Visual rapid nucleic acid detection method of bovine nodular skin disease virus

A detection method and nodularity technology, which is applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., can solve the problems of high cost, insensitive detection method, long time, etc., and achieve specificity High efficiency, accurate and reliable detection results, and low detection cost
CN113308577AActive Publication Date: 2021-08-27HUAZHONG AGRI UNIV
5 Cites 1 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HUAZHONG AGRI UNIV
Publication Date
2021-08-27

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a visual rapid nucleic acid detection method of bovine nodular skin disease virus (LSDV), the method is characterized in that an RPA primer is designed for orf068 gene of bovine nodular skin disease virus, amplification is carried out, then Cas12a protein is added into an amplification product, enzyme digestion reaction is carried out, the reaction system comprises the Cas12a protein, sgRNA and a nucleic acid probe, and the signal emitted by the nucleic acid probe can be detected. The sensitivity for detecting orf068 plasmids is 5 copies / [mu] L, the sensitivity for detecting LSDV and goat pox virus (GTPV) is 0.1 TCID50 / [mu] L, the diagnostic sensitivity for detecting clinical samples is 96.3% (95% CI: 81.0%, 99.9%), the specificity is high, the changes in color are observed by eyes for result judgement, and the method has the advantages of simplicity, convenience, rapidness, suitability for field detection and the like.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the field of virus detection, and in particular relates to a nucleic acid visualization detection technology mediated by a CRISPR-Cas12a system, a method and a kit for rapidly detecting bovine nodular skin disease (LSD). Background technique

[0002] Lumpy skin disease (LSD) is a skin disease caused by Lumpy skin disease virus (LSDV), characterized by fever, skin edema and local hard nodules. Will seriously hinder the development of the cattle industry. LSD can cause huge financial losses. For example, the milk production of dairy cows is reduced, the growth performance of beef cattle is reduced, the bulls are temporarily or permanently sterile, and the utilization value of hides is destroyed. The incidence rate of the disease is between 2% and 45%, and the fatality rate is as high as 20%. Dairy cows are more susceptible and have been listed as one of the diseases that must be notified by the World Organization for Animal Hea...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More