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Method for improving efficiency of treating harmful algal blooms by clay based on microbial compound modification

A compound modification and microbial technology, applied in biological water/sewage treatment, chemical instruments and methods, water/sludge/sewage treatment, etc., to achieve good application prospects, strengthen bridging effect, and enhance removal ability

Active Publication Date: 2021-08-31
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] It can be seen that among the above-mentioned harmful algal bloom control methods, the modified clay method exhibits the characteristics of quick effect, low dosage, and safety and reliability, but it may require a natural recovery process of the ecosystem after large-scale disposal actions; biological methods Although the emergency response is poor, this method has certain advantages in ecological regulation when dealing with harmful algal blooms

Method used

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  • Method for improving efficiency of treating harmful algal blooms by clay based on microbial compound modification
  • Method for improving efficiency of treating harmful algal blooms by clay based on microbial compound modification
  • Method for improving efficiency of treating harmful algal blooms by clay based on microbial compound modification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Weigh 7 parts, each part of 0.5g kaolin and 0.1g polyaluminium chloride powder, mix well, sterilize by high-temperature steam at 121°C for 30min, dry in an oven at 80°C for 1h, sterilize under ultraviolet light for 20min, cool To room temperature, 7 parts of type I modified clay are obtained, which are ready for use;

[0025] Dilute the EM bacteria solution with sterile seawater into mother solutions with the same volume and different bacterial densities, and add the above-mentioned Type I modified clay to it, so that each gram of modified clay is respectively equivalent to 0, 10 10 , 2×10 10 , 5×10 10 、10 11 , 1.5×10 11 , 2×10 11 A microbial compound was obtained to obtain a type I microbial compound modified clay stock solution, which was shaken and placed in a constant temperature shaking incubator at 30°C, and matured for 2 hours at an oscillation frequency of 150r / min to obtain a microbial compound modified clay system.

[0026] Among them, the EM bacterial li...

Embodiment 2

[0030] The above microbial composite modified clay system was centrifuged (8000r / min, 3min), the supernatant was discarded and the sample was completely submerged in 5% glutaraldehyde for 1h. Rinse and soak the samples with 0.1 mol / L phosphate buffer at room temperature for 3 times (10 min each time), dehydrate them with ethanol gradient, and replace them with isoamyl acetate. After drying in a critical point dryer for 3 hours, the samples were fixed with conductive adhesive and sprayed with gold. A scanning electron microscope (S-3400N, Hitachi, JP) was used to observe the solidification of microorganisms on the composite modified clay surface. Such as figure 2 As shown, microorganisms can immobilize on the modified clay surface.

Embodiment 3

[0032] Prepare microbial composite modified clay system as described in embodiment 1, make every gram of clay solidify 5 * 10 10 After mixing, shake them well and place them in constant temperature shaking incubators at 4°C, 20°C, and 60°C, respectively, and ripen them at each temperature for 4h, 48h, 72h, and 96h at an oscillation frequency of 150r / min, that is, they can be obtained at different temperatures. Microbial compound modified clay system with different curing time.

[0033] Take the algae liquid of Prorocentrum donghaiense in the exponential growth phase (algae cell density is about 1×10 8cell / L) to a 50mL colorimetric tube, add the microbial composite modified clay stock solution that was matured at different temperatures for different times in the algae liquid, so that the final concentration is 0.1g / L. Shake well, let it stand for 24 hours, and calculate its algae removal efficiency (see image 3 ). The results showed that within the appropriate range, the lo...

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Abstract

The invention belongs to the technical field of prevention and control of harmful algal blooms, and particularly relates to a method for improving the efficiency of treating harmful algal blooms by clay based on microbial compound modification. Some microorganisms are gathered and solidified on the surface of clay (or modified clay) according to a certain proportion under a certain condition, the local concentration of the microorganisms and the algae removal characteristic of the surface of the clay (or modified clay) are increased, the inhibition effect of the microorganisms and the clay (or modified clay) on microalgae is enhanced, and a composite modified clay system with the 1 + 1 > 2 effect is formed. The method has the advantages that the algae removal efficiency of the original clay or the modified clay can be improved, the decomposition of algal blooms and other organic matters in water can be effectively accelerated, and the effect of further purifying water is achieved.

Description

technical field [0001] The invention belongs to the technical field of prevention and control of harmful algal blooms, and specifically relates to a method for improving the efficiency of clay in controlling harmful algal blooms based on microbial compound modification. Background technique [0002] Harmful algal bloom is an ecological disaster phenomenon in which the abnormal proliferation or accumulation of algae in seawater or freshwater causes discoloration of a large area of ​​water and produces toxic or harmful effects on fish, birds, shellfish, shrimp, marine mammals and humans. In recent years, harmful algal blooms have occurred frequently in my country's coastal waters, and the scope of influence has been expanding, seriously endangering the coastal ecological environment and aquaculture industry. Effective control methods that can be applied on a large scale are urgently needed. [0003] Theoretically, there are many ways to control red tides, such as physical, che...

Claims

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Application Information

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IPC IPC(8): C02F3/34C12N11/14
CPCC02F3/34C02F3/347C12N11/14
Inventor 俞志明刘姗姗曹西华宋秀贤袁涌铨贺立燕吴在兴
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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