Exopolysaccharide sewage treatment flocculant and preparation method thereof
An exopolysaccharide and sewage treatment technology, which is used in water/sewage treatment, water treatment parameter control, water/sludge/sewage treatment, etc. , cell carcinogenesis and other problems, to achieve the effects of good biodegradation performance, rich flocculation forms, and easy preparation
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Embodiment 1
[0022] 1. Microbial exopolysaccharide preparation
[0023] (1) Fermentation reaction medium:
[0024] Sucrose 65g / L; potassium dihydrogen phosphate 5g / L; manganese sulfate 0.03g / L; magnesium sulfate 0.8g / L; ferric nitrate 0.05g / L; calcium chloride 0.3g / L; yeast powder 10g / L; peptone 18g / L; distilled water.
[0025] (2) Preparation of exopolysaccharide by fermentation
[0026] The culture medium was prepared according to the formula ratio of the fermentation reaction medium, and then sterilized at 120°C for 15 minutes to obtain the fermentation reaction medium. Bacillus licheniformis was inoculated into the fermentation reaction medium with an inoculum size of 5%, at 33 ℃, 160rpm shaking culture for 5 days, centrifugation to obtain the fermentation supernatant; dialyze the fermentation supernatant, and then centrifuge to obtain the dialyzed supernatant; filter the dialyzed supernatant, vacuum freeze-dry to obtain the cell exopolysaccharides.
[0027] 2. Preparation of silk...
Embodiment 2
[0042] 1. Microbial exopolysaccharide preparation
[0043] (1) Fermentation reaction medium:
[0044]Sucrose 60g / L; Potassium dihydrogen phosphate 3g / L; Manganese sulfate 0.01g / L; Magnesium sulfate 0.5g / L; Ferric nitrate 0.07g / L; Calcium chloride 0.5g / L; Yeast powder 10g / L; Peptone 18g / L; distilled water.
[0045] (2) Preparation of exopolysaccharide by fermentation
[0046] Prepare the culture medium according to the formula ratio of the fermentation reaction medium, and then carry out sterilizing treatment at 120° C. for 15 minutes to obtain the fermentation reaction medium. Bacillus licheniformis is inoculated into the fermentation reaction medium with an inoculum size of 5%, at 32 ℃, 200rpm shaking culture for 5 days, centrifugation to obtain the fermentation supernatant; dialyze the fermentation supernatant, and then centrifuge to obtain the dialyzed supernatant; filter the dialyzed supernatant, vacuum freeze-dry to obtain the cell exopolysaccharides.
[0047] 2. Pre...
Embodiment 3
[0061] 1. Microbial exopolysaccharide preparation
[0062] (1) Fermentation reaction medium:
[0063] Sucrose 70g / L; Potassium dihydrogen phosphate 6g / L; Manganese sulfate 0.05g / L; Magnesium sulfate 1.0g / L; Ferric nitrate 0.03g / L; Calcium chloride 0.2g / L; Yeast powder 13g / L; Peptone 15g / L; distilled water.
[0064] (2) Preparation of exopolysaccharide by fermentation
[0065] The culture medium was prepared according to the formula ratio of the fermentation reaction medium, and then sterilized at 120°C for 15 minutes to obtain the fermentation reaction medium. Bacillus licheniformis was inoculated into the fermentation reaction medium with an inoculum size of 5%, at 35 ℃, 160rpm shaking culture for 4 days, centrifugation to obtain the fermentation supernatant; dialyze the fermentation supernatant, and then centrifuge to obtain the dialyzed supernatant; filter the dialyzed supernatant, vacuum freeze-dry to obtain the cell exopolysaccharides.
[0066] 2. Preparation of silk...
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