Method for improving efficiency of treating harmful algal blooms by clay based on microbial compound modification

A compound modification and microbial technology, applied in biological water/sewage treatment, chemical instruments and methods, water/sludge/sewage treatment, etc., to achieve good application prospects, strengthen bridging effect, and enhance removal ability

Active Publication Date: 2021-08-31
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] It can be seen that among the above-mentioned harmful algal bloom control methods, the modified clay method exhibits the characteristics of quick effect, low dosage, and safety and reliability, but it may require a natural

Method used

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  • Method for improving efficiency of treating harmful algal blooms by clay based on microbial compound modification
  • Method for improving efficiency of treating harmful algal blooms by clay based on microbial compound modification
  • Method for improving efficiency of treating harmful algal blooms by clay based on microbial compound modification

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] Example 1

[0024] Weigh parts 7, each PAC 0.1g 0.5g kaolin powder, after mixing by 121 ℃ autoclavble 30min after oven drying at 80 ℃ IH, placed under UV light sterilization 20min, cooled to room temperature to obtain 7 parts of I-type modified clays, stand;

[0025] The EM were diluted with the same volume of sterile water, different bacterial density of the mother liquor, to which the above-described modified clay type I, per gram of the modified clay respectively 0,10 10 2 × 10 10 , 5 × 10 10 10 11 , 1.5 × 10 11 2 × 10 11 Microbial compound, to obtain modified clay composite microorganism type I stock solution, shake deg.] C thermostat 30 placed in the shaker-incubator to the oscillation frequency of 150r / min in aged 2h, i.e. to obtain modified clay complex microbial system.

[0026] Which, EM bacilli purchased from Wuhan Tian Chen Biotechnology Co., Ltd., its microbiological content of 10 10 cells / mL, the number of microorganisms according to components percentage: ...

Example Embodiment

[0029] Example 2

[0030] The modified clay composite system of the microorganisms by centrifugation (8000r / min, 3min), the supernatant was discarded and the sample was completely immersed in 5% glutaraldehyde fixation 1h. After using 0.1mol / L phosphate buffer at room temperature the sample was immersed rinsed 3 times (10min), dehydrated ethanol gradient, which is to be replaced in isoamyl acetate. After critical point drying apparatus was placed in a drying process 3h, samples were fixed with a conductive adhesive and coated with gold. Observation cured composite modification are microorganisms on the surface of the clay using a scanning electron microscope (S-3400N, Hitachi, JP). like figure 2 , The microorganism may be cured in the surface modified clay.

Example Embodiment

[0031] Example 3

[0032] The microorganism is formulated as a modified clay composite system of embodiment, per gram of clay on the cured 5 × 10 10 Microorganisms, are placed in shaking after mixing 4 ℃, 20 ℃, 60 ℃ thermostatic shaking incubator to an oscillation frequency at each temperature of min 150r / maturation respectively 4h, 48h, 72h, 96h, i.e., at different temperatures to give different microorganisms modified clay composite curing system time.

[0033] (Cell density donghaiense algae solution taken in the exponential growth phase of about 1 × 10 8Cell / L) to 50 ml of colorimetric tubes, which were added to different times in algae to obtain microbial composite modified clay reserves for different times at different temperatures, respectively, and the final concentration was 0.1 g / L. Shake well, stand for 24 hours, calculate its algae efficiency (see image 3 ). The results show that the longer the mood time of microbial composite modified clay is, the higher the rip...

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Abstract

The invention belongs to the technical field of prevention and control of harmful algal blooms, and particularly relates to a method for improving the efficiency of treating harmful algal blooms by clay based on microbial compound modification. Some microorganisms are gathered and solidified on the surface of clay (or modified clay) according to a certain proportion under a certain condition, the local concentration of the microorganisms and the algae removal characteristic of the surface of the clay (or modified clay) are increased, the inhibition effect of the microorganisms and the clay (or modified clay) on microalgae is enhanced, and a composite modified clay system with the 1 + 1 > 2 effect is formed. The method has the advantages that the algae removal efficiency of the original clay or the modified clay can be improved, the decomposition of algal blooms and other organic matters in water can be effectively accelerated, and the effect of further purifying water is achieved.

Description

technical field [0001] The invention belongs to the technical field of prevention and control of harmful algal blooms, and specifically relates to a method for improving the efficiency of clay in controlling harmful algal blooms based on microbial compound modification. Background technique [0002] Harmful algal bloom is an ecological disaster phenomenon in which the abnormal proliferation or accumulation of algae in seawater or freshwater causes discoloration of a large area of ​​water and produces toxic or harmful effects on fish, birds, shellfish, shrimp, marine mammals and humans. In recent years, harmful algal blooms have occurred frequently in my country's coastal waters, and the scope of influence has been expanding, seriously endangering the coastal ecological environment and aquaculture industry. Effective control methods that can be applied on a large scale are urgently needed. [0003] Theoretically, there are many ways to control red tides, such as physical, che...

Claims

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Application Information

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IPC IPC(8): C02F3/34C12N11/14
CPCC02F3/34C02F3/347C12N11/14
Inventor 俞志明刘姗姗曹西华宋秀贤袁涌铨贺立燕吴在兴
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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