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Enzyme and microecological preparation composite agent and preparation method thereof

A micro-ecological preparation and enzyme mixture technology, applied in the field of feed additives, can solve the problems of unstable gastric acid and bile salts, intolerance to antibiotics, and high water content, and achieve the improvement of intestinal flora, comprehensive efficacy and long shelf life. Effect

Pending Publication Date: 2021-09-28
SHANXI AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are also certain problems in probiotics, such as low viable bacteria content, high water content, intolerance to antibiotics, instability to gastric acid and bile salts, etc., which affect its application effect on livestock and poultry, and limit probiotics Applications
[0007] Therefore, so far, stable and efficient compound formulations composed of suitable enzymes and probiotics have yet to be developed.

Method used

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  • Enzyme and microecological preparation composite agent and preparation method thereof
  • Enzyme and microecological preparation composite agent and preparation method thereof
  • Enzyme and microecological preparation composite agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1 Preparation of enzyme and probiotics compound composition.

[0046] Inoculate Bacillus subtilis into 100mL LB liquid medium, and cultivate until the concentration of the bacterial solution reaches 10 7 CFU / mL, inoculate Saccharomyces cerevisiae into 100mL PDB medium, cultivate until the concentration of bacteria liquid reaches 10 7 CFU / mL, inoculate Lactobacillus acidophilus into 100mL MRS liquid medium, and cultivate until the concentration of bacteria reaches 10 7 CFU / mL.

[0047] Take 5mL of the cultured Bacillus subtilis bacteria liquid and add it to the fermentation medium, add 0.1g of neutral protease and 0.3g of cellulase, and place it in a shaker with a rotation speed of 200rpm and a temperature of 36°C under anaerobic conditions. Cultivate for 14 hours to make a mixture of Bacillus subtilis; take 5mL of cultured Saccharomyces cerevisiae and add it to the fermentation medium, and add 0.2g of neutral protease and 0.3g of cellulase. Cultivate in a ...

Embodiment 2

[0050] Example 2 The preparation of the composite composition of enzyme and probiotics.

[0051] Inoculate Bacillus subtilis into 100mL LB liquid medium, and cultivate until the concentration of the bacterial solution reaches 10 7 CFU / mL, inoculate Saccharomyces cerevisiae into 100mL PDB medium, cultivate until the concentration of bacteria liquid reaches 10 7 CFU / mL, inoculate Lactobacillus acidophilus into 100mL MRS liquid medium, and cultivate until the concentration of bacteria reaches 10 7 CFU / mL.

[0052] Take 5mL of the cultured Bacillus subtilis bacteria liquid and add it to the fermentation medium, add 0.1g of neutral protease and 0.3g of cellulase, and place it in a shaker with a rotation speed of 200rpm and a temperature of 36°C under anaerobic conditions. Cultivate for 14 hours to make a mixture of Bacillus subtilis; take 5mL of cultured Saccharomyces cerevisiae and add it to the fermentation medium, and add 0.2g of neutral protease and 0.3g of cellulase. Cultiv...

Embodiment 3

[0055] Example 3 The preparation of the composite composition of enzyme and probiotics.

[0056] Inoculate Bacillus subtilis into 100mL LB liquid medium, and cultivate until the concentration of the bacterial solution reaches 10 7 CFU / mL, inoculate Saccharomyces cerevisiae into 100mL PDB medium, cultivate until the concentration of bacteria liquid reaches 10 7 CFU / mL, inoculate Lactobacillus acidophilus into 100mL MRS liquid medium, and cultivate until the concentration of bacteria reaches 10 7 CFU / mL.

[0057] Take 5mL of the cultured Bacillus subtilis bacteria liquid and add it to the fermentation medium, add 0.1g of neutral protease and 0.3g of cellulase, and place it in a shaker with a rotation speed of 200rpm and a temperature of 36°C under anaerobic conditions. Cultivate for 14 hours to make a mixture of Bacillus subtilis; take 5mL of cultured Saccharomyces cerevisiae and add it to the fermentation medium, and add 0.2g of neutral protease and 0.3g of cellulase. Cultiv...

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Abstract

The invention discloses an enzyme and microecological preparation composite agent and a preparation method thereof. The enzyme and microecological preparation composite agent is prepared by mixing a bacillus subtilis mixed solution, a saccharomyces cerevisiae mixed solution and a lactobacillus acidophilus mixed solution to obtain a bacterium and enzyme mixed solution, and then mixing the bacterium and enzyme mixed solution with a protective agent. The obtained composite agent can regulate the ecological balance of animal intestinal tracts, prevent diseases, promote digestion and improve the utilization rate of feed nutrient substances, and can reach a to-be-released position on the premise of completely protecting each component in the agent from being influenced by the outside or an in-vivo environment.

Description

technical field [0001] The invention relates to the field of feed additives, in particular to a composite composition of probiotics and enzymes for improving the utilization rate of feed nutrients and a preparation method. Background technique [0002] Probiotics are beneficial additives that can be added to animal feed. Targeted probiotics can be selected for different animals or their states to degrade substances that are difficult or indigestible for animals and improve the utilization of feed nutrients. [0003] With the rapid development of animal husbandry, the types and usage of antibiotics in animal feed are increasing, and antibiotics have even become an essential additive to improve animal growth. However, with the abuse and irrational use of antibiotics, more and more drug-resistant bacteria have emerged, which greatly reduces the efficacy of commonly used animal drugs, or even has no effect. The drug resistance of pathogenic bacteria, drug toxicity, and drug res...

Claims

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Application Information

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IPC IPC(8): A23K10/18A23K20/189A23K50/30A23K50/75C12N1/20C12N1/16C12N9/50C12N9/42C12R1/125C12R1/865C12R1/23
CPCA23K10/18A23K20/189A23K50/30A23K50/75C12N1/20C12N1/16C12N9/50C12N9/2437A23V2400/113
Inventor 任玉红裴婷王海东耿建军程志学于秀菊
Owner SHANXI AGRI UNIV
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