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Thin-layer chromatography detection method of abelmoschus manihot medicinal material, extract or preparation

A detection method, the technology of hollyhock flowers, is applied in the direction of measuring devices, material separation, and analysis of materials, which can solve the problems of poor inspection effect and few detection components.

Active Publication Date: 2021-09-14
JIANGSU SUZHONG PHARM GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, the 2020 edition of "Chinese Pharmacopoeia" contains only the quality standards of hollyhock flowers and huangkui capsules for TLC identification of quercetin; Zhang Yu et al. are studying the TLC identification of flavonoids Two flavonoid components rutin and quercetin were identified by the same TLC method, but the detected components were few and the inspection effect was not good; the patent CN103487546B disclosed a rutin, hyperin, quercetin Cortex as the reference substance or rutin and hyperin as the reference substance, using glacial acetic acid as the developer, the method for identifying the medicinal materials of hollyhock flowers, and its detection components are also less

Method used

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  • Thin-layer chromatography detection method of abelmoschus manihot medicinal material, extract or preparation
  • Thin-layer chromatography detection method of abelmoschus manihot medicinal material, extract or preparation
  • Thin-layer chromatography detection method of abelmoschus manihot medicinal material, extract or preparation

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Effect test

preparation example Construction

[0053] (1) Preparation of hollyhock flower extract granules

[0054] Take the dried extract of marshmallow flowers prepared in the above 1.2 (1), crush it through an 80-mesh sieve, take 500 g of the extract, add 550 g of mannitol and 4 g of magnesium stearate, dry granulate, and make granules to obtain final product.

[0055] (2) Preparation of the test sample solution of hollyhock flower granule

[0056] Take the hollyhock flower granule preparation equivalent to 4g of hollyhock flower medicinal material, make 10g of aqueous solution, stir and mix well, add ethyl acetate to extract 3 times (3:1:1), combine the extracts, and dilute to 50mL with ethyl acetate bottle, as the preparation test solution.

[0057] Preparation of pretreatment step 2 reference substance solution

[0058] 2.1 Preparation of a single reference substance solution: Take appropriate amounts of rutin, hyperin, isoquercetin, cottoncetin-8-O-β-D-glucuronide, myricetin and quercetin respectively, and add 70 ...

Embodiment 1

[0061] Get 2 μ L of the hollyhock flower granule preparation need testing solution prepared in pretreatment step 1.3, 10 μ L of the mixed reference substance solution prepared in pretreatment step 2.2, respectively spot on the same polyamide film, with toluene-acetone-methanol (2:1:7 ) as developer, unfold, take out, dry in the air, spray with aluminum trichloride color developer until the spots are clearly colored, and inspect under a 365nm ultraviolet lamp. Test results such as figure 1 As shown, the spots are clearly separated and well separated.

Embodiment 2

[0063] Get 2 μ L of the hollyhock flower granule preparation test solution prepared in pretreatment step 1.3, and 10 μ L of the mixed reference substance solution prepared in pretreatment step 2.2, and place them on the same polyamide film respectively, with toluene-acetone-methanol (1:1:8 ) as developer, unfold, take out, dry in the air, spray with aluminum trichloride color developer until the spots are clearly colored, and inspect under a 365nm ultraviolet lamp. Test results such as figure 2 As shown, the spots are clearly separated and well separated.

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Abstract

The invention relates to the technical field of medicine quality control, in particular to a thin-layer chromatography detection method of an abelmoschus manihot medicinal material, extract or preparation. The method comprises the following steps of respectively dripping any test solution and reference solution of the abelmoschus manihot medicinal material, extract or preparation on the same thin-layer plate, developing by using toluene-acetone-methanol as a developing solvent, taking out, airing, spraying a color developing agent until spots are clearly developed, and inspecting under an ultraviolet lamp of 365 nm. Compared with the prior art, the method has the advantages that the rutin, the gossypotin-8-O-beta-D-glucuronide, the myricetin and the quercetin can be simultaneously identified, the abelmoschus manihot medicinal material, the extract, the preparation or the composition containing the four flavonoid components can be quickly subjected to qualitative and quantitative analysis, the operation is simple and convenient, and the reproducibility is good.

Description

technical field [0001] The invention belongs to the technical field of medicine quality control, and in particular relates to a thin-layer chromatographic detection method of marshmallow medicinal materials, extracts or preparations. Background technique [0002] Hollyhock [Abelmoschusmanihot (L.) Medicus] is a plant of the genus Okra in the Malvaceae family. It was first recorded in "Jiayou Materia Medica", "The spring-growing seedling leaves are similar to hollyhock, but the leaf tips are narrow and notched, and the flowers are light yellow in late summer. ". Hollyhock is widely distributed and rich in resources. "Compendium of Materia Medica" records: "Its flowers are sweet, cold, slippery, and non-toxic. Need medicine for sores, eliminate gangrene, soak in oil and apply soup to burns." Studies have shown that the total flavonoids of marshmallow flowers mainly include the following 10 flavonols and their glycosides: gossetin-8-0-β-D-glucuronide, gossetin-3'-O-glucoside,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90G01N30/94G01N30/95G01N1/40
CPCG01N30/90G01N30/94G01N30/95G01N1/40
Inventor 唐海涛周兆玲王殿广葛海涛徐杰
Owner JIANGSU SUZHONG PHARM GRP CO LTD