82Y30/00B82Y40/00C01P2004/03C01P2004/64
Inventor 徐天兆
Owner 上海千溯生物科技有限公司
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com