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An amino acid and sequence technology, applied in the field of peptides, can solve the problem of not directly promoting wound regeneration
Active Publication Date: 2022-08-09
WEST CHINA HOSPITAL SICHUAN UNIV
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Problems solved by technology
[0003] The treatment of chronic wounds is mainly for wound management, but does not directly promote wound regeneration
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Embodiment 1
[0053] Example 1 Preparation of KGH hydrogel of the present invention
[0054] According to the treatment requirements of the present invention, according to the preliminary experimental results (therapeutic effect), experimental animals (C57 mice) and application sites (mice skin wounds), the treatment system is determined as follows:
[0055]
[0056] 1) Self-assembled short peptide KGH (sequence: Ac-KLDLKLDLKLDLGGH-CONH 2 , as shown in SEQ ID No. 1) synthesized and purified by Shanghai Botai Biotechnology, the dry powder was dissolved in sterile water, prepared into a 10 mg / mL stock solution, and 30 μL was added to the skin wound;
[0057] 2) Slowly add 10 μL of normal saline to the wound surface to form a hydrogel.
Embodiment 2
[0058] Example 2 Distribution experiment of KGH hydrogel of the present invention in skin wound
[0059] 1) Experimental materials
[0060] Self-assembled short peptide KGH (sequence: Ac-KLDLKLDLKLDLGGH-CONH 2 )
[0094] Animal grouping: DM(+NS), DM+KGH two groups
[0095] Mice were fasted for 12 hours before STZ injection and had free access to water, and each mouse was injected with 150 mg / kg of STZ intraperitoneally;
[0096] 3d after injection, the random...
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Abstract
The invention relates to the field of pharmaceutical preparations, in particular to a polypeptide hydrogel for promoting skin repair and a preparation method and application thereof. The present invention provides a self-assembled peptide, and also provides a polypeptide hydrogel formed from an aqueous solution of the self-assembled peptide. The present invention also provides a method for preparing the aforementioned polypeptide hydrogel. The present invention also provides the use of the aforementioned polypeptide hydrogel in the treatment of skin damage in diabetic mice. Among them, the polypeptide hydrogel can promote the healing of damaged skin of diabetic mice, the formation of skin appendages, increase the positive rate of PCNA cells, and promote the accumulation of CD31-positive blood vessels and collagen. The present invention also provides the use of the aforementioned polypeptide hydrogel for 3D cell culture in vitro; wherein, the human keratinocyte line HaCaT cells grow spherically in the polypeptide hydrogel, and the expression levels of PDGF and VEGF in the cells are relatively two-dimensional. Cultured cells are high.
Description
technical field [0001] The present invention relates to the field of pharmaceutical preparations, in particular to a polypeptide. Background technique [0002] A chronic wound refers to the failure of the damaged skin and surrounding soft tissue to heal for more than 3 months. It is caused by persistent and aggravated inflammatory processes due to the specificity of the wound, such as bedsores / pressure ulcers and poor healing of burns / scalds; or as a complication of diseases such as diabetes, vascular insufficiency and immunodeficiency, such as diabetic foot and venous ulcers. Due to the lack of effective treatments, chronic trauma has high morbidity and mortality, and creates a huge social / medical burden as well as a physical / economic burden for the patient. The normal wound healing process is divided into hemostatic, inflammatory, proliferative and remodeling phases, however in chronic wounds these healing processes are hindered and ultimately lead to poor skin barrier, ...
Claims
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Application Information
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