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Body fluid detection method and system based on SERS

A detection method and body fluid technology, applied in the field of SERS-based body fluid detection method and system, can solve the problem of unsuitability for physical examination and continuous tracking of disease, lack of repeatability, stability and accuracy, and unsuitability for high-frequency general survey of large-scale population and other issues to achieve the effect of saving data processing time, improving accuracy and reliability, and overcoming instability and non-repeatability

Active Publication Date: 2021-10-19
TSINGHUA UNIV
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Problems solved by technology

However, this method can only test a single substance, which is expensive, and there are still many unknown disease-specific substances, which cannot be used for screening of various diseases, and is not suitable for general physical examination and continuous follow-up of the disease
In terms of analysis, since the substrate is not completely consistent and uniform, the surface enhancement factors at different positions are not the same, so the change of substance content cannot be judged by simply comparing the change of peak intensity. In addition, there is also the influence of fluorescence in body fluid samples
[0007] In summary, the current surface-enhanced Raman liquid biopsy method is lacking in repeatability, stability and accuracy, and the cost is high, so it is not suitable for high-frequency census of large-scale population

Method used

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  • Body fluid detection method and system based on SERS
  • Body fluid detection method and system based on SERS
  • Body fluid detection method and system based on SERS

Examples

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Embodiment 1

[0064] A method for detecting body fluid based on SERS, comprising the steps of:

[0065] S1. Drop the body fluid sample to be tested on the surface enhancement substrate, and specify the body fluid sample dropping area and measurement area;

[0066] The main steps include:

[0067] S11, the surface-enhanced Raman (SERS) substrate was prepared by the electrochemical redox roughening method, and the rough substrate was cleaned and dried by ultrasonic vibration, and divided into regions and grids; specifically, the SERS substrate materials include but are not limited to gold, silver, Materials such as copper or graphene, substrates with surface-enhanced Raman scattering effects include but are not limited to rough silver substrates, rough gold substrates, or other achievable surface-enhanced Raman scattering substrates and nanoparticles. The embodiment uses metallic silver as the SERS substrate representative of the material.

[0068] In this embodiment, if necessary, specific...

Embodiment 2

[0107] A body fluid detection system based on SERS, comprising:

[0108] Baseline processing module: The baseline processing module is used to eliminate the influence of fluorescence and flatten the baseline. The processing methods include but are not limited to dual wavelengths to eliminate the influence of fluorescence, piecewise linear interpolation, polynomial fitting, etc.;

[0109] Specifically, the embodiment uses piecewise linear interpolation as the representative of the baseline processing method, divides the abscissa of the Raman spectrum, that is, the Raman frequency shift, into multiple intervals, and finds a minimum point within a certain wavenumber range before and after each interval , use these two points as the reference to do linear interpolation in the interval, and get the baseline spectral line by fitting. The baseline data can be subtracted from the original spectral data to achieve a flattened baseline. In actual data analysis and processing, you can als...

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Abstract

The invention provides a body fluid detection method and system based on SERS, the detection method comprises a laser correction large light spot integral measurement mode and a rapid spot scanning measurement mode, and the laser correction large light spot integral measurement mode can rapidly obtain stable and repeatable Raman spectrum results; and a fast spot scan pattern can capture low concentrations of disease-related markers in body fluids. Based on the above two measurement modes, the method can realize accurate detection of overall components and differential components of the body fluid. The system provided by the invention comprises a baseline processing method, a spectrum standardization method, an automatic peak searching method and an imaging method, and realizes rapid, objective and large-batch processing of spectrum data and automatic acquisition of difference characteristic peaks. Rapid, stable, high-throughput and low-cost surface enhanced Raman body fluid detection is effectively realized, rapid disease pre-diagnosis can be carried out to replace a traditional body fluid detection method by detecting and analyzing Raman spectrum characteristic information of human body fluid, and high-frequency general survey of large-area crowds is realized.

Description

technical field [0001] The invention relates to the technical field of Raman measurement, in particular to a SERS-based body fluid detection method and system. Background technique [0002] In recent years, the morbidity and mortality of cancer in my country have continued to rise, and it is gradually becoming younger, which seriously threatens the life and health of our people. Early detection, early treatment, and early diagnosis are the key to cancer treatment, which can significantly improve the cure rate of cancer, and the five-year survival rate can reach 90%, while the five-year survival rate of advanced cancer is only 10-20%. [0003] Liquid biopsy is an important method for early cancer screening. Liquid biopsy is mainly for the detection of human body fluids. Human body fluids include serum, plasma, urine, saliva, lymph, feces extracts, etc., which contain a large number of substances related to diseases. When cancer occurs, the composition and concentration of ...

Claims

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Application Information

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IPC IPC(8): G01N21/65C25F3/02
CPCG01N21/658C25F3/02
Inventor 张兴张晓宇樊傲然
Owner TSINGHUA UNIV
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