Novel photoelectric biosensing system for detecting 17 beta-estradiol
A bio-sensing and estradiol technology, applied in measurement devices, material electrochemical variables, material analysis by electromagnetic means, etc., can solve problems such as E2 emission, and achieve low cost of consumables, easy control of reaction conditions, and stability. Good results
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[0016] (1) Electrochemical deposition of FeOOH layer: The three electrodes of FTO, Pt and Ag / AgCl were immersed in 0.1 MFeSO4 7H2O electrolyte as a group to form an electrochemical deposition device. Under the condition of no simulated light, the electrochemical deposition potential and The time is controlled at 1.2 V vs Ag / AgCl and 10-20 min, respectively;
[0017] (2) Preparation of FTO / FeOOH / CoTiO3: Mix 0.01 mol Co(CH3COO)2 4H2O and 0.01 mol tetrabutyl titanate with 60 mL ethylene glycol, stir for 24 h, and centrifuge the uniformly stirred CoTiO3 precursor solution Three times, washed with ethanol, dried in vacuum at 80 °C for 4 h, finally, the powder was heat-treated in air for 2 h at 600 °C, 20 μL of CoTiO3 solution (8 mg / mL) was modified on the electrode and Dry to form FTO / FeOOH / CoTiO3 electrode;
[0018] (3) Preparation of biosensing electrodes: Then modify the electrodes with 6 μL of 0.5% chitosan solution containing 1% acetic acid, semi-dry in the air, and then rins...
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