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Drug-loaded cell network-type growth-induced decellularized heart valve scaffold and preparation method thereof

A heart valve and inducing cell technology, applied in the field of biomedical materials, can solve problems such as weak adhesion, and achieve the effects of solving the phenomenon of sudden release, unstable drug release speed, and weak adhesion

Active Publication Date: 2022-03-22
WUHAN TEXTILE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

By arranging the drug-loaded polylactic acid / silk fibroin hollow fibers on one side of the decellularized heart valve, and suturing the drug-loaded polylactic acid / silk fibroin hollow fibers to the decellularized heart valve through the polylactic acid yarn, the cells were induced to move along the The yarn migrates to the side containing the drug, thereby inducing the cells to grow in a grid structure, achieving the purpose of fixing the cells on the surface of the decellularized heart valve, and solving the problem of weak adhesion of the cells on the surface of the decellularized heart valve to meet Practical application needs

Method used

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  • Drug-loaded cell network-type growth-induced decellularized heart valve scaffold and preparation method thereof
  • Drug-loaded cell network-type growth-induced decellularized heart valve scaffold and preparation method thereof
  • Drug-loaded cell network-type growth-induced decellularized heart valve scaffold and preparation method thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0035]The present invention also provides a preparation method of drug-loaded induced cell network growth decellularized heart valve stent, comprising the following steps:

[0036] S1. Dissolve polylactic acid in a solvent to prepare a polylactic acid solution with a predetermined concentration; add a predetermined amount of silk fibroin to the polylactic acid solution to obtain a polylactic acid / silk fibroin spinning solution, which is wet-spun through a skin core Silk obtained polylactic acid / silk fibroin hollow fiber;

[0037] S2. Cut the polylactic acid / silk fibroin hollow fiber obtained in step S1 into several sections, and seal one end of each polylactic acid / silk fibroin hollow fiber; The hollow interior of the polylactic acid / silk fibroin hollow fiber is injected with a drug solution, and then the open end is sealed to obtain several sections of drug-loaded polylactic acid / silk fibroin hollow fiber;

[0038] S3. Arrange the several pieces of drug-loaded polylactic aci...

Embodiment 1

[0043] This embodiment provides a drug-loaded acellular heart valve stent 100 induced by cell network growth, the schematic diagram of which is shown in figure 1 As shown, it includes a decellularized heart valve 10 and several sections of drug-loaded polylactic acid / silk fibroin hollow fibers 20 arranged in parallel on one side of the decellularized heart valve; the drug-loaded polylactic acid / silk fibroin hollow fibers 20 pass through The polylactic acid yarn 30 is sutured on the decellularized heart valve 10 , including the polylactic acid / silk fibroin hollow fiber 21 and the drug 22 encapsulated inside the polylactic acid / silk fibroin hollow fiber 21 .

[0044] This embodiment also provides a preparation method of the above drug-loaded acellular heart valve stent 100 induced by cell network growth, including the following steps:

[0045] S1. Preparation of polylactic acid / silk fibroin hollow fiber 21

[0046] Dissolving polylactic acid in the solvent 1,4-dioxane to prepar...

Embodiment 2～5

[0055] Examples 2 to 5 respectively provide a preparation method for drug-loaded cell-network-type growth decellularized heart valve stents. Compared with Example 1, the difference is that the process parameters in each step are changed. Each example The corresponding parameter values ​​are shown in Table 1, and the rest of the steps are consistent with those in Embodiment 1, and will not be repeated here.

[0056] The technological parameter value of table 1 embodiment 2~5

[0057]

[0058]

[0059] After testing, the drug-loaded cell-network-type growth decellularized heart valve stents prepared according to the above-mentioned process parameters respectively in Examples 2-5 have different drug release rates and cell growth rates, indicating that polylactic acid / silk fibroin can be adjusted Process parameters such as the content of silk fibroin in the hollow fiber and its preparation process, drug concentration, polylactic acid yarn fineness, and suture spacing can reg...

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Abstract

The invention provides a drug-loaded acellular heart valve support induced by cell grid growth and a preparation method thereof. The scaffold comprises a decellularized heart valve and a drug-loaded polylactic acid / silk fibroin hollow fiber arranged in parallel on one side of the decellularized heart valve. In the present invention, the polylactic acid / silk fibroin hollow fiber is firstly prepared by a skin-core wet spinning method, and the drug-loaded polylactic acid / silk fibroin hollow fiber is obtained after the drug is encapsulated inside; The silk fibroin hollow fiber is sutured on the decellularized heart valve to obtain the decellularized heart valve scaffold induced by drug-loaded cell grid growth. Through the above method, the present invention fully exerts the synergistic effect between drug slow release and induced cell growth technology, increases the drug content on one side of the decellularized heart valve through drug slow release, induces cells to grow to the side with high drug concentration, and makes cells It grows in a grid structure to solve the problem of weak adhesion of cells on the surface of decellularized heart valves.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a decellularized heart valve stent loaded with drugs to induce cell grid growth and a preparation method thereof. Background technique [0002] Heart valve disease is a common disease that threatens human health, and valve replacement is the main method of treatment. However, existing artificial heart valves still have defects: mechanical valves have good durability, but they are prone to coagulation after implantation in the body, and patients need to take anticoagulant drugs for life to reduce the risk of thrombosis; biological valves do not coagulate, but It is easy to calcify. After implantation in the body, the valve will calcify and decay after 10-15 years. On this basis, the development of tissue engineering provides a new way to find satisfactory artificial valves. The ideal tissue engineered heart valve is a new type of biological valve with cell activity ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/18A61L27/22A61L27/56D01D5/06D01D5/34D01D5/24
CPCA61L27/3625A61L27/227A61L27/18A61L27/56D01D5/06D01D5/34D01D5/24A61L2300/602C08L67/04C08L89/00
Inventor 杨红军刘昌俊庄燕顾绍金周应山徐卫林
Owner WUHAN TEXTILE UNIV
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