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Preparation method of needle point positioning enrichment type surface enhanced Raman scattering substrate

A surface-enhanced Raman, enrichment technology, applied in Raman scattering, material excitation analysis, instrumentation, etc., can solve the problems of complex micromachining and surface treatment, hindering the self-assembly of nanoparticles, and hot spot blocking Raman spectroscopy, etc. Achieve the effect of facilitating rapid on-site detection, reducing experimental costs, and reducing experimental cycles

Active Publication Date: 2021-09-03
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the diffusion-limited transport process, it is very challenging to efficiently transport low-concentration sub-nanomolar levels of analytes to hot spots on SERS substrates in a relatively short period of time.
How to concentrate a large amount of analyte solution in a short time and precisely confine the analyte to a small-sized sensitive "hot spot" area remains a great challenge
At the same time, due to the contact line pinning phenomenon during the droplet drying process, this phenomenon hinders the self-assembly of nanoparticles.
At present, the most common method is to use a superhydrophobic SERS substrate composed of plasmonic nanostructures with a hydrophobic coating, but the hydrophobic nanocoating is likely to cause local blockage of hot spots and Raman spectrum interference, etc., and the microfabrication and surface treatment are more complicated.

Method used

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  • Preparation method of needle point positioning enrichment type surface enhanced Raman scattering substrate
  • Preparation method of needle point positioning enrichment type surface enhanced Raman scattering substrate
  • Preparation method of needle point positioning enrichment type surface enhanced Raman scattering substrate

Examples

Experimental program
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preparation example Construction

[0037] see figure 1 , a method for preparing a needle-tip-enriched surface-enhanced Raman scattering substrate of the present invention includes the following process: using a metal sheet with a higher thermal conductivity as a heat-conducting gasket, and fixing the tip of an acupuncture steel needle at a certain distance from the heat-conducting gasket, Under high temperature conditions, based on the Leidenfrost effect, the liquid droplets containing the gold nanoparticle colloidal solution and the molecules to be tested are suspended on the thermal pad, and the specific position is anchored by the needle tip. As the solvent evaporates, the droplet gradually detaches from the surface of the thermal pad and adsorbs to the needle tip, where gold nanoparticles and analyte molecules are concentrated and enriched for rapid detection of micro-Raman and handheld Raman.

[0038] A method for preparing a needle-tip-positioned enriched surface-enhanced Raman scattering substrate, using...

Embodiment 1

[0053] 1) Clean the Al sheet and needle tip: Soak the Al sheet in acetone solution for 10 minutes and ultrasonically clean it for 10 minutes, then use absolute ethanol and deionized water for 10 minutes, and dry it at 60°C for 30 minutes before use. The cleaning process of acupuncture steel needles is the same as that of Al sheets.

[0054] 2) Put the Al sheet that has been cleaned and has a flat surface on a heating table, and after heating to 300°C, the Al sheet is fully heated and evenly heated, and kept warm for use. The acupuncture steel needles were hung vertically on the Al sheet, and the distance between the needle tip and the Al sheet was about 0.5 cm.

[0055] 3) the volume is 0.1mL, the size is 100nm and the solution (solvent is ethanol) of the gold nanoparticle colloid that concentration concentrates 2 times, and the crystal violet dye molecular solution (solvent is ethanol) that volume is 0.1mL mixes, mixes The droplet is dropped on the tip of the needle, and the...

Embodiment 2

[0057] 1) Clean the Al sheet and needle tip: Soak the Al sheet in acetone solution for 10 minutes and ultrasonically clean it for 10 minutes, then use absolute ethanol and deionized water for 10 minutes, and dry it at 60°C for 30 minutes before use. The cleaning process of acupuncture steel needles is the same as that of Al sheets.

[0058] 2) Put the Al sheet that has been cleaned and has a flat surface on a heating table, and after heating to 300°C, the Al sheet is fully heated and evenly heated, and kept warm for use. The acupuncture steel needles were hung vertically on the Al sheet, and the distance between the needle tip and the Al sheet was about 0.5 cm.

[0059] 3) The volume is 0.1mL, the size is 50nm and the solution (solvent is ethanol) of gold nanoparticle colloid that concentration concentrates 2 times, and the crystal violet dye molecule solution (solvent is ethanol) that volume is 0.1mL mixes, mixes The droplet is dropped on the tip of the needle, and the dropl...

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Abstract

The invention discloses a preparation method of a needle point positioning enrichment type surface-enhanced Raman scattering substrate, which comprises the following steps: heating a heat-conducting gasket, fixing a needle point above the heat-conducting gasket, arranging a gap between the needle point and the heat-conducting gasket, dropwise adding a gold nanoparticle solution and a to-be-detected molecular solution at the needle point, enabling liquid drops of a gold nanoparticle solution and a to-be-detected molecule solution to suspend on the heat-conducting gasket, along with solvent evaporation of the gold nanoparticle solution and the to-be-detected molecule solution, gradually separating the liquid drops from the surface of the heat-conducting gasket and adsorbed to the needle tip, concentrating and enriching gold nanoparticles and to-be-detected molecules at the needle tip, and obtaining the needle tip enriched surface enhanced Raman scattering substrate. The to-be-detected molecules and the enhanced particles are enriched at the needle point through high-temperature driving, so that the sensitivity and repeatability of SERS detection are effectively improved.

Description

technical field [0001] The invention belongs to the technical field of surface-enhanced Raman scattering spectroscopy, in particular to a method for preparing a needle point-positioned enrichment surface-enhanced Raman scattering substrate. Background technique [0002] Surface-enhanced Raman spectroscopy (SERS) is an ultrasensitive molecular detection technique developed in recent decades for surface-enhanced (bio)chemical sensing and analysis. SERS detection involves the interaction between plasmonic nanostructures, analyte molecules and laser light. By designing a novel plasmonic nanostructure, increasing the laser intensity and the inelastic Raman scattering rate of the analyte molecule in the hotspot area, the SERS enhancement factor is greater than 10 7 times. SERS research can achieve rapid and high-sensitivity detection of analytes at extremely low concentrations, and can be applied to point-of-care biomedical diagnosis, food and water quality analysis, and environ...

Claims

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Application Information

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IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 尤红军方吉祥张瑞元于亮
Owner XI AN JIAOTONG UNIV
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