Controllable crosslinking and degrading material and application thereof
A technology of synthetic materials and molecules, which is applied in the fields of application, medical science, and pharmaceutical formulations, can solve the problems of no interaction between tissues, low strength of colloids, and inconvenient use, and achieve strong hemostasis ability, overcome insufficient water absorption, Meet the effect of pressing to stop bleeding
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[0041] Preparation of materials:
[0042] 1. The preparation method of four-arm oxyethylene polyoxypropylene polyoxyethylene succinimide ester
[0043] Four-armed oxyethylene polyoxypropylene polyoxyethylene (0.60 mmol) was dissolved in 30 mL of anhydrous dioxane. N,N'disuccinimide ester (12.0mmol) was dissolved in 20mL of anhydrous acetone, and added slowly under magnetic stirring. 12.0 mmol of 4-(dimethylamino)pyridine was dissolved in 20 mL of anhydrous acetone, and added slowly under magnetic stirring. After activation at room temperature for 6 hours, four-arm oxyethylene polyoxypropylene polyoxyethylene precipitates were obtained. [1]
[0044] 2. The preparation method of four-arm polyoxyethylene polyoxypropylene polyoxyethylene succinimide ester
[0045] Four-armed oxyethylene polyoxypropylene polyoxyethylene (0.60 mmol) was dissolved in 30 mL of anhydrous dioxane. N,N'disuccinimide ester (12.0mmol) was dissolved in 20mL of anhydrous acetone, and added slowly under ma...
Embodiment 1
[0052] Weigh 130mg four-arm oxyethylene polyoxypropylene polyoxyethylene succinimide ester (compound as shown in formula I, wherein, m is 56, n is 23, p is 11, x is 4, R 1 Amino) was dissolved in 1ml of normal saline to obtain solution 1 (mass-volume concentration of 130mg / ml), and 130mg of four-arm polyethylene glycol amino (as shown in formula VI, wherein m was 56, x was 4, R 2 Amino) was dissolved in 1ml of normal saline to obtain solution 2 (mass-volume concentration was 130mg / ml); use one of the double-barreled syringes to draw a mixed solution, then inject it into a cylindrical glass mold, and take it out after 30min , Use a universal tensile machine to measure the compressive strength, the strength can reach 3.2MPa, and the strength is high.
[0053] Degradation test: Prepare a cylindrical gel with a diameter of 1cm and a height of 1cm according to the above method, put the gel into a closed container with PBS buffer solution, and then put it in a constant temperature ...
Embodiment 2
[0058] Take by weighing 200mg four-arm polyacrylamide (as shown in formula II, wherein, m is 112, R 1 Amino) was dissolved in 1ml PBS solution to obtain solution 1 (mass-volume concentration is 200mg / ml), weighed 200mg four-arm polyoxyethylene polyoxypropylene polyoxyethylene succinimide ester (as shown in formula IV, where , m is 11, n is 112, p is 2, and x is 4) be dissolved in 1ml PBS to obtain solution 2 (mass-volume concentration is 200mg / ml); Then pour into the transparent sample bottle at the same time, invert the sample bottle, and the time when the liquid does not flow is the gelation time. The gel time of the system is 5s, the gelation speed is fast, and it can be quickly formed on the surface of the object.
[0059] The degradation performance of the hydrogel prepared in this example was tested according to the method in Example 1, and the degradation time was measured to be 3 days.
[0060] Combine this gel and Mestel quick-acting hemostatic powder to form a comp...
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