A kind of hemostatic material and preparation method thereof
A hemostatic material and animal tissue technology, applied in the field of medical materials, can solve the problems of increased cytotoxicity, improved effect, complicated preparation process, etc., and achieve the effect of simple preparation process, short time consumption, and wide sources
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[0033] The preparation of decellularized tissues and organs involves the removal of cells and genetic material from the tissue or organ, leaving the complex ultrastructural extracellular matrix (ECM) as a natural scaffold. The techniques currently used in decellularization include physical, chemical and biological enzymatic methods, each of which has advantages and disadvantages. Two important criteria to be met during decellularization are: (i) preservation of native structures and (ii) removal of maximal cellular components. The choice of decellularization agent depends on the nature of the tissue or organ. The present invention has no special requirements for the method of decellularization, which can be carried out by using existing decellularization methods. The available decellularization methods include but are not limited to physical methods including freezing and thawing, direct pressure, electroporation, ultrasound and stirring; chemical methods mainly include It is...
Embodiment 1
[0042] Example 1: Effects of different tissues on hemostatic materials
[0043] 1) Use pig skin, pig liver, pig kidney, rabbit skin and fish skin respectively for decellularization treatment and prepare hemostatic materials.
[0044] 2) After decellularization treatment, clean it, freeze-dry it and pulverize it to obtain tissue powder;
[0045]3) Add tissue powder to the solution, add pepsin for digestion for 8-36 hours (fish skin 8 hours, rabbit skin 12 hours, pig liver and kidney 12 hours, pig skin 24 hours); after digestion, adjust the pH to 7.2-7.4;
[0046] 4) Then add phosphate buffered saline PBS in the digestion solution, so that the concentration of phosphate buffered saline in the digestion solution is 0.001 M, and the treatment time is 15min;
[0047] 5) After PBS treatment, washing, drying, molding, and preparing a hemostatic sponge material.
[0048] Appearance photos of hemostatic materials such as figure 1 shown. Tissues from different sources can be process...
Embodiment 2
[0049] Example 2: Effects of different decellularization methods on hemostatic materials
[0050] The pig skin was decellularized by stirring method, chemical method (same as in Example 1) and biological enzyme method, and then a sheet-shaped hemostatic material was prepared according to the same method (same as in Example 1). A rat liver hemorrhage model was prepared, the rat liver was exposed, a hole punch was punched into a round hole with a diameter of 0.8 cm and a depth of 3 mm, and hemostatic materials were put on to observe the hemostatic effect.
[0051] The result is as figure 2 As shown (in the figure, A is the liver hemorrhage model, the left picture is the control group, the blood coagulates without adding material, the right picture is the hemostatic material used for liver hemostasis; the left picture of B is the liver bleeding time of the control group and the hemostatic material group Determination, hemostatic material significantly shortened the bleeding tim...
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