Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for continuously and rapidly measuring concentration of ammonia nitrogen in fresh water in small system

A technology for rapid determination of ammonia nitrogen concentration, applied in color/spectral characteristic measurement, material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions, etc., can solve the toxicity and workload of Nessler's reagent Large size, large amount of waste liquid, etc., to achieve the effect of reducing usage, reducing measurement cost, and reducing waste liquid volume

Inactive Publication Date: 2021-08-31
GUANGDONG UNIV OF PETROCHEMICAL TECH
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the national standard method needs to clean a large number of glass instruments, which requires a lot of work, takes a long time, and a large amount of waste liquid. The Nessler reagent used is toxic and easily pollutes the environment.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for continuously and rapidly measuring concentration of ammonia nitrogen in fresh water in small system
  • Method for continuously and rapidly measuring concentration of ammonia nitrogen in fresh water in small system
  • Method for continuously and rapidly measuring concentration of ammonia nitrogen in fresh water in small system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] This embodiment provides a method for continuously and rapidly measuring the concentration of ammonia nitrogen in fresh water with a small system, comprising the following steps:

[0056] S1: Standard working curve formulation:

[0057]S1.1: Add ammonia nitrogen standard working solution 0, 10, 20, 40, 80, 120, 160, 200 μL to 8 round-bottomed 2 mL centrifuge tubes, dilute to 1 mL with ultrapure water, and obtain ammonia nitrogen concentrations of 0.0, 0.1, 0.2, 0.4, 0.8, 1.2, 1.6, 2.0μg·mL -1 1mL each of ammonia nitrogen working solution.

[0058] S1.2: Add 10-25 μL to each ammonia-nitrogen working solution to a concentration of 500 g·L -1 Potassium sodium tartrate solution after mixing;

[0059] S1.3: Add Nessler's reagent with sodium potassium tartrate solution to each ammonia nitrogen working solution, mix well and let stand for 10-30 minutes;

[0060] S1.4: After the color is stable, use a microplate reader to measure and record the absorbance of each ammonia-ni...

Embodiment 2

[0077] 1. Experimental design

[0078] 1.1. Optimal experiment of small system determination of influencing factors of ammonia nitrogen

[0079] In order to accurately establish a method for the determination of ammonia nitrogen in a small system and optimize the best reaction conditions for the improved method, the effects of the amount of chromogen, color development time, salinity, pH, etc. on the reaction process of ammonia nitrogen determination were explored:

[0080] ① Factors affecting the amount of developer. At room temperature, the preparation concentration is 0.0, 0.1, 0.2, 0.4, 0.8, 1.2, 1.6, 2.0μg·mL -1 For ammonia nitrogen working solution, set four groups for each concentration of ammonia nitrogen, two parallels for each group, add potassium sodium tartrate 10 μL, 15 μL, 20 μL, 25 μL and Nessler’s reagent 10 μL, 15 μL, 20 μL, 25 μL to the ammonia nitrogen working solution of each concentration for color development Reaction, in order to determine the optimal ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for continuously and rapidly measuring the concentration of ammonia nitrogen in fresh water in a small system. The method comprises the steps: preparing 1 mL of ammonia nitrogen working solutions with different ammonia nitrogen concentrations respectively; adding 10-25 [mu]L of a potassium sodium tartrate solution into each ammonia nitrogen working solution, and uniformly mixing; adding a Nessler reagent of which the volume is equal to that of the potassium sodium tartrate solution into each ammonia nitrogen working solution, uniformly mixing and standing for 10-30 minutes; after the color is stable, measuring and recording the absorbance of each ammonia nitrogen working solution by taking a blank as a reference; drawing a standard working curve, and solving a regression equation of the standard working curve; taking 1mL of a water sample, measuring and recording the absorbance of the water sample according to the same steps as the ammonia nitrogen working solution; replacing the water sample with water, and determining the absorbance of the blank test according to the same steps as the water sample determination; and calculating a result. Compared with an ammonia nitrogen national standard determination method, the method for continuously and rapidly determining the concentration of ammonia nitrogen in the small system has the advantages of high working efficiency, low determination cost, reduced secondary pollution and large ammonia nitrogen concentration determination range.

Description

technical field [0001] The invention specifically relates to a method for continuously and rapidly measuring the concentration of ammonia nitrogen in fresh water with a small system. Background technique [0002] The form of ammonia nitrogen in water is free ammonia (NH 3 ) or ammonium ion (NH 4 + ), is one of the important indicators for surface water and sewage monitoring, and also one of the main monitoring indicators for the country to implement energy conservation, emission reduction and pollution control. There are two sources of ammonia nitrogen in water bodies: on the one hand, domestic sewage containing various protein organic matter undergoes complex biochemical reactions under the action of microorganisms, and decomposes to produce NH 3 ; On the other hand, nitrogen-containing wastewater is produced in the process of industrial production and agricultural production, such as the production of synthetic ammonia fertilizer plants and agricultural fertilization. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/31G01N21/78
CPCG01N21/31G01N21/78
Inventor 孙巍
Owner GUANGDONG UNIV OF PETROCHEMICAL TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com