A marine self-flocculating bacterium and its development-driven salt-adapted nitrogen assimilation microbiome and its construction method and application

A construction method and nitrogen assimilation technology, applied in the field of microbial and environmental governance and restoration, can solve problems such as unclear metabolic characteristics, long sludge domestication time, complex regulation methods, etc., and achieve high removal efficiency, good organic matter and phosphorus removal capacity , the effect of a wide range of application prospects

Active Publication Date: 2022-08-02
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the inventors found that the sludge used in this method has various microbial communities, unclear metabolic characteristics, complex control methods, long sludge domestication time, and slow system startup.

Method used

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  • A marine self-flocculating bacterium and its development-driven salt-adapted nitrogen assimilation microbiome and its construction method and application
  • A marine self-flocculating bacterium and its development-driven salt-adapted nitrogen assimilation microbiome and its construction method and application
  • A marine self-flocculating bacterium and its development-driven salt-adapted nitrogen assimilation microbiome and its construction method and application

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preparation example Construction

[0076] d) preparation of organic fertilizers;

[0077] e) improvement of soil fertility;

[0078] f) Bioremediation of saline-alkali land;

[0079] g) Governance of water eutrophication;

[0080] h) Water pollution remediation;

[0081] i) Greenhouse gas reduction and carbon neutrality;

[0082] Wherein, in the b), nitrogen is metabolized based on assimilation;

[0083] In described c), the waste water includes high-salt sewage, marine aquaculture waste water, industrial salt-containing waste water, and seawater toilet flushing waste water;

[0084] In the above g), the water body includes fresh water and sea water, preferably sea water.

[0085] In yet another specific embodiment of the present invention, there is provided a method for the integration and resource transformation of nutrients in a high-salt environment, the method comprising: applying the above-mentioned Psychrobacteraquimaris A4N01, microorganism Inoculants and / or salt-adapted nitrogen assimilation micr...

Embodiment 1

[0089] Screening and culturing method of Psychrobacter aquimaris A4N01, the steps are as follows:

[0090] The marine sediments taken from the Yellow Sea (122°48'E, 35°59'N) were inoculated into seawater LB medium, and cultured in a shaker at 25°C and 200r / min for 24h. The supernatant was streaked on a seawater LB solid plate.

[0091] The single colonies formed on the plates were respectively inoculated into seawater LB medium, and shaken for 24 hours in a shaker at 25°C and 200 r / min, and the microorganisms with flocculation ability were observed and screened.

[0092] The bacterial strain with flocculation ability obtained by the above screening is inoculated in the ammonia nitrogen medium, investigate its ammonia nitrogen and total nitrogen removal ability, and screen the bacterial strain with high ammonia nitrogen removal efficiency, no nitrite and nitrate production, and no loss of total nitrogen in the system, that is, It is the Psychrobacter aquimaris A4N01 of the pre...

Embodiment 2

[0095]The determination methods of nitrogen metabolism physicochemical properties of Psychrobacter aquimaris A4N01 strain are as follows

[0096] (1) Psychrobacter aquimaris A4N01 was inoculated into seawater LB medium and cultured in a shaker at 25°C and 200 r / min for 24 hours to obtain an activated bacterial solution. ① Take the prepared Psychrobacter aquimaris A4N01 activated bacterial solution and centrifuge it at 4000rpm for 10min; ②After removing the supernatant, use sterilized saline (0.85%NaCl) to resuspend and wash the bacteria; Centrifuge at 4000rpm for 10min. ③ After resuspending the bacterial cells with sterilized saline, inoculate them in the medium containing ammonia nitrogen, nitrous nitrogen and urea as nitrogen sources according to 10% of the inoculum, and take samples according to time points to determine the effect of the strain on various nitrogen sources. utilization and transformation.

[0097] The ammonia nitrogen, nitrous nitrogen and urea medium in th...

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Abstract

The invention provides a marine self-flocculating bacteria and a salt-adapted nitrogen assimilation microbiome, a construction method and application thereof, which belong to the technical field of microorganism and environmental treatment and restoration. In the present invention, a seawater psychrophilic bacillus (Psychrobacter aquimaris) A4N01 is screened from marine sediments, which can assimilate ammonia nitrogen to synthesize organic nitrogen, and simultaneously transform organic matter and phosphorus, and the salt-adapted nitrogen assimilation microbial group developed by it has good nutrition Substance conversion removal ability and sedimentation performance, wide tolerance to salinity, can be used in high-salt sewage treatment, saline-alkali soil fertilization and other directions, to achieve the recovery of nitrogen, phosphorus and other nutrients in high-salt environment, simple operation, low price, Environmentally friendly, so it has good practical application value.

Description

technical field [0001] The invention belongs to the technical field of microorganisms and environmental treatment and restoration, and in particular relates to a marine self-flocculating bacteria and a salt-adapted nitrogen assimilation microbiome driven by its development, a construction method and application. Background technique [0002] The information disclosed in this Background section is only for enhancement of understanding of the general background of the invention and should not necessarily be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art. [0003] The transformation of nutrients in a high-salt environment is easily inhibited by salinity, and there are problems such as difficult treatment of high-salt sewage, difficult transformation of substances in saline-alkali land, and easy loss of nutrients. However, since high-salt sewage contains a large amount of nitr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/36C02F3/34C12R1/01
CPCC12N1/20C12N1/36C02F3/34C12N1/205C02F2101/16C02F2103/005C02F2103/08C02F3/1263C12R2001/01
Inventor 周维芝张梦汝韩飞刘喆
Owner SHANDONG UNIV
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