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Radix pseudostellariae polysaccharide and preparation method thereof

A technology of Radix Pseudostellariae and polysaccharides, which can be used in medical formulas, medical preparations containing active ingredients, plant raw materials, etc. It can solve problems such as complex ingredients, unknown content and purity of active ingredients, negative impact on product flavor, and taste, and achieve restoration. Effects of histopathological damage, ease of expression level, and high purity

Inactive Publication Date: 2021-10-22
ANHUI WANNAN TOBACCO LEAF +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are certain disadvantages in these preparation methods: on the one hand, the extract of Pseudostellaria Pseudostellariae obtained by traditional methods such as boiling and soaking still has a prominent earthy smell and traditional Chinese medicine smell, which has a negative effect on the flavor and taste of the product. It is not easy to be accepted by consumers; on the other hand, the content and purity of active ingredients are unknown, and the ingredients are complex, which is not conducive to product quality control

Method used

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  • Radix pseudostellariae polysaccharide and preparation method thereof
  • Radix pseudostellariae polysaccharide and preparation method thereof
  • Radix pseudostellariae polysaccharide and preparation method thereof

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preparation example Construction

[0042] The present invention provides a kind of preparation method of heterophylla polysaccharide, comprising the following steps:

[0043] (1) drying and crushing Pseudostellariae Pseudostellariae powder to obtain Pseudostellariae powder;

[0044] (2) Carry out water extraction and concentration to the gained Radix Pseudostellariae powder successively, obtain concentrated solution;

[0045] (3) carrying out alcohol precipitation to the obtained concentrated solution, and obtaining the crude polysaccharide of Pseudostellaria Pseudostellariae after freeze-drying the obtained precipitate;

[0046] (4) performing purification, elution, dialysis, concentration and freeze-drying sequentially on the obtained Pseudostellaria Pseudostellaria polysaccharides to obtain Pseudostellaria polysaccharides.

[0047] In the present invention, the particle size of the Pseudostellariae powder in the step (1) is preferably 40-60 mesh, more preferably 50 mesh.

[0048] In the present invention, ...

Embodiment 1

[0060] 1) Dry the Radix Pseudostellariae, crush it with a high-speed crusher, and pass through a 40-mesh sieve to obtain Radix Pseudostellaria powder;

[0061] 2) Add 8 times of distilled water to Pseudostellariae powder, extract at 80°C for 100 minutes, centrifuge, extract twice, combine the filtrate, concentrate to 1 / 11 of the original volume, and then use ethanol with a volume fraction of 75%, ethanol at 3°C Settled for 12 hours, centrifuged to obtain the precipitate, and freeze-dried at -40°C for 48 hours to obtain the crude polysaccharide of Radix Pseudostellariae;

[0062] 3) Use DEAE Cellulose-52 ion exchange column to purify the polysaccharide. The purification condition is: Tris-HCl buffer solution with pH 7.5 is used to load the sample. 0, 0.1, 0.2, 0.3mol / L NaCl salt solution is eluted in equal volumes, the flow rate is 1.5mL / min, the first single elution peak is collected, 450Da dialysis bag is dialyzed to remove salt, and concentrated to 1 / 11 of the original volum...

Embodiment 2

[0064] 1) Dry the Radix Pseudostellariae, crush it with a high-speed crusher, and pass through a 50-mesh sieve to obtain Radix Pseudostellaria powder;

[0065] 2) Add 9 times of distilled water to the Pseudostellariae powder, extract at 100°C for 120 minutes, centrifuge, extract 3 times, combine the filtrate, concentrate to 1 / 10 of the original volume, and then use ethanol with a volume fraction of 80%, ethanol at 4°C Settled for 14 hours, centrifuged to obtain the precipitate, and freeze-dried at -30°C for 60 hours to obtain the crude polysaccharide of Radix Pseudostellariae;

[0066] 3) Use DEAE Cellulose-52 ion exchange column to purify the polysaccharide. The purification conditions are: Tris-HCl buffer solution with pH 7.6 is used to load the sample, and the concentration of the sample is 1.5 mg / mL Pseudostellaria polysaccharide. The salt solution containing 0, 0.1, 0.2, 0.3mol / L NaCl is eluted in equal volumes, the flow rate is 2mL / min, the first single elution peak is c...

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Abstract

The invention belongs to the technical field of polysaccharide preparation, and provides radix pseudostellariae polysaccharide and a preparation method thereof. The method comprises the steps of drying and crushing, water extraction, concentration, alcohol precipitation, freeze drying, purification, elution, dialysis and the like. The method can effectively cover the inherent earthy smell, traditional Chinese medicine smell and other bad flavors of the radix pseudostellariae, and the purity is high. The research verifies that the radix pseudostellariae polysaccharide prepared by the invention can also regulate the body weight growth of the mouse, repair tissue pathological injury and relieve the inflammation expression level of the body, and comprehensively shows that the radix pseudostellariae polysaccharide has the capability of regulating the in-vivo immunity of the mouse.

Description

technical field [0001] The invention relates to the technical field of polysaccharide preparation, in particular to a heterophylla polysaccharide and a preparation method thereof. Background technique [0002] Pseudostellaria ginseng, also known as "baby ginseng", has the effects of nourishing qi, invigorating the spleen and moistening the lungs. It is often used clinically for loss of appetite, recovery after illness, cough and other discomforts. It is often used as a nourishing product. Compared with ginseng and American ginseng, Pseudostellariae heterophylla has mild medicinal properties and is more suitable for children to eat. Pseudostellaria polysaccharides are one of the main active substances in Pseudostellariae Radix. Studies have shown that Pseudostellaria polysaccharides have various health protection effects, such as regulating immunity, anti-oxidation, anti-fatigue, anti-tumor, and lowering blood sugar. [0003] At present, there are many methods for preparing ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61K36/36A61P3/10A61P35/00A61P37/02A61P39/00A61P39/06
CPCC08B37/0003A61K31/715A61K36/36A61P37/02A61P39/06A61P39/00A61P35/00A61P3/10
Inventor 徐海清李景明陈洋查道喜王学瑛徐刚朱启法李文保李世金秦祥林
Owner ANHUI WANNAN TOBACCO LEAF
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