Ferroferric oxide modified biochar immobilized polycyclic aromatic hydrocarbon degrading bacterial agent as well as preparation and application thereof

A technology of triiron tetroxide and polycyclic aromatic hydrocarbons, which is applied in biochemical equipment and methods, fixed on or in inorganic carriers, microorganisms, etc., can solve the problem of the need to improve the degradation rate, achieve good degradation activity, and improve preservation. Effect of time and adhesion area increase

Pending Publication Date: 2021-08-24
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the degradation rate of the above-mentioned biochar-immobilized bacterial agents to phenanthrene and other bacterial species still needs to be improved.
[0006] Taken together, there is little use of Fe 3 o 4 Study on modified biochar immobilized polycyclic aromatic hydrocarbon degrading bacteria agent for PAH polluted environment remediation

Method used

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  • Ferroferric oxide modified biochar immobilized polycyclic aromatic hydrocarbon degrading bacterial agent as well as preparation and application thereof
  • Ferroferric oxide modified biochar immobilized polycyclic aromatic hydrocarbon degrading bacterial agent as well as preparation and application thereof
  • Ferroferric oxide modified biochar immobilized polycyclic aromatic hydrocarbon degrading bacterial agent as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Fe 3 o 4 Preparation of modified biochar

[0041] FeCl 3 and FeSO 4 ·7H 2 O is added in the beaker that deionized water is housed, stirs until dissolving completely, makes iron salt solution (wherein, component concentration is: 0.025mol / L FeCl 3 , 0.0125mol / L FeSO 4 ·7H 2 O). Mix 20g 30-80 mesh cotton stalk biochar with 1L iron salt solution and stir at about 65°C for 20min

[0042] A 10M NaOH solution was added until the pH was 11. After mixing for 1 hour, age at room temperature for 24 hours and filter. The remaining solid particles were washed repeatedly with deionized water and ethanol. Finally, the prepared biochar was dried in an oven. Made Fe 3 o 4 Modified biochar. Cotton stalk biochar, Fe 3 o 4 Scanning electron microscope image of modified biochar figure 1 As shown, it can be observed that the cotton stalk biochar has an obvious pore structure, the arrangement is regular, and the surface is relatively smooth and flat. 3 o 4 After modificati...

Embodiment 2

[0044] Fe 3 o 4 Preparation of modified biochar-immobilized polycyclic aromatic hydrocarbon degrading bacteria agent

[0045] Use an inoculation loop to pick a strain from the LB plate of AD-3 (Martelella sp.AD-3) and inoculate it in 5 mL of LB medium. After culturing for 48 hours, take out the Erlenmeyer flask and wash the cells repeatedly.

[0046] According to the volume ratio of 5% inoculum, take the expanded cultured bacterial solution and add it to a conical flask filled with 20mL 3% LB. The culture conditions are: rotation speed 180rpm, temperature 28°C, culture for 36h, centrifuge after the end, remove the supernatant . Resuspend in 20 mL 3% LB, OD 600 Adjust to 0.6 to obtain AD-3 bacterial liquid

[0047] Take by weighing the Fe prepared by 0.5g above-mentioned embodiment 1 3 o 4 The modified biochar was sterilized under high temperature and high pressure at 121°C for 20min, cooled to room temperature, added 20mL of AD-3 bacteria solution, placed in a constant t...

Embodiment 3

[0049] Fe 3 o 4 Degradation performance of modified biochar immobilized polycyclic aromatic hydrocarbon degrading bacteria agent

[0050] Adopt the Fe prepared by embodiment 2 3 o 4 The modified biochar-immobilized polycyclic aromatic hydrocarbon degrading bacteria agent was used in the PHE degradation experiment. The content of polycyclic aromatic hydrocarbons in the system was determined by high performance liquid chromatography (HPLC). Its degradation rate is as image 3 As shown, Fe 3 o 4 The degradation rate of 200 mg / L phenanthrene by the modified biochar-immobilized bacterial agent can reach 96.33% within 24 hours, while the degradation rate of phenanthrene by the cotton stalk biochar-immobilized bacterial agent is 84.51% under the same conditions, indicating that Fe 3 o 4 The modification improved the degradation effect of the biochar agent.

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Abstract

The invention relates to a ferroferric oxide modified biochar immobilized polycyclic aromatic hydrocarbon degrading bacterial agent as well as preparation and application thereof. The degrading bacterial agent comprises a Fe<3>O<4> modified biochar material and a bacterial strain loaded on the Fe<3>O<4> modified biochar material, the bacterial strain is moderately halophilic bacteria Martella sp.AD-3, the preservation number of the bacterial strain is CCTCC M 2011218, and the bacterial strain is preserved in the China Center for Type Culture Collection on June 30, 2011. By means of the invention, efficient degradation of polycyclic aromatic hydrocarbon can be achieved, the removal rate of 200 mg / L phenanthrene can reach 96.33% within 24 h, the removal rate is improved by 11.82% compared with the degradation rate of 84.51% of a biochar immobilized microbial agent, and the degradation rates of 74.18% and 90.00% on 200 mg / L phenanthrene within 24 h after the biochar immobilized microbial agent is stored at 20 DEG C and 4 DEG C for 21 d are achieved respectively. In actual contaminated soil, the bacillus subtilis still has efficient degradation activity, and when the addition amount of the bacillus subtilis is 3%, the 21d degradation rate of the bacillus subtilis to 87.2 mg / kg phenanthrene contaminated soil can reach 77.0%.

Description

technical field [0001] The invention belongs to the technical field of microbial bacterial agents, and relates to a ferric oxide modified biochar immobilized polycyclic aromatic hydrocarbon degradation bacterial agent and its preparation and application. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) are important persistent organic pollutants ubiquitous in the environment. They are emitted in large quantities and often exist in the soil for a long time and accumulate in large quantities, seriously endangering soil production and ecological functions. Its carcinogenic, teratogenic, and mutagenic properties seriously affect the environment and public health, and have been listed as priority pollutants by China, the United States and other countries. Oil extraction often causes PAHs pollution in the soil around oilfields, and the soil polluted by oil extraction is often salinized. Therefore, efficient and green remediation of soil PAHs pollution under hi...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N1/20B09C1/10C12R1/01
CPCC12N11/14C12N1/20B09C1/10
Inventor 崔长征沈佳敏沈辉陈欣傅展宇张峰单广波林匡飞
Owner EAST CHINA UNIV OF SCI & TECH
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