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Method for producing agilawood by utilizing plant cell culture fermentation and application of agilawood in cosmetics

A plant cell and agarwood technology, applied in microorganism-based methods, cosmetics, cosmetic preparations, etc., can solve the problems of long plant growth cycle, shortage of raw material resources, and large environmental impact, and achieve fast growth speed, high-efficiency raw material preparation, The effect of less post-treatment pollution

Active Publication Date: 2021-09-24
APPLE FLAVOR & FRAGRANCE GRP
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] The purpose of the present invention is to develop a new method of producing agarwood using plant cell culture technology and apply it to cosmetics, so as to solve the problems of long growth cycle of traditional plants, shortage of raw material resources, and great influence from the environment, and provide A new idea for the application of agarwood in cosmetics

Method used

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  • Method for producing agilawood by utilizing plant cell culture fermentation and application of agilawood in cosmetics
  • Method for producing agilawood by utilizing plant cell culture fermentation and application of agilawood in cosmetics
  • Method for producing agilawood by utilizing plant cell culture fermentation and application of agilawood in cosmetics

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Embodiment 1

[0046] A kind of method utilizing plant cell suspension culture technique to produce agarwood, its steps are as follows:

[0047] (1) Preparation of Ayurveda alba callus induction medium

[0048] The induction medium consists of B5 basal salt medium, sucrose 20-30g / L, agar 6-8g / L, inositol 80-120mg / L, 6-benzylaminopurine (6-BA) 2-3.5mg / L, α - Naphthaleneacetic acid (NAA) 0.2-0.6mg / L, indole-3-acetic acid (IAA) 0.4-1.2mg / L, choline 1-1.8g / L and water to make induction medium;

[0049] (2) Preparation method for inducing callus of A. japonica

[0050] Take the newly germinated stems of A. japonica plants, rinse the young stems of A. japonica with clean water for about 2 hours, and then cut them into about 1cm with sterilized scissors in a sterile ultra-clean bench. Use 70% Soak in ethanol for 30s, then soak in 4% sodium hypochlorite solution for 15-20min, then connect to tissue culture flasks containing hormones (6-benzylaminopurine (6-BA) and α-naphthaleneacetic acid (NAA)) f...

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Abstract

The invention relates to the technical field of plant cell culture, and discloses a method for producing agilawood by utilizing plant cell culture fermentation and application of the agilawood in cosmetics. The method comprises the following steps of: preparation of a culture medium, induction culture of aquilaria sinensis embryonic callus, fermentation culture, extraction and identification of a product, and preparation of agilawood balm. According to the method, the plant cell culture technology is adopted, induction preparation of suspension culture embryonic callus is realized, aquilaria sinensis callus cells can be provided for large-scale culture, the agilawood is further prepared by utilizing a solid fermentation method, the operation is simple, the raw material preparation is efficient, the post-treatment pollution is less, and the method is close to the agilawood generating process of aquilaria sinensis in a natural environment. The agilawood extract provided by the invention has excellent effects of relieving emotion and improving sleep, and can be used as a compound cosmetic and / or a skin care product additive.

Description

technical field [0001] The invention relates to the technical field of plant cell culture, in particular to a method for producing agarwood by fermenting the embryogenic callus of A. japonica through plant cell culture technology and its application in cosmetics. Background technique [0002] Agarwood (gaharu, agarwood, eaglewood) is a traditional precious spice and medicinal material in China, Japan, India, Southeast Asia, and the Middle East. It is mainly derived from the Aquilaria plant of the family Thymelaeaceae. Currently, there are 19 species recognized as producing aroma. . At this stage, all Agarwood plants are included in Appendix II of the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES). In 2020, Agarwood is also listed by Hainan Province as the "fourth tree" after betel nut, coconut and rubber. "Focus on the development. [0003] Aquilariasinensis (Lour.) Gilg., also known as Aquilaria sinensis, is the unique and only tre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61Q19/00A61P25/20A61P25/00A61P25/22C12P1/02C12N1/14C12R1/77
CPCA61K8/9789A61Q19/00A61P25/20A61P25/00A61P25/22C12P1/02C12N1/14A61K2800/85
Inventor 徐菱艳卓之阳黄健黄彦宾孟宪乐王玥
Owner APPLE FLAVOR & FRAGRANCE GRP
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