Method for improving soil through combined action of mixed fungicide and chemical fertilizer
A technology of mixed bacterial agents and combined effects, which is applied in chemical instruments and methods, organic fertilizers, fertilizer mixtures, etc., can solve the problems of occupying the living space of beneficial microorganisms, crop susceptibility to disease, and crop growth restrictions, etc., to improve the utilization rate of chemical fertilizers , Improve soil air permeability and reduce soil pollution
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[0034] Wherein, the preparation method of described bacillus subtilis and bacillus amyloliquefaciens inoculum comprises the following steps:
[0035] (1) Strain activation: Inoculate the low-temperature-preserved Bacillus subtilis and Bacillus amyloliquefaciens strains on the plate medium respectively, and culture them at 28-39°C for 24-26 hours; then pick a single colony and inoculate them on the slant medium and cultured at 30-39°C for 24-26 hours, then washed the surface of the medium with sterile water, and the eluate was used as the inoculum;
[0036] (2) Preparation of seed solution: add the inoculation solution prepared in step (1) into the liquid medium according to the volume percentage of 1-10%, shake and cultivate at 26-36° C. for 12-18 hours to obtain the seed solution;
[0037](3) Fermentation culture: the seed solution obtained in step (2) is inserted into the corn flour bean cake powder medium according to the volume percentage of 3-10%, and the temperature is 2...
Embodiment 1
[0039] A method for improving soil through the joint action of mixed bacterial agents and chemical fertilizers, comprising the steps of:
[0040] Step 1: Put the corn stalks into the pulverizer for crushing, crush the corn stalks to a length of less than 5 cm, then thermally crack the corn stalks at 500°C under anaerobic conditions, and continue the thermal cracking for 3 hours , prepared into biochar after natural cooling;
[0041] Step 2: Apply the mixed bacterial agent to the soil at a rate of 200 kg / mu evenly 30 days before the farmland is cultivated, and fully mix the soil in the plow layer with the mixed bacterial agent. The mixed bacterial agent is composed of the following components: 1500 The biochar of weight part and the composite microbial bacterial agent of 10 weight parts, described composite microbial bacterial agent is made up of Bacillus subtilis, Bacillus licheniformis, Bacillus lateral sporosum, Bacillus megaterium, Bacillus amyloliquefaciens, Trichoderma ha...
Embodiment 2
[0044] A method for improving soil through the joint action of mixed bacterial agents and chemical fertilizers, comprising the steps of:
[0045] Step 1: Put the corn stalks into the pulverizer for crushing, crush the corn stalks to a length of less than 5 cm, then thermally crack the corn stalks at 500°C under anaerobic conditions, and continue the thermal cracking for 3 hours , prepared into biochar after natural cooling;
[0046] Step 2: Apply the mixed bacterial agent to the soil at a rate of 200 kg / mu evenly 30 days before the farmland is cultivated, and fully mix the soil in the plow layer with the mixed bacterial agent. The mixed bacterial agent is composed of the following components: 1500 The biochar of weight part and the composite microbial bacterial agent of 10 weight parts, described composite microbial bacterial agent is made up of Bacillus subtilis, Bacillus licheniformis, Bacillus lateral sporosum, Bacillus megaterium, Bacillus amyloliquefaciens, Trichoderma ha...
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