Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Gordonia sp. for producing long carbon chain mycolic acid and application of Gordonia sp.

A Gordonia and mycolic acid technology applied in the field of desulfurization microorganisms to achieve high hydrophobicity, improve desulfurization efficiency, and solve the problem of mass transfer efficiency

Active Publication Date: 2021-09-03
SHANDONG UNIV OF SCI & TECH
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The prior art shows that the desulfurization product 2-HBP is highly lethal to the desulfurization bacteria, and the prior art report adopts the method of adding additional MgSO 4 Sulfur source or glucose is used as carbon source to improve the resistance of bacteria to 2-HBP, but there are relatively few studies on desulfurization bacteria that are resistant to the poisoning effect of desulfurization product 2-HBP

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Gordonia sp. for producing long carbon chain mycolic acid and application of Gordonia sp.
  • Gordonia sp. for producing long carbon chain mycolic acid and application of Gordonia sp.
  • Gordonia sp. for producing long carbon chain mycolic acid and application of Gordonia sp.

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Isolation, purification and identification of embodiment 1 bacterial strain

[0024] Sample acquisition: On April 10, 2016, the oil-contaminated soil of Shengli Oilfield was collected from Dongying, Shandong.

[0025] Isolation and purification of strains:

[0026] Using 4-methyldibenzothiophene (4-MDBT) and 3-methylbenzothiophene (3-MBT) as the sole sulfur source, screened from the oil-contaminated soil of Shengli Oilfield to specifically break carbon -Strains desulfurized by means of sulfur bonds. Specific steps:

[0027] ① Take an appropriate amount of soil sample and add it to a test tube containing sterile deionized water, shake it fully and let it stand for 10 minutes, transfer an appropriate amount of the upper layer liquid to 50 mL liquid selective medium (with 4-MDBT and 3-MBT as the only sulfur source ), cultivated for 4 days at 30°C and 160rpm;

[0028] ②Take an appropriate amount of culture medium from step ① and transfer it to 50ml liquid LB medium, and...

Embodiment 2

[0057] Example 2 Hydrophobicity and mycolic acid production of Gordonia sp.JT-2 bacterial strain

[0058] Gordonia sp.JT-2 cell surface structure:

[0059] Scanning electron microscopy to observe the surface morphology of bacteria, such as figure 2 As shown, it was found that the surface of the cell wall of Gordonia sp.JT-2 was uneven and wrapped by a layer of waxy substance. By extracting the waxy substance on the surface of the cell wall, it was analyzed by ESI-high resolution mass spectrometry (ESI-HRMS) ( figure 2 ), m / z=851.57680 is mycolic acid with four unsaturated double bonds and a total carbon number of 58. Mycolic acid is a fatty acid containing α-alkyl chain-β-hydroxyl. Bacteria are wrapped in mycolic acid and adhere to each other to form aggregates. This form of aggregation is conducive to resisting the oil phase in the oil-water two-phase reaction system. The toxic effect of solvent, meanwhile, the presence of mycolic acid and its carbon chain length are rela...

Embodiment 3

[0073] Example 3 Effect of hydroxylated desulfurization products on the growth and desulfurization effect of Gordonia sp.JT-2 under different systems

[0074] During the biological desulfurization process, the hydroxylated desulfurization products will inhibit the desulfurization activity and growth of desulfurization bacteria (poisonous effect). Biological desulfurization was carried out in water phase system and oil-water multiphase system, and the effect of hydroxylated desulfurization products on the biological desulfurization efficiency of Gordonia sp.JT-2 in different systems was studied.

[0075] Aqueous phase system: Add DBT as the only sulfur source in the sulfur-free medium (the concentration of DBT in the medium is 0.543mmol / L) and add different concentrations of hydroxylated desulfurization products (2-hydroxybiphenyl) to carry out biological desulfurization experiments;

[0076] Oil-water multiphase system: add DBT-simulated oil (DBT is dissolved in n-tetradecane,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
contact angleaaaaaaaaaa
Login to View More

Abstract

The invention discloses Gordonia sp. for producing long carbon chain mycolic acid and an application of the Gordonia sp., and belongs to the technical field of desulfurization microorganisms. The Gordonia sp. for producing the long carbon chain mycolic acid is Gordonia sp. JT-2, and the preservation number of the Gordonia sp. JT-2 is CCTCC CNO: M 2021493. The Gordonia sp.JT-2 disclosed by the invention has strong hydrophobicity, and when the Gordonia sp.JT-2 is used for desulfurizing an actual oil product, the Gordonia sp.JT-2 can secrete more mycolic acid, so that the Gordonia sp.JT-2 has stronger hydrophobicity. Therefore, the characteristic of extremely high hydrophobicity of the Gordonia sp.JT-2 in an oil-water heterogeneous system can be utilized, the toxic action of hydroxylation desulfurization products on bacteria is reduced, besides, the problem of low oil-water mass transfer efficiency of sulfur-containing organic matters is solved, and the desulfurization efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of desulfurization microorganisms, and in particular relates to a Gordonia strain producing long carbon chain mycolic acids and an application thereof. Background technique [0002] Petroleum is one of the main energy sources in the world today and plays an indispensable role in the development of the national economy. There are various forms of sulfur-containing organic compounds in petroleum, which not only reduce the quality of oil products, but also release a large amount of sulfur oxides (SO x ), thus having a huge impact on the environment. In order to control this adverse effect, environmental protection agencies in many countries have proposed increasingly strict legislation on the total sulfur content of oil products. At present, the latest proposals from my country, the United States, the European Union, Japan and other countries set the sulfur content standard in oil products as 10ppm (the standa...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C10G32/00C12R1/01
CPCC12N1/20C10G32/00
Inventor 陈水泉臧萌李琳孙烁
Owner SHANDONG UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com