Comparative transcriptome analysis method for peanut leaf gene differential expression under intercropping corn

A technology of transcriptome analysis and gene difference, applied in biochemical equipment and methods, sequence analysis, microbial measurement/inspection, etc., can solve problems such as lack of research, unexplored and analyzed, and impact on dwarf crop productivity, and achieve Method Accurate Effect
CN113337633AActive Publication Date: 2021-09-03GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
Publication Date
2021-09-03

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Abstract

The invention relates to the technical field of biology, and particularly relates to a comparative transcriptome analysis method for peanut leaf gene differential expression under intercropping corn. The method comprises the steps that an RNA-seq technology is utilized for comparing and analyzing differential expression genes of peanut functional leaves in the pod expansion period under peanut single cropping and intercropping corn stress; differential genes related to metabolic pathways are taken as main genes, important genes and key metabolic pathways formed by peanut pod yield under intercropping corn stress are excavated, and a theoretical basis is provided for reducing adverse effects of intercropping corn stress on peanut yield by means of a directional chemical regulation and control technology; and the relative expression level of candidate differential expression genes is verified through qRT-PCR detection, and finally the qRT-PCR detection result is roughly consistent with multiple changes in transcriptome sequencing analysis. The transcriptome sequencing analysis method is accurate, feasible, effective and simple.
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Description

【Technical field】

[0001] The invention relates to the field of biotechnology, in particular to a comparative transcriptome analysis method for the differential expression of genes in peanut leaves under intercropping corn. 【Background technique】

[0002] Peanut is an important oil crop and economic crop in my country. Peanut intercropping is a green, high-efficiency and high-yield planting mode widely used in time, space and ecology, and the intercropping cultivation mode between different crops is very rich. Among them, the intercropping of corn and peanuts has incomparable advantages over traditional single cropping. The growth and development of corn and peanuts are complementary in time and space. Intercropping between the two can not only change the ecological structure of the farmland, but also make full use of light, temperature, water, heat and nutrients and other resources, improve the high-efficiency utilization ability of maize to strong light and peanut to low li...

Claims

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